Biological control of bacterial wilt pathogen (Ralstonia solanacearum) of tomato using endophytic bacteria is one of the alternative control methods to support sustainable agriculture. This study was conducted to select and characterize endophytic bacteria isolated from healthy tomato stems and to test their ability to promote plant growth and suppress bacterial wilt disease. Among 49 isolates successfully isolated, 41 were non-plant pathogenic. Green house test on six selected isolates based on antagonistic effect on R. solanacearum or ability to suppress R. solanacearum population in dual culture assays obtained BC4 and BL10 isolates as promising biocontrol agents. At six weeks after transplanting, plants treated with BC4 isolate showed significantly lower disease incidence (33%) than that of control (83%). Plants height was not significantly affected by endophytic bacterial treatments. Based on 16S rRNA sequence, BC4 isolate had 97% similarity with Staphylococcus epidermidis (accession number EU834240.1), while isolate BL10 had 98% similarity with Bacillus amyloliquefaciens strain JK-SD002 (accession number AB547229.1).

NAWANGSIH, ABDJAD ASIH; DAMAYANTI, IKA; WIYONO, SURYO; KARTIKA, JUANG GEMA

doi:10.4308/hjb.18.2.66

Journal SINTA 1

Biological control of bacterial wilt pathogen (Ralstonia solanacearum) of tomato using endophytic bacteria is one of the alternative control methods to support sustainable agriculture. This study was conducted to select and characterize endophytic bacteria isolated from healthy tomato stems and to test their ability to promote plant growth and suppress bacterial wilt disease. Among 49 isolates successfully isolated, 41 were non-plant pathogenic. Green house test on six selected isolates based on antagonistic effect on R. solanacearum or ability to suppress R. solanacearum population in dual culture assays obtained BC4 and BL10 isolates as promising biocontrol agents. At six weeks after transplanting, plants treated with BC4 isolate showed significantly lower disease incidence (33%) than that of control (83%). Plants height was not significantly affected by endophytic bacterial treatments. Based on 16S rRNA sequence, BC4 isolate had 97% similarity with Staphylococcus epidermidis (accession number EU834240.1), while isolate BL10 had 98% similarity with Bacillus amyloliquefaciens strain JK-SD002 (accession number AB547229.1).

HAYATI Journal of Biosciences · Vol. 18 No. 2 (2011)

DOI: 10.4308/hjb.18.2.66
ISSN: 2086-4094, 1978-3019
Published: 2011-10-13
Publisher: Bogor Agricultural University, Indonesia
Language: eng

Abstract

Biological control of bacterial wilt pathogen (Ralstonia solanacearum) of tomato using endophytic bacteria is one of the alternative control methods to support sustainable agriculture. This study was conducted to select and characterize endophytic bacteria isolated from healthy tomato stems and to test their ability to promote plant growth and suppress bacterial wilt disease. Among 49 isolates successfully isolated, 41 were non-plant pathogenic. Green house test on six selected isolates based on antagonistic effect on R. solanacearum or ability to suppress R. solanacearum population in dual culture assays obtained BC4 and BL10 isolates as promising biocontrol agents. At six weeks after transplanting, plants treated with BC4 isolate showed significantly lower disease incidence (33%) than that of control (83%). Plants height was not significantly affected by endophytic bacterial treatments. Based on 16S rRNA sequence, BC4 isolate had 97% similarity with Staphylococcus epidermidis (accession number EU834240.1), while isolate BL10 had 98% similarity with Bacillus amyloliquefaciens strain JK-SD002 (accession number AB547229.1).

Keywords

Collaboration network of the authors

40 authors · 124 co-authorship links · 4 across different organisations · showing the 40 most connected

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IPB University
Institut Teknologi Sepuluh Nopember
State University of Jakarta
Indonesia University of Education
Cenderawasih University
Indonesian Institute of Sciences
Affiliation unknown

Different organisations
Same organisation
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Co-author	Organisation	Joint articles
Efi Toding Tondok	Affiliation unknown	4
Abdul Munif	Institut Teknologi Sepuluh Nopember	4
Aris Tri Wahyudi	IPB University	4
Widodo Widodo	State University of Jakarta	3
Ida Parida	Affiliation unknown	3
Giyanto	Affiliation unknown	2
Sobir Sobir	Affiliation unknown	2
Sri Hendrastuti Hidayat	IPB University	2
Yulin Lestari	Affiliation unknown	2
Ely Rudyatmi	Affiliation unknown	1
Heri Harti	Affiliation unknown	1
Stephanie Stephanie	Affiliation unknown	1
Utut Widyastuti	Affiliation unknown	1
Lukman Hakim	Institut Teknologi Sepuluh Nopember	1
Didi Sukyadi	Affiliation unknown	1
Fuad Abdul Hamied	Affiliation unknown	1
Ari Arifin Danuwijaya	Affiliation unknown	1
Firly Asyifa	Affiliation unknown	1
Ari Susilowati	Affiliation unknown	1
Antonius Suwanto	Affiliation unknown	1
Wawan Setiawan	Indonesia University of Education	1
Idris Idris	Affiliation unknown	1
Masrukhin Masrukhin	Affiliation unknown	1
I Made Sudiana	Affiliation unknown	1
Atit Kanti	Affiliation unknown	1
Toga Pangihotan Napitupulu	Affiliation unknown	1
I Nyoman Sumerta	Affiliation unknown	1
Daawia Daawia	Cenderawasih University	1
Ni Putu Sri Asih	Indonesian Institute of Sciences	1
krisantini krisantini	Affiliation unknown	1
Megayani Sri Rahayu	Affiliation unknown	1
Deden D. Matra	Affiliation unknown	1
Bambang Suhartawan	Affiliation unknown	1
Lisa Marianah	Affiliation unknown	1
Kikin Hamzah Mutaqin	IPB University	1
Siti Halida Hamzah-FZ	Affiliation unknown	1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.

Original metadata (oai_dc)

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