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Diversity of Protease-Producing Bacillus spp. From Fresh Indonesian Tempeh Based on 16S rRNA Gene Sequence

HAYATI Journal of Biosciences · Vol. 24 No. 1 (2018)

DOI
10.4308/hjb.24.1.35
ISSN
2086-4094, 1978-3019
Published
2018-03-23
Publisher
Bogor Agricultural University, Indonesia
Language
eng

Abstract

Tempeh is a type of traditional fermented food in Indonesia. The fermentation can be performed by Rhizopus microsporus as a main microorganism. However, Bacillus spp. is found in abundance in tempeh production. Nevertheless, information regarding the diversity of Bacillus spp. in tempeh production has not been reported yet. Therefore, the aim of this investigation was to study the genetic diversity of Bacillus spp. in tempeh production based on the 16S ribosomal RNA sequence. In this study, about 22 of 24 fresh tempeh from Jakarta, Bogor, and Tangerang were used. A total of 52 protease-producing Bacillus spp. isolates were obtained. Based on 16S ribosomal RNA results, all 52 isolates were identified to be similar to B. pumilus, B. subtilis, B. megaterium, B. licheniformis, B. cereus, B. thuringiensis, B. amyloliquefaciens, Brevibacillus brevis, and Bacillus sp. All the identified isolates were divided into two large clusters: 1) a cluster of B. cereus, B. thuringiensis, Bacillus sp., and B. brevis and 2) a cluster of B. pumilus, B. subtilis, B. megaterium, B. licheniformis, and B. amyloliquefaciens. Information about the Bacillus spp. role in determining the quality of tempeh has not been reported and this is a preliminary study of Bacillus spp. from tempeh.

Keywords

Collaboration network of the authors

40 authors · 96 co-authorship links · showing the 40 most connected

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  • IPB University
  • Institut Agama Islam Negeri Kendari
  • Affiliation unknown
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Co-authorOrganisationJoint articles
ESTI PUSPITASARIAffiliation unknown4
Iman RusmanaAffiliation unknown4
Nisa Rachmania MubarikAffiliation unknown4
Dwi SuryantoAffiliation unknown3
Anja MeryandiniAffiliation unknown3
Budi TjahjonoAffiliation unknown3
Aris Tri WahyudiIPB University3
Andreas Adhi SatyaAffiliation unknown3
Adi YulandiAffiliation unknown3
Widanarni WidanarniIPB University2
AMARILA MALIKAffiliation unknown2
Yulin LestariAffiliation unknown2
Cecilia Anna SeumahuAffiliation unknown2
Dedy Duryadi SolihinAffiliation unknown2
Maria Indah PurnamasariAffiliation unknown2
Rika Indri AstutiAffiliation unknown2
Yepy Hardi RustamAffiliation unknown2
SUSAN SOKAAffiliation unknown2
Ratni Kumala SariAffiliation unknown1
Syauti Alisa RahmaAffiliation unknown1
Nurhusna NurhusnaAffiliation unknown1
Indah MawartiAffiliation unknown1
Evita AnggereiniAffiliation unknown1
Risnita RisnitaAffiliation unknown1
Nurul AiniInstitut Agama Islam Negeri Kendari1
WALTER ERDELENAffiliation unknown1
Yusminah HalaAffiliation unknown1
Ridwan AffandiAffiliation unknown1
MUHAMMAD ZAIRIN Jr.Affiliation unknown1
ROB HARLINGAffiliation unknown1
SumardiAffiliation unknown1
MAGGY T HENAWIDJAJAAffiliation unknown1
T RESNAWATI P URWADARIAAffiliation unknown1
ALINA AKHDIYA RUSMANAAffiliation unknown1
ROHANI CINTA BADIA GINTINGAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en">Diversity of Protease-Producing Bacillus spp. From Fresh Indonesian Tempeh Based on 16S rRNA Gene Sequence</dc:title>
	<dc:creator xml:lang="en">Barus, Tati</dc:creator>
	<dc:creator xml:lang="en">Wati, Linda</dc:creator>
	<dc:creator xml:lang="en">Melani, .</dc:creator>
	<dc:creator xml:lang="en">Suwanto, Antonius</dc:creator>
	<dc:creator xml:lang="en">Yogiara, .</dc:creator>
	<dc:subject xml:lang="en">Bacillus spp</dc:subject>
	<dc:subject xml:lang="en">protease</dc:subject>
	<dc:subject xml:lang="en">Rhizopus microsporus</dc:subject>
	<dc:subject xml:lang="en">tempeh</dc:subject>
	<dc:description xml:lang="en">Tempeh is a type of traditional fermented food in Indonesia. The fermentation can be performed by Rhizopus microsporus as a main microorganism. However, Bacillus spp. is found in abundance in tempeh production. Nevertheless, information regarding the diversity of Bacillus spp. in tempeh production has not been reported yet. Therefore, the aim of this investigation was to study the genetic diversity of Bacillus spp. in tempeh production based on the 16S ribosomal RNA sequence. In this study, about 22 of 24 fresh tempeh from Jakarta, Bogor, and Tangerang were used. A total of 52 protease-producing Bacillus spp. isolates were obtained. Based on 16S ribosomal RNA results, all 52 isolates were identified to be similar to B. pumilus, B. subtilis, B. megaterium, B. licheniformis, B. cereus, B. thuringiensis, B. amyloliquefaciens, Brevibacillus brevis, and Bacillus sp. All the identified isolates were divided into two large clusters: 1) a cluster of B. cereus, B. thuringiensis, Bacillus sp., and B. brevis and 2) a cluster of B. pumilus, B. subtilis, B. megaterium, B. licheniformis, and B. amyloliquefaciens. Information about the Bacillus spp. role in determining the quality of tempeh has not been reported and this is a preliminary study of Bacillus spp. from tempeh.</dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2018-03-23</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://journal.ipb.ac.id/hayati/article/view/20765</dc:identifier>
	<dc:identifier>10.4308/hjb.24.1.35</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 24 No. 1 (2017): January 2017; 35</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://journal.ipb.ac.id/hayati/article/view/20765/14237</dc:relation>
</oai_dc:dc>