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Expression of Hypoxia-Inducible Factor-1α and Myoglobin in Rat Heart as Adaptive Response to Intermittent Hypobaric Hypoxia Exposure

HAYATI Journal of Biosciences · Vol. 24 No. 3 (2018)

DOI
10.4308/hjb.24.3.131
ISSN
2086-4094, 1978-3019
Published
2018-03-26
Publisher
Bogor Agricultural University, Indonesia
Language
eng

Abstract

The aim of this study was to investigate the influence of intermittent hypobaric hypoxia on the expression hypoxia adaptation proteins, namely hypoxia inducibla factor-1a (HIF-1a) and myoglobin (Mb). Twenty five male Sprague-Dawley rats were exposed to intermittent hypobaric hypoxia in a hypobaric chamber in Indonesian Air Force Institute of Aviation Medicine, for 49.5 minutes at various low pressure, 1 week interval for 4 times (day 1, 8, 15 and 22). HIF-1α and Mb protein were measured with ELISA. mRNA expression of Mb was measured with one step real time RT-PCR. HIF-1α protein levels increased after induction of hypobaric hypoxia and continues to decrease after induction of intermittent hypobaric hypoxia 3 times (ANOVA, p = 0.0437). mRNA expression and protein of Mb increased after induction of hypobaric hypoxia and continues to decrease after induction of intermittent hypobaric hypoxia 3 times (ANOVA, p = 0.0283; 0.0170), and both are strongly correlated (Pearson, r = 0.6307). The heart of rats adapted to intermittent hypoxia conditions by upregulation the expression of HIF-1a and myoglobin and then both return to normal level.

Keywords

Collaboration network of the authors

40 authors · 114 co-authorship links · showing the 40 most connected

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Co-authorOrganisationJoint articles
RINI PUSPITANINGRUMAffiliation unknown4
SEPTELIA INAWATI WANANDIAffiliation unknown3
RONDANG ROEMIATI SOEGIANTOAffiliation unknown3
DWIRINI RETNO GUNARTIAffiliation unknown3
Wardaya WardayaAffiliation unknown3
Dewi SukmawatiAffiliation unknown2
Puspita Eka WuyungAffiliation unknown2
Sri Nurbowo ArdiAffiliation unknown2
Muhammad Arza PutraAffiliation unknown2
Wiwien Heru WiyonoAffiliation unknown2
Toar Jean Maurice LalisangAffiliation unknown1
Ridho Ardhi SyaifulAffiliation unknown1
Novi Silvia HardianyAffiliation unknown1
Yefta MoenadjatAffiliation unknown1
DARYL ROBERT WILLIAMSAffiliation unknown1
ANDREW ROBERT COSSINSAffiliation unknown1
. SUBANDRATEAffiliation unknown1
Hanifah RahmiAffiliation unknown1
RusdiAffiliation unknown1
KURNIA NUZTIR MANTOLINIAffiliation unknown1
WAHONO SUMARYONOAffiliation unknown1
AMARILA MALIKAffiliation unknown1
Trismilah MargonoAffiliation unknown1
Ani Retno PrijantiAffiliation unknown1
Abdul Halim SadikinAffiliation unknown1
Sri Widia Azraki JusmanAffiliation unknown1
Hermansyah HermansyahAffiliation unknown1
Mgs. M. Irsan SalehAffiliation unknown1
Subandrate SubandrateAffiliation unknown1
Zen HafyAffiliation unknown1
Yudianita KesumaAffiliation unknown1
K KartiniAffiliation unknown1
Ahmad A JusufAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Expression of Hypoxia-Inducible Factor-1α and Myoglobin in Rat Heart as Adaptive Response to Intermittent Hypobaric Hypoxia Exposure</dc:title>
	<dc:creator xml:lang="en">Herawati, Margaretha</dc:creator>
	<dc:creator xml:lang="en">Wardaya, .</dc:creator>
	<dc:creator xml:lang="en">Mulyawan, Wawan</dc:creator>
	<dc:creator xml:lang="en">Farhan, Fanny Septiani</dc:creator>
	<dc:creator xml:lang="en">Ferdinal, Frans</dc:creator>
	<dc:creator xml:lang="en">Jusman, Sri Widia A.</dc:creator>
	<dc:creator xml:lang="en">Sadikin, Mohamad</dc:creator>
	<dc:subject xml:lang="en">intermittent hypobaric hypoxia</dc:subject>
	<dc:subject xml:lang="en">HIF-1a</dc:subject>
	<dc:subject xml:lang="en">Mb</dc:subject>
	<dc:description xml:lang="en">The aim of this study was to investigate the influence of intermittent hypobaric hypoxia on the expression hypoxia adaptation proteins, namely hypoxia inducibla factor-1a (HIF-1a) and myoglobin (Mb). Twenty five male Sprague-Dawley rats were exposed to intermittent hypobaric hypoxia in a hypobaric chamber in Indonesian Air Force Institute of Aviation Medicine, for 49.5 minutes at various low pressure, 1 week interval for 4 times (day 1, 8, 15 and 22). HIF-1α and Mb protein were measured with ELISA. mRNA expression of Mb was measured with one step real time RT-PCR. HIF-1α protein levels increased after induction of hypobaric hypoxia and continues to decrease after induction of intermittent hypobaric hypoxia 3 times (ANOVA, p = 0.0437). mRNA expression and protein of Mb increased after induction of hypobaric hypoxia and continues to decrease after induction of intermittent hypobaric hypoxia 3 times (ANOVA, p = 0.0283; 0.0170), and both are strongly correlated (Pearson, r = 0.6307). The heart of rats adapted to intermittent hypoxia conditions by upregulation the expression of HIF-1a and myoglobin and then both return to normal level.</dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2018-03-26</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://journal.ipb.ac.id/hayati/article/view/20807</dc:identifier>
	<dc:identifier>10.4308/hjb.24.3.131</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 24 No. 3 (2017): July 2017; 131</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://journal.ipb.ac.id/hayati/article/view/20807/14253</dc:relation>
</oai_dc:dc>