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Yeast Isolation for Bioethanol Production

HAYATI Journal of Biosciences · Vol. 19 No. 3 (2012)

DOI
10.4308/hjb.19.3.145
ISSN
2086-4094, 1978-3019
Published
2012-10-15
Publisher
Bogor Agricultural University, Indonesia
Language
eng

Abstract

We have isolated 12 yeast isolates from five different rotten fruits by using a yeast glucose chloramphenicol agar (YGCA) medium supplemented with tetracycline. From pre-screening assay, four isolates exhibited higher substrate (glucose-xylose) consumption efficiency in the reaction tube fermentation compared to Saccharomyces cerevisiae dan Saccharomyces ellipsoids as the reference strains. Based on the fermentation process in gooseneck flasks, we observed that two isolates (K and SB) showed high fermentation efficiency both in sole glucose and mixed glucose-xylose substrate. Moreover, isolates K and SB produced relatively identical level of ethanol concentration compared to the reference strains. Isolates H and MP could only produce high levels of ethanol in glucose fermentation, while only half of that amount of ethanol was detected in glucose-xylose fermentation. Isolate K and SB were identified as Pichia kudriavzeevii (100%) based on large sub unit (LSU) ribosomal DNA D1/D2 region.

Keywords

Collaboration network of the authors

40 authors · 91 co-authorship links · 19 across different organisations · showing the 40 most connected

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • IPB University
  • Universitas Sumatera Utara
  • National Research and Innovation Agency
  • Indonesian Center for Agricultural Biotechnology and Genetic Resource Research and Development
  • Sriwijaya University
  • University of Göttingen
  • Universiti Putra Malaysia
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Rika Indri AstutiIPB University4
Antonius SuwantoIPB University3
Aris Tri WahyudiIPB University3
Dwi SuryantoUniversitas Sumatera Utara2
YopiAffiliation unknown2
Yulin LestariIPB University2
Apon Zaenal MustopaNational Research and Innovation Agency2
Dimas AndriantoIPB University2
Aprilia NaomiIPB University2
FERY MUTIAIPB University2
M. EDY SOFIYANTOIPB University2
Nur RichanaIPB University2
Vestika Iskawati Wahidul HijahIPB University2
Yaya RukayadiUniversiti Putra Malaysia2
Eka putriIPB University2
Deden SaprudinIPB University1
Prima Agung PrihandonoIPB University1
Alina AkhdiyaIndonesian Center for Agricultural Biotechnology and Genetic Resource Research and Development1
Trio HendarwinAffiliation unknown1
Ono SuparnoIPB University1
Akhmad Endang Zainal HasanIPB University1
Djumali MangunwidjajaIPB University1
Agus SetiyonoIPB University1
Kurrataa’yunIPB University1
AriandiIPB University1
Munti YuhanaIPB University1
Marini WijayantiSriwijaya University1
Martin EngelhauptUniversity of Göttingen1
Ade IrmaAffiliation unknown1
Bedah RupaedahAffiliation unknown1
Nurfadhilla RahmadhaniIPB University1
Mazidah Noer InayahIPB University1
Arina Amalia PutriIPB University1
Alfred MichaelAffiliation unknown1
Maria SugihartiAffiliation unknown1
Muhammad Fadhil FathiahIPB University1
RamlanAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en">Yeast Isolation for Bioethanol Production</dc:title>
	<dc:creator xml:lang="en">RURIANI, EKA</dc:creator>
	<dc:creator xml:lang="en">SUNARTI, TITI CANDRA</dc:creator>
	<dc:creator xml:lang="en">MERYANDINI, ANJA</dc:creator>
	<dc:subject xml:lang="en">yeast</dc:subject>
	<dc:subject xml:lang="en">glucose-xylose substrate</dc:subject>
	<dc:subject xml:lang="en">ethanol</dc:subject>
	<dc:description xml:lang="en">We have isolated 12 yeast isolates from five different rotten fruits by using a yeast glucose chloramphenicol agar (YGCA) medium supplemented with tetracycline. From pre-screening assay, four isolates exhibited higher substrate (glucose-xylose) consumption efficiency in the reaction tube fermentation compared to Saccharomyces cerevisiae dan Saccharomyces ellipsoids as the reference strains. Based on the fermentation process in gooseneck flasks, we observed that two isolates (K and SB) showed high fermentation efficiency both in sole glucose and mixed glucose-xylose substrate. Moreover, isolates K and SB produced relatively identical level of ethanol concentration compared to the reference strains. Isolates H and MP could only produce high levels of ethanol in glucose fermentation, while only half of that amount of ethanol was detected in glucose-xylose fermentation. Isolate K and SB were identified as Pichia kudriavzeevii (100%) based on large sub unit (LSU) ribosomal DNA D1/D2 region.</dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2012-10-15</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://journal.ipb.ac.id/hayati/article/view/5632</dc:identifier>
	<dc:identifier>10.4308/hjb.19.3.145</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 19 No. 3 (2012): September 2012; 145</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://journal.ipb.ac.id/hayati/article/view/5632/4271</dc:relation>
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