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Characterization of Bacteriophage Specific to Bacillus pumilus from Ciapus River in Bogor, West Java, Indonesia

HAYATI Journal of Biosciences · Vol. 22 No. 1 (2015)

DOI
10.4308/hjb.22.1.27
ISSN
2086-4094, 1978-3019
Published
2015-01-02
Publisher
Bogor Agricultural University, Indonesia
Language
eng

Abstract

Bacillus pumilus is a spore-forming bacteria that is rod-shaped, gram positive, and aerobic. B. pumilus produced pumilacidins, known to have toxic effects on epithelial cells. Antibiotics were usually used to treat the disease caused by bacteria. Antibiotic typing test of B. pumilus indigenous from sewage water showed that this isolate was resistant to ampicillin and clindamycin. An alternative way was by application of bacteriophages as biocontrol agents to reduce B. pumilus in environment. The aim of this study were to isolate and characterize B. pumilus bacteriophage isolated from Ciapus River in Bogor, West Java. Bacteriophages infecting B. pumilus were isolated from river water using the double agar overlay method. Phages were defined by plaque morphology, structure, host range, and characteristic of molecular weight protein phage. Phage FBa1, FBa2, and FBa3 had narrow host range and they were specific for infecting B. pumilus. Electron microscope observation showed that phage FBa1 had icosahedral head without tail (166.67 nm in diameter), so it is called phage-like particles. Characterization of phage FBa1 by SDS-PAGE showed five proteins band. Molecular weight of FBa1 proteins was 70.9, 54.9, 33.8, 28.3, and 21.4 kDa.

Keywords

Collaboration network of the authors

40 authors · 140 co-authorship links · showing the 40 most connected

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  • IPB University
  • Universitas Papua
  • Sunan Gunung Djati State Islamic University Bandung
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
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Co-authorOrganisationJoint articles
Nisa Rachmania MubarikAffiliation unknown6
Antonius SuwantoAffiliation unknown4
Rika Indri AstutiAffiliation unknown4
Yulin LestariAffiliation unknown3
Liga InsaniAffiliation unknown2
Diah Ayu Satyari UtamiAffiliation unknown2
Sipriyadi SipriyadiAffiliation unknown2
Risky Hadi WibowoAffiliation unknown2
Maggy Thenawidjaya SuhartonoAffiliation unknown2
Hamim HamimAffiliation unknown2
BAMBANG SURYOBROTOAffiliation unknown2
Alex HartanaAffiliation unknown2
Cecilia Anna SeumahuAffiliation unknown2
Dedy Duryadi SolihinAffiliation unknown2
Alina AkhdiyaAffiliation unknown2
Aris Tri WahyudiIPB University2
IlhamAffiliation unknown1
Annisa Khairani ArasAffiliation unknown1
Mohsan AbroriAffiliation unknown1
I Nyoman SudiarsaAffiliation unknown1
Arie KiswantoAffiliation unknown1
Andina Chairun NisaAffiliation unknown1
Diklawati JatayuAffiliation unknown1
Amiqatul FikriyahAffiliation unknown1
Muhammad Chaidir UnduAffiliation unknown1
Dian HendrayantiAffiliation unknown1
Dwi Andreas SantosaAffiliation unknown1
ALINA AKHADIYAAffiliation unknown1
TETRI WIDIYANIAffiliation unknown1
Elda Irma Jeanne Joice KawulurUniversitas Papua1
Dewi SartikaSunan Gunung Djati State Islamic University Bandung1
MIRNAWATI SUDARWANTOAffiliation unknown1
DONDIN SAJUTHIAffiliation unknown1
SUSAN SOKAAffiliation unknown1
DIAH ISKANDRIATIAffiliation unknown1
KATHARINA JESSICAAffiliation unknown1
. MahyarudinAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en">Characterization of Bacteriophage Specific to Bacillus pumilus from Ciapus River in Bogor, West Java, Indonesia</dc:title>
	<dc:creator xml:lang="en">KUSMIATUN, ANIK</dc:creator>
	<dc:creator xml:lang="en">RUSMANA, IMAN</dc:creator>
	<dc:creator xml:lang="en">BUDIARTI, SRI</dc:creator>
	<dc:subject xml:lang="en">Bacillus pumilus</dc:subject>
	<dc:subject xml:lang="en">bacteriophage</dc:subject>
	<dc:subject xml:lang="en">food poisoning</dc:subject>
	<dc:description xml:lang="en">Bacillus pumilus is a spore-forming bacteria that is rod-shaped, gram positive, and aerobic. B. pumilus produced pumilacidins, known to have toxic effects on epithelial cells. Antibiotics were usually used to treat the disease caused by bacteria. Antibiotic typing test of B. pumilus indigenous from sewage water showed that this isolate was resistant to ampicillin and clindamycin. An alternative way was by application of bacteriophages as biocontrol agents to reduce B. pumilus in environment. The aim of this study were to isolate and characterize B. pumilus bacteriophage isolated from Ciapus River in Bogor, West Java. Bacteriophages infecting B. pumilus were isolated from river water using the double agar overlay method. Phages were defined by plaque morphology, structure, host range, and characteristic of molecular weight protein phage. Phage FBa1, FBa2, and FBa3 had narrow host range and they were specific for infecting B. pumilus. Electron microscope observation showed that phage FBa1 had icosahedral head without tail (166.67 nm in diameter), so it is called phage-like particles. Characterization of phage FBa1 by SDS-PAGE showed five proteins band. Molecular weight of FBa1 proteins was 70.9, 54.9, 33.8, 28.3, and 21.4 kDa.</dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2015-01-02</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://journal.ipb.ac.id/hayati/article/view/9217</dc:identifier>
	<dc:identifier>10.4308/hjb.22.1.27</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 22 No. 1 (2015): January 2015; 27</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://journal.ipb.ac.id/hayati/article/view/9217/pdf</dc:relation>
</oai_dc:dc>