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Archaeal Life on Tangkuban Perahu- Sampling and Culture Growth in Indonesian Laboratories

HAYATI Journal of Biosciences · Vol. 19 No. 3 (2012)

DOI
10.4308/hjb.19.3.150
ISSN
2086-4094, 1978-3019
Published
2012-10-15
Publisher
Bogor Agricultural University, Indonesia
Language
eng

Abstract

The aim of the expedition to Tangkuban Perahu, West Java was to obtain archaeal samples from the solfatara fields located in Domas crater. This was one of the places, where scientists from the University of Regensburg Germany had formerly isolated Indonesian archaea, especially Thermoplasma and Sulfolobus species but not fully characterized. We collected five samples from mud holes with temperatures from 57 to 88 oC and pH of 1.5-2. A portion of each sample was grown at the University of Regensburg in modified Allen’s medium at 80 oC. From four out of five samples enrichment cultures were obtained, autotrophically on elemental sulphur and heterotrophically on sulfur and yeast extract; electron micrographs are presented. In the laboratories of Universitas Indonesia the isolates were cultured at 55-60 oC in order to grow tetraetherlipid synthesizing archaea, both Thermoplasmatales and Sulfolobales. Here, we succeeded to culture the same type of archaeal cells, which had been cultured in Regensburg, probably a Sulfolobus species and in Freundt’s medium, Thermoplasma species. The harvested cells are documented by phase contrast microscope equipped with a digital camera. Our next steps will be to further characterize genetically the cultured cells from Tangkuban Perahu isolates.

Keywords

Collaboration network of the authors

40 authors · 125 co-authorship links · 1 across different organisations · showing the 40 most connected

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  • University of Brawijaya
  • National Research and Innovation Agency
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Riris Istighfari JenieAffiliation unknown6
Mohamad SyaefudinAffiliation unknown4
Diah Vitri WidayantiAffiliation unknown4
Ratna Asmah SusidartiAffiliation unknown4
Neli PurwaniAffiliation unknown3
Mohamad SadikinAffiliation unknown3
RINI PUSPITANINGRUMAffiliation unknown3
RONDANG ROEMIATI SOEGIANTOAffiliation unknown3
Edy MeiyantoAffiliation unknown3
Zalinar UdinAffiliation unknown3
Dwi AstutiAffiliation unknown2
Tri Eko AgustiningrumAffiliation unknown2
Sudarwoto SudarwotoAffiliation unknown2
Nurul YuhanafiaAffiliation unknown2
Virgiawan Adi KristiantoAffiliation unknown2
Cici DarsihAffiliation unknown2
Wega TrisunaryantiAffiliation unknown2
TriyonoAffiliation unknown2
Aan KurniawanNational Research and Innovation Agency2
Syarifah NurainiAffiliation unknown2
Ani Budi AstutiUniversity of Brawijaya1
Suci AstutikUniversity of Brawijaya1
Nur Silviyah RahmiUniversity of Brawijaya1
Alifiandi Rafi MuhammadUniversity of Brawijaya1
Ulfah MaisarohUniversity of Brawijaya1
Kismi MubarokahAffiliation unknown1
Harianingsih HarianingsihAffiliation unknown1
Maharani KusumaningrumAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en">Archaeal Life on Tangkuban Perahu- Sampling and Culture Growth in Indonesian Laboratories</dc:title>
	<dc:creator xml:lang="en">HANDAYANI, SRI</dc:creator>
	<dc:creator xml:lang="en">SANTOSO, IMAN</dc:creator>
	<dc:creator xml:lang="en">FREISLEBEN, HANS-JOACHIM</dc:creator>
	<dc:creator xml:lang="en">HUBER, HARALD</dc:creator>
	<dc:creator xml:lang="en">ANDI, .</dc:creator>
	<dc:creator xml:lang="en">ARDIANSYAH, FERY</dc:creator>
	<dc:creator xml:lang="en">MULYANTO, CENMI</dc:creator>
	<dc:creator xml:lang="en">LUTHFA, ZESSINDA</dc:creator>
	<dc:creator xml:lang="en">SALEH, ROSARI</dc:creator>
	<dc:creator xml:lang="en">FREISLEBEN, SERUNI KUSUMA  UDYANINGSIH</dc:creator>
	<dc:creator xml:lang="en">WANANDI, SEPTELIA INAWATI</dc:creator>
	<dc:creator xml:lang="en">THOMM, MICHAEL</dc:creator>
	<dc:subject xml:lang="en">Archaea</dc:subject>
	<dc:subject xml:lang="en">Sulfolobus</dc:subject>
	<dc:subject xml:lang="en">Thermoplasma</dc:subject>
	<dc:subject xml:lang="en">Tangkuban Perahu</dc:subject>
	<dc:subject xml:lang="en">Indonesian volcanoes</dc:subject>
	<dc:subject xml:lang="en">tetraether lipid</dc:subject>
	<dc:description xml:lang="en">The aim of the expedition to Tangkuban Perahu, West Java was to obtain archaeal samples from the solfatara fields located in Domas crater. This was one of the places, where scientists from the University of Regensburg Germany had formerly isolated Indonesian archaea, especially Thermoplasma and Sulfolobus species but not fully characterized. We collected five samples from mud holes with temperatures from 57 to 88 oC and pH of 1.5-2. A portion of each sample was grown at the University of Regensburg in modified Allen’s medium at 80 oC. From four out of five samples enrichment cultures were obtained, autotrophically on elemental sulphur and heterotrophically on sulfur and yeast extract; electron micrographs are presented. In the laboratories of Universitas Indonesia the isolates were cultured at 55-60 oC in order to grow tetraetherlipid synthesizing archaea, both Thermoplasmatales and Sulfolobales. Here, we succeeded to culture the same type of archaeal cells, which had been cultured in Regensburg, probably a Sulfolobus species and in Freundt’s medium, Thermoplasma species. The harvested cells are documented by phase contrast microscope equipped with a digital camera. Our next steps will be to further characterize genetically the cultured cells from Tangkuban Perahu isolates.</dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2012-10-15</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://journal.ipb.ac.id/hayati/article/view/5639</dc:identifier>
	<dc:identifier>10.4308/hjb.19.3.150</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 19 No. 3 (2012): September 2012; 150</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://journal.ipb.ac.id/hayati/article/view/5639/4272</dc:relation>
</oai_dc:dc>