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Genetic Diversity of Klebsiella spp. Isolated from Tempe based on Enterobacterial Repetitive Intergenic Consensus-Polymerase Chain Reaction (ERIC-PCR)

HAYATI Journal of Biosciences · Vol. 20 No. 4 (2013) · pp. 171–176

DOI
10.4308/hjb.20.4.171-176
ISSN
2086-4094, 1978-3019
Published
2013-12-31
Publisher
Bogor Agricultural University, Indonesia
Language
eng

Abstract

Tempe is an Indonesian fermented food prepared by fermenting dehulled cooked soybeans with Rhizopus oligosporus. Many types of bacteria are also involved during tempe fermentation, and one of these is Klebsiella spp. Some isolates of K. pneumoniae produces vitamin B12 in tempe but it has also been classified as an opportunistic pathogen. For this reason Klebsiella spp. in tempe is important to be studied. The aim of this study was to investigate the genetic diversity of Klebsiella spp. from tempe employing ERIC-PCR method. Sixty-one isolates of Klebsiella have been isolated from sixteen tempe producers in Bogor, Jakarta, Malang, Tengerang, Bandung and Cianjur. 63F and 1387R primers were used to amplify 16S rDNA sequences, and 1R and 1F primers were used for ERIC analysis. The results of this research showed that sixty-one strains of Klebsiella were clustered into 17 groups. Based on ERIC-PCR analysis, isolates of Klebsiella could be grouped into different profiles which some of these groups consisted of isolates with identical ERIC-PCR profiles. Several identical ERIC-PCR profiles were found in tempe from the same producer. There was no correlation observed between genetic similarity among isolates with the origin of tempe.

Keywords

Collaboration network of the authors

40 authors · 107 co-authorship links · showing the 40 most connected

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  • IPB University
  • Institut Agama Islam Negeri Kendari
  • Affiliation unknown
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Co-authorOrganisationJoint articles
ESTI PUSPITASARIAffiliation unknown4
Iman RusmanaAffiliation unknown4
Nisa Rachmania MubarikAffiliation unknown4
Dwi SuryantoAffiliation unknown3
Anja MeryandiniAffiliation unknown3
Budi TjahjonoAffiliation unknown3
Aris Tri WahyudiIPB University3
Andreas Adhi SatyaAffiliation unknown3
Rika Indri AstutiAffiliation unknown3
Widanarni WidanarniIPB University2
AMARILA MALIKAffiliation unknown2
Yulin LestariAffiliation unknown2
Cecilia Anna SeumahuAffiliation unknown2
Dedy Duryadi SolihinAffiliation unknown2
Maria Indah PurnamasariAffiliation unknown2
Yepy Hardi RustamAffiliation unknown2
Kartini KramadibrataAffiliation unknown2
Nampiah SukarnoAffiliation unknown2
Sri ListiyowatiAffiliation unknown2
Nurul AiniInstitut Agama Islam Negeri Kendari1
WALTER ERDELENAffiliation unknown1
Yusminah HalaAffiliation unknown1
Ridwan AffandiAffiliation unknown1
MUHAMMAD ZAIRIN Jr.Affiliation unknown1
ROB HARLINGAffiliation unknown1
SumardiAffiliation unknown1
MAGGY T HENAWIDJAJAAffiliation unknown1
T RESNAWATI P URWADARIAAffiliation unknown1
NURITA TORUAN-MATHIUSAffiliation unknown1
Irang WahyunantoAffiliation unknown1
Diana E WaturangiAffiliation unknown1
ALINA AKHDIYA RUSMANAAffiliation unknown1
ROHANI CINTA BADIA GINTINGAffiliation unknown1
ARIS TJAHJOLEKSONOAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en">Genetic Diversity of Klebsiella spp. Isolated from Tempe based on Enterobacterial Repetitive Intergenic Consensus-Polymerase Chain Reaction (ERIC-PCR)</dc:title>
	<dc:creator xml:lang="en">BARUS, TATI</dc:creator>
	<dc:creator xml:lang="en">HANJAYA, IVAN</dc:creator>
	<dc:creator xml:lang="en">SADELI, JOANITA</dc:creator>
	<dc:creator xml:lang="en">LAY, BIBIANA W</dc:creator>
	<dc:creator xml:lang="en">SUWANTO, ANTONIUS</dc:creator>
	<dc:creator xml:lang="en">YULANDI, ADI</dc:creator>
	<dc:subject xml:lang="en">Tempe</dc:subject>
	<dc:subject xml:lang="en">Klebsiella</dc:subject>
	<dc:subject xml:lang="en">ERIC-PCR</dc:subject>
	<dc:subject xml:lang="en">vitamin B12</dc:subject>
	<dc:description xml:lang="en">Tempe is an Indonesian fermented food prepared by fermenting dehulled cooked soybeans with Rhizopus oligosporus. Many types of bacteria are also involved during tempe fermentation, and one of these is Klebsiella spp.  Some isolates of K.  pneumoniae produces vitamin B12 in tempe but it has also been classified as an opportunistic pathogen. For this reason Klebsiella spp. in tempe is important to be studied. The aim of this study was to investigate the genetic diversity of Klebsiella spp. from tempe employing ERIC-PCR method. Sixty-one isolates of Klebsiella have been isolated from sixteen tempe producers  in Bogor, Jakarta, Malang, Tengerang, Bandung and Cianjur. 63F and 1387R primers were used to amplify 16S rDNA sequences, and 1R and 1F primers were used for ERIC analysis. The results of this research showed that sixty-one strains of Klebsiella were clustered into 17 groups. Based on ERIC-PCR analysis, isolates of Klebsiella could be grouped into different profiles which some of these groups consisted of isolates with identical ERIC-PCR profiles. Several identical ERIC-PCR profiles were found in tempe from the same producer. There was no correlation observed between genetic similarity  among isolates with the origin of tempe.</dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2013-12-31</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://journal.ipb.ac.id/hayati/article/view/7320</dc:identifier>
	<dc:identifier>10.4308/hjb.20.4.171-176</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 20 No. 4 (2013): December 2013; 171-176</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://journal.ipb.ac.id/hayati/article/view/7320/pdf_1</dc:relation>
</oai_dc:dc>