Journal SINTA 1
Effect of Ethylene Glycol on Structural Integrity at Each Stage of Preantral Follicle Development Post Vitrification of Rat Ovary-Histological Analysis
HAYATI Journal of Biosciences · Vol. 28 No. 4 (2021) · pp. 304–311
- ISSN
- 2086-4094, 1978-3019
- Published
- 2021-09-29
- Publisher
- Bogor Agricultural University, Indonesia
- Language
- eng
Abstract
The structure of follicular tissue affects the ability to maintain the structural integrity of follicles against cryoinjury post-vitrification. Histological analysis was conducted on the structural integrity of each stage of preantral follicles post-vitrification using 7.5% and 15.0% doses of ethylene glycol (EG), and ovarian sections with HE staining were observed using an Olympus CX21 microscope connected to Optilab 3.0 lens and Image Raster software. Analysis was conducted on the ovarian cortex in the tracing line area using polygon measure tools to obtain follicle density (follicles/mm2) and follicle index (%) data. The result showed that the EG group 7.5% (KP1) increased follicle density compared to the vitrified group (KKV) in primordial (15.83±1.77) and primary (22.94±8.51) stages. Meanwhile, KP2 (EG 15%) was in primordial (41.92±6.45), primary (11.69±1.95), secondary (33.48±3.63), and tertiary (5.93±0.69) stages. KP1 increased grade 3 follicle index compared to KKV in primary (27.66±2.34), secondary (32.41±6.99), and tertiary (25.00±5.00) stages. Meanwhile, KP2 was in primary (26.87±6.68) and tertiary (25.00±5.00) stages. Both doses of 7.5% and 15.0% EG were able to maintain structural integrity at certain stages of preantral follicles. Secondary and tertiary follicles are the best stages in maintaining grade 3 follicular integrity with the addition of 7.5% EG.
Collaboration network of the authors
34 authors · 99 co-authorship links
Hover or tap a name to highlight its co-authors. Double-click to open the author's page.
- University of Indonesia
- Affiliation unknown
- Different organisations
- Same organisation
- Affiliation unknown
- Thicker line = more joint articles · bigger circle = more articles
Show as table
| Co-author | Organisation | Joint articles |
|---|---|---|
| M. F. Karimy | Affiliation unknown | 2 |
| H. Julendra | Affiliation unknown | 2 |
| A. Sofyan | Affiliation unknown | 2 |
| Yasman Yasman | Affiliation unknown | 2 |
| Dewi Susiloningtyas | University of Indonesia | 1 |
| Tito Latif Indra | University of Indonesia | 1 |
| Zefanya Cellina Aurawicona | University of Indonesia | 1 |
| Bintang Mahakarya Sembahen | University of Indonesia | 1 |
| Adam Heray Hutabarat | University of Indonesia | 1 |
| David Kusmawan | Affiliation unknown | 1 |
| Silvia M. Perdana | Affiliation unknown | 1 |
| Putri Irwanti S. | Affiliation unknown | 1 |
| Ira Gustina | Affiliation unknown | 1 |
| Ardi Firmansyah | Affiliation unknown | 1 |
| Moudy Soraya | Affiliation unknown | 1 |
| Justus Sievers | Affiliation unknown | 1 |
| Dionisius Denis | Affiliation unknown | 1 |
| R. Tedjo Sasmono | Affiliation unknown | 1 |
| Alfrina Hany | Affiliation unknown | 1 |
| Rustiana Tasya Ariningpraja | Affiliation unknown | 1 |
| Dewi Irawati Soeria Santoso | Affiliation unknown | 1 |
| L. Istiqomah | Affiliation unknown | 1 |
| Neng Tine Kartinah | Affiliation unknown | 1 |
| Irena Ujianti | Affiliation unknown | 1 |
| Sophie Yolanda | Affiliation unknown | 1 |
| Imelda Rosalyn Sianipar | Affiliation unknown | 1 |
| Patwa Amani | Affiliation unknown | 1 |
| Krishna Aditya Murti | Affiliation unknown | 1 |
| Dewi Irawati Soeria Santoso | Affiliation unknown | 1 |
Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.