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Sperm Preservation using Freeze-Drying Method

HAYATI Journal of Biosciences · Vol. 12 No. 1 (2009)

DOI
10.4308/hjb.12.1.41
ISSN
2086-4094, 1978-3019
Published
2009-11-30
Publisher
Bogor Agricultural University, Indonesia
Language
eng

Abstract

Since the discovery of cryopreservation method for bull semen, cryopreservation become an alternative method for maintaining gamet resources of certain animal which is threatened or near extinction. This technology was then applied to the preservation of embryo, oocyte, ovary and testis. The application of intracytoplasmic sperm injection (ICSI) for which sperm motility is unnecessary had supported the effort to create simplified method such as freeze-drying for sperm preservation. Due to the benefit of ICSI over the conventional in vitro fertilization (IVF) the spermatozoon could be mechanically driven to pass through the zona pellucida and entering the cytoplasm of oocytes prior to fertilization. The freeze-drying method is an alternative method in sperm preservation which ignored the motility of sperm. The sperm resulted from this technique is in drying state, therefore, it might be stored in room temperature or in refrigerator. Many reports have claimed that freeze-dried sperm which is not motile but has an intact DNA was able to fertilize oocytes, even produced offspring in mouse.

Collaboration network of the authors

40 authors · 122 co-authorship links · 1 across different organisations · showing the 40 most connected

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • Universitas Halu Oleo
  • IPB University
  • Hasanuddin University
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
La Ode NafiuUniversitas Halu Oleo5
Muhammad Amrullah PagalaUniversitas Halu Oleo4
Iman SupriatnaAffiliation unknown4
Rahim AkaUniversitas Halu Oleo3
Muhammad RizalUniversitas Halu Oleo3
HERDISAffiliation unknown3
Mokhamad FahrudinAffiliation unknown3
Berry JuliandiAffiliation unknown3
Achmad Selamet AkuUniversitas Halu Oleo3
ITA DJUWITAAffiliation unknown3
Luki AbdullahIPB University2
Asnath Maria FuahAffiliation unknown2
Sri RahayuHasanuddin University2
Rachmat SomanjayaAffiliation unknown2
Dian AgustinaAffiliation unknown2
Muh. RusdinUniversitas Halu Oleo2
Rusli BadaruddinUniversitas Halu Oleo2
Deki ZulkarnainUniversitas Halu Oleo2
Nur Santy AsminayaUniversitas Halu Oleo2
Andhika Yudha PrawiraAffiliation unknown2
Kelvin YapriantoAffiliation unknown2
Indra BachtiarAffiliation unknown2
Adi WinartoAffiliation unknown2
. YulnawatiAffiliation unknown2
Vista BudiariatiAffiliation unknown2
Dwi BudionoAffiliation unknown2
Ni Wayan Kurniani KarjaIPB University2
Harry MurtiAffiliation unknown2
Aryani Sismin SatyaningtijasAffiliation unknown2
Viny Aprilia KesumanengsihAffiliation unknown1
Nursanty AsminayaAffiliation unknown1
La Ode Abdul Karim IshakAffiliation unknown1
Meri AstutiAffiliation unknown1
Purnaning Dhian IsnaeniAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en">Sperm Preservation using Freeze-Drying Method</dc:title>
	<dc:creator xml:lang="en">SAILI, TAKDIR</dc:creator>
	<dc:creator xml:lang="en">PANGESTU, MULYOTO</dc:creator>
	<dc:creator xml:lang="en">SETIADI, MOHAMAD AGUS</dc:creator>
	<dc:creator xml:lang="en">AGUNGPRIYONO, SRIHADI</dc:creator>
	<dc:creator xml:lang="en">R. TOELIHERE, MOZES</dc:creator>
	<dc:creator xml:lang="en">BOEDIONO, ARIEF</dc:creator>
	<dc:description xml:lang="en">Since the discovery of cryopreservation method for bull semen, cryopreservation become an alternative method for maintaining gamet resources of certain animal which is threatened or near extinction. This technology was then applied to the preservation of embryo, oocyte, ovary and testis. The application of intracytoplasmic sperm injection (ICSI) for which sperm motility is unnecessary had supported the effort to create simplified method such as freeze-drying for sperm preservation. Due to the benefit of ICSI over the conventional in vitro fertilization (IVF) the spermatozoon could be mechanically driven to pass through the zona pellucida and entering the cytoplasm of oocytes prior to fertilization. The freeze-drying method is an alternative method in sperm preservation which ignored the motility of sperm. The sperm resulted from this technique is in drying state, therefore, it might be stored in room temperature or in refrigerator. Many reports have claimed that freeze-dried sperm which is not motile but has an intact DNA was able to fertilize oocytes, even produced offspring in mouse.</dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2009-11-30</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://journal.ipb.ac.id/hayati/article/view/170</dc:identifier>
	<dc:identifier>10.4308/hjb.12.1.41</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 12 No. 1 (2005): March 2005; 41</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://journal.ipb.ac.id/hayati/article/view/170/37</dc:relation>
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