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Direct Cloning of a Xylanase Gene from Pawan-Riau Hot Spring

HAYATI Journal of Biosciences · Vol. 14 No. 2 (2009)

DOI
10.4308/hjb.14.2.54
ISSN
2086-4094, 1978-3019
Published
2009-12-02
Publisher
Bogor Agricultural University, Indonesia
Language
eng

Abstract

A functional gene containing an Open Reading Frame (ORF) encoding a?-1, 4-endoxylanase glycosyl hydrolase family 11 was cloned directly using metagenomic PCR-cloning method from Pawan Hot Spring sample in Riau. The gene consisted of 642 nucleotides, encoded for 213 amino acids. The amino acid sequence analysis using BLAST showed that the gene has high homology (93%) with xylanase gene from Bacillus subtilis. The gene showed its function when it was subcloned into an expression vector and overexpressed in E. coli. The crude extract of the recombinant enzyme had activity for 170 U/ml at 50 oC. The result of this work showed that metagenomic approach was a powerful short cut method to obtain recombinant biocatalyst that was useful for industrial application. Key words:?-1, 4-endoxylanase, metagenomic DNA, Pawan-Riau hot-spring

Collaboration network of the authors

40 authors · 155 co-authorship links · showing the 40 most connected

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Co-authorOrganisationJoint articles
Maria UlfahAffiliation unknown6
Niknik NurhayatiAffiliation unknown4
Sri Rezeki WulandariAffiliation unknown4
Gabriela Christy SabbathiniAffiliation unknown3
Agustin Krisna WardaniAffiliation unknown2
Lina MulyawatiAffiliation unknown2
AbinawantoAffiliation unknown2
AMARILA MALIKAffiliation unknown2
Fairuz Andini FatiningtyasAffiliation unknown2
Riswanto NapitupuluAffiliation unknown2
Dadang SuhendarAffiliation unknown1
Anita Kusuma FinalissariAffiliation unknown1
Valerry Athalia PriyankaAffiliation unknown1
Khomaini HasanAffiliation unknown1
I Gede Eka Perdana PutraAffiliation unknown1
Mochamad NurcholisAffiliation unknown1
Muthia Rahmah DiniAffiliation unknown1
Kartika Sari CendanaAffiliation unknown1
Dini AchnafaniAffiliation unknown1
Sunni Sofiah AniqahAffiliation unknown1
Haniyya HaniyyaAffiliation unknown1
Phitsanu PinmaneeAffiliation unknown1
Kanokarn KocharinAffiliation unknown1
Duriya CantasinghAffiliation unknown1
Thidarat NimchuaAffiliation unknown1
Maimunah MaimunahAffiliation unknown1
Tri Yudani Mardining RarasAffiliation unknown1
Mauludy Jutta AjrullahAffiliation unknown1
Ellen Fenix GunawanAffiliation unknown1
Nadya Shafa PramestiAffiliation unknown1
Sumarno Reto PrawiroAffiliation unknown1
Gabriela Christy SabbthiniAffiliation unknown1
Joko Pebrianto TrinugrohoAffiliation unknown1
Muhammad AliAffiliation unknown1
Kamran MehdiAffiliation unknown1
Obaid-ur-Rahman AbidAffiliation unknown1
Saqib KhanAffiliation unknown1
Muhammad Ikhlas AbdjanAffiliation unknown1
Aleesha AleeshaAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en">Direct Cloning of a Xylanase Gene from Pawan-Riau Hot Spring</dc:title>
	<dc:creator xml:lang="en">HELIANTI, IS</dc:creator>
	<dc:description xml:lang="en">A functional gene containing an Open Reading Frame (ORF) encoding a ?-1, 4-endoxylanase glycosyl hydrolase family 11 was cloned directly using metagenomic PCR-cloning method from Pawan Hot Spring sample in Riau. The gene consisted of 642 nucleotides, encoded for 213 amino acids. The amino acid sequence analysis using BLAST showed that the gene has high homology (93%) with xylanase gene from Bacillus subtilis. The gene showed its function when it was subcloned into an expression vector and overexpressed in E. coli. The crude extract of the recombinant enzyme had activity for 170 U/ml at 50 oC. The result of this work showed that metagenomic approach was a powerful short cut method to obtain recombinant biocatalyst that was useful for industrial application. Key words: ?-1, 4-endoxylanase, metagenomic DNA, Pawan-Riau hot-spring </dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2009-12-02</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://journal.ipb.ac.id/hayati/article/view/239</dc:identifier>
	<dc:identifier>10.4308/hjb.14.2.54</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 14 No. 2 (2007): June 2007; 54</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://journal.ipb.ac.id/hayati/article/view/239/105</dc:relation>
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