Molecular marker is useful in the development of testicular cells transplantation for detecting donor-derived germ cells in the recipient gonad. In this study, a giant gourami (Osphronemus goramy) vasa-like gene (GgVLG) was cloned and characterized for use as a molecular marker for germ cells in this species. Nucleotide sequence analysis revealed that GgVLG comprises 2,340 bps with an open reading frame of 1,962 bps encoding 653 amino acids. The deduced amino acid sequence contained 17 arginine-glycine or arginine-glycine-glycine motifs and eight conserved motifs belonging to the DEAD-box protein family. The GgVLG sequence showed high similarity to Drosophila vasa, common carp vasa homolog and tilapia vasa homolog for 66.2, 85.9, and 90.7%, respectively. In adult tissues, the GgVLG transcripts were specifically detected in ovary and testis. In situ hybridization analysis showed that GgVLG mRNA was detected in oocytes of the ovary and spermatogonia of the testis. There was no signal detected in the spermatocytes, spermatids and other gonadal somatic cells. Thus, consensus sequences, specific localization of GgVLG mRNA in the germ cells, amino acid sequence similarity and phylogenic analysis all suggest that GgVLG is the giant gourami vasa-like gene. Further, GgVLG can be used as a molecular marker for giant gourami germ cells.

ALIMUDDIN,.; ANDRIYANI, IRMA; JUNIOR, MUHAMMAD ZAIRIN; ARFAH, HARTON; OCTAVERA, ANNA; CARMAN, ODANG; YOSHIZAKI, GORO

doi:10.4308/hjb.18.3.135

Journal SINTA 1

Molecular marker is useful in the development of testicular cells transplantation for detecting donor-derived germ cells in the recipient gonad. In this study, a giant gourami (Osphronemus goramy) vasa-like gene (GgVLG) was cloned and characterized for use as a molecular marker for germ cells in this species. Nucleotide sequence analysis revealed that GgVLG comprises 2,340 bps with an open reading frame of 1,962 bps encoding 653 amino acids. The deduced amino acid sequence contained 17 arginine-glycine or arginine-glycine-glycine motifs and eight conserved motifs belonging to the DEAD-box protein family. The GgVLG sequence showed high similarity to Drosophila vasa, common carp vasa homolog and tilapia vasa homolog for 66.2, 85.9, and 90.7%, respectively. In adult tissues, the GgVLG transcripts were specifically detected in ovary and testis. In situ hybridization analysis showed that GgVLG mRNA was detected in oocytes of the ovary and spermatogonia of the testis. There was no signal detected in the spermatocytes, spermatids and other gonadal somatic cells. Thus, consensus sequences, specific localization of GgVLG mRNA in the germ cells, amino acid sequence similarity and phylogenic analysis all suggest that GgVLG is the giant gourami vasa-like gene. Further, GgVLG can be used as a molecular marker for giant gourami germ cells.

HAYATI Journal of Biosciences · Vol. 18 No. 3 (2011)

DOI: 10.4308/hjb.18.3.135
ISSN: 2086-4094, 1978-3019
Published: 2011-12-22
Publisher: Bogor Agricultural University, Indonesia
Language: eng

Abstract

Molecular marker is useful in the development of testicular cells transplantation for detecting donor-derived germ cells in the recipient gonad. In this study, a giant gourami (Osphronemus goramy) vasa-like gene (GgVLG) was cloned and characterized for use as a molecular marker for germ cells in this species. Nucleotide sequence analysis revealed that GgVLG comprises 2,340 bps with an open reading frame of 1,962 bps encoding 653 amino acids. The deduced amino acid sequence contained 17 arginine-glycine or arginine-glycine-glycine motifs and eight conserved motifs belonging to the DEAD-box protein family. The GgVLG sequence showed high similarity to Drosophila vasa, common carp vasa homolog and tilapia vasa homolog for 66.2, 85.9, and 90.7%, respectively. In adult tissues, the GgVLG transcripts were specifically detected in ovary and testis. In situ hybridization analysis showed that GgVLG mRNA was detected in oocytes of the ovary and spermatogonia of the testis. There was no signal detected in the spermatocytes, spermatids and other gonadal somatic cells. Thus, consensus sequences, specific localization of GgVLG mRNA in the germ cells, amino acid sequence similarity and phylogenic analysis all suggest that GgVLG is the giant gourami vasa-like gene. Further, GgVLG can be used as a molecular marker for giant gourami germ cells.

Keywords

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IPB University
Institut des Sciences de l'Evolution de Montpellier
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Co-author	Organisation	Joint articles
Sri Nuryati	Affiliation unknown	4
Agus Oman Sudrajat	Affiliation unknown	2
Komar Sumantadinata	Affiliation unknown	2
Ratu Siti Aliah	Affiliation unknown	2
Shavika Miranti	Affiliation unknown	2
Irzal Effendi	IPB University	2
Shella Marlinda	Affiliation unknown	2
Harits Atika Ariyatna	Affiliation unknown	2
Sulasy Rohmy	Affiliation unknown	2
Mursalim Nohong	Affiliation unknown	1
Insany Fitri Nurqamar	Affiliation unknown	1
Wahda	Affiliation unknown	1
Sabir	Affiliation unknown	1
Andi Kusumawati	Affiliation unknown	1
Abdullah Sanusi	Affiliation unknown	1
Media Fitri Isma Nugraha	Affiliation unknown	1
Jean‐Christophe Avarre	Institut des Sciences de l'Evolution de Montpellier	1
Laurent Pouyaud	Institut des Sciences de l'Evolution de Montpellier	1
Kadarusman Kadarusman	Affiliation unknown	1
Muhammad Zairin	IPB University	1
Liza	Affiliation unknown	1
Amin Akbar	Affiliation unknown	1
Mochamad Syaifudin	Affiliation unknown	1
Utut Widyastuti	Affiliation unknown	1
SLAMET SUBYAKTO	Affiliation unknown	1
RUSTIDJA	Affiliation unknown	1
M. SASMITO JATI	Affiliation unknown	1
IRVAN FAIZAL	Affiliation unknown	1
GEMI TRIASTUTIK	Affiliation unknown	1
Muhammad Agus Suprayudi	Affiliation unknown	1
DARMAWAN SETIA BUDI	Affiliation unknown	1
Siti Fadilah	Affiliation unknown	1
Petrus Rani Pong-Masak	Affiliation unknown	1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.

Original metadata (oai_dc)

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