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Construction of RNA Interference Vector to Silence Aluminum Tolerance Gene Candidate in Rice cv Hawara Bunar

HAYATI Journal of Biosciences · Vol. 23 No. 2 (2016)

DOI
10.4308/hjb.23.2.79
ISSN
2086-4094, 1978-3019
Published
2016-11-10
Publisher
Bogor Agricultural University, Indonesia
Language
eng

Abstract

One of the aluminum (Al) tolerance gene candidates, namely B11 gene, has been successfully isolated from Al-tolerant rice cv Hawara Bunar. However, the role of the gene in Al tolerance in rice has not been known. RNA interference (RNAi) technique is an effective tool to examine the biological function of the target gene in plant. The objective of the research was to construct RNAi recombinant vector carrying untranslated region of the B11 gene. RNAi recombinant vector carrying 195 bp sized 3′UTR_B11 fragment as a double-stranded RNA (dsRNA) trigger has been successfully constructed using GATEWAY™ cloning technology, pENTR™/D-TOPO® as a shuttle vector, and pANDA vector as a destination vector. RNAi construct was successfully introduced into Agrobacterium tumefaciens AgL0, and has been infected to rice cv Hawara Bunar. Analysis of putative transgenic rice showed eight of 20 plants were transgenic carrying the B11-RNAi construct.

Keywords

Collaboration network of the authors

40 authors · 98 co-authorship links · 1 across different organisations · showing the 40 most connected

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  • National Research and Innovation Agency
  • University of Darussalam Gontor
  • Institut Agama Islam Negeri Kendari
  • Universitas Rokania
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
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Co-authorOrganisationJoint articles
Suharsono SuharsonoAffiliation unknown6
ROHANI CINTA BADIA GINTINGAffiliation unknown3
Ence Darmo Jaya SupenaAffiliation unknown2
Nampiah SukarnoAffiliation unknown2
LATIFAH KOSIM DARUSMANAffiliation unknown2
SIHEGIKO KANAYAAffiliation unknown2
M. MiftahudinAffiliation unknown2
Triadiati TriadiatiAffiliation unknown2
Hamim HamimAffiliation unknown2
Adi Nur CahyonoAffiliation unknown1
Himmah RustiamiNational Research and Innovation Agency1
Tatik ChikmawatiAffiliation unknown1
I Putu Gede P. DamayantoAffiliation unknown1
Asep MuhyiddinAffiliation unknown1
AlimuddinAffiliation unknown1
Mochamad SyaifudinAffiliation unknown1
Agus Oman SudrajatAffiliation unknown1
Komar SumantadinataAffiliation unknown1
Ratu Siti AliahAffiliation unknown1
Niken TrisnaningrumUniversity of Darussalam Gontor1
LULUT DWI SULISTYANINGSIHAffiliation unknown1
Stephanie StephanieAffiliation unknown1
Suryo WiyonoAffiliation unknown1
Alimuddin AlimuddinAffiliation unknown1
Tri HandayaniAffiliation unknown1
Andi ParenrengiAffiliation unknown1
Emma SuryatiAffiliation unknown1
Erina SulistianiAffiliation unknown1
Antonius SuwantoAffiliation unknown1
Nurlaela NurlaelaInstitut Agama Islam Negeri Kendari1
Juliarni JuliarniUniversitas Rokania1
SuharsonoNational Research and Innovation Agency1
Didy SopandieAffiliation unknown1
Radite TistamaAffiliation unknown1
AKIHO YOKOTAAffiliation unknown1
KINYA AKASHIAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en">Construction of RNA Interference Vector to Silence Aluminum Tolerance Gene Candidate in Rice cv Hawara Bunar</dc:title>
	<dc:creator xml:lang="en">Wahyuningtyas, Windarti</dc:creator>
	<dc:creator xml:lang="en">Miftahudin, Miftahudin</dc:creator>
	<dc:creator xml:lang="en">Widyastuti, Utut</dc:creator>
	<dc:creator xml:lang="en">Tjahjoleksono, Aris</dc:creator>
	<dc:subject xml:lang="en">3′UTR_B11</dc:subject>
	<dc:subject xml:lang="en">Al tolerant</dc:subject>
	<dc:subject xml:lang="en">rice</dc:subject>
	<dc:subject xml:lang="en">RNAi vector</dc:subject>
	<dc:description xml:lang="en">One of the aluminum (Al) tolerance gene candidates, namely B11 gene, has been successfully isolated from Al-tolerant rice cv Hawara Bunar. However, the role of the gene in Al tolerance in rice has not been known. RNA interference (RNAi) technique is an effective tool to examine the biological function of the target gene in plant. The objective of the research was to construct RNAi recombinant vector carrying untranslated region of the B11 gene. RNAi recombinant vector carrying 195 bp sized 3′UTR_B11 fragment as a double-stranded RNA (dsRNA) trigger has been successfully constructed using GATEWAY™ cloning technology, pENTR™/D-TOPO® as a shuttle vector, and pANDA vector as a destination vector. RNAi construct was successfully introduced into Agrobacterium tumefaciens AgL0, and has been infected to rice cv Hawara Bunar. Analysis of putative transgenic rice showed eight of 20 plants were transgenic carrying the B11-RNAi construct.</dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2016-11-10</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://journal.ipb.ac.id/hayati/article/view/13940</dc:identifier>
	<dc:identifier>10.4308/hjb.23.2.79</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 23 No. 2 (2016): April 2016; 79</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://journal.ipb.ac.id/hayati/article/view/13940/10451</dc:relation>
</oai_dc:dc>