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Expression of SARS-CoV-2 Nucleocapsid (N) Recombinant Protein Using Escherichia coli System

HAYATI Journal of Biosciences · Vol. 30 No. 3 (2023) · pp. 445–450

DOI
10.4308/hjb.30.3.445-450
ISSN
2086-4094, 1978-3019
Published
2023-01-19
Publisher
Bogor Agricultural University, Indonesia
Language
eng

Abstract

One of the main antigen that can be used for serological testing is the nucleocapsid (N) which is the most abundant viral-derived protein in SARS-CoV-2 where this virus can cause COVID19 disease. The aim of this study was to develop the SARS-CoV-2 N recombinant protein using Escherichia coli expression system. A total of 1,089 nucleotides encoding 362 amino acids of SARS-CoV-2 N was cloned to pET-14b vector. The plasmid then expressed in E. coli BL21 (DE3) and induced with 1.0 mM IPTG (Isopropyl-β-d-1-thiogalactopyranoside). The cell was harvested using denaturation lysis buffer due to inclusion body formation of SARS-CoV-2 N protein. Dialysis processed and concentrated using PEG-6000 resulted 0.992 mg/ml protein yield. Analysis of SARS-CoV-2 N recombinant protein using SDS-PAGE technique showed approximately 37.0 kDa specific band target protein. Application of this SARS-CoV-2 N recombinant protein to vaccinated and non-vaccinated antibody serum samples using ELISA technique indicated the significant result of optical density mean at 0.603 and 0.135, respectively. This study revealed that the production of SARS-COV-2 N recombinant protein could be carried out in E. coli expression system under denatured conditions, therefore the methods are more effective in producing the protein as a basic material in immuno-diagnostic assay.

Collaboration network of the authors

40 authors · 131 co-authorship links · showing the 40 most connected

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  • IPB University
  • Affiliation unknown
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Co-authorOrganisationJoint articles
DONDIN SAJUTHIAffiliation unknown5
I NENGAH BUDIARSAAffiliation unknown3
DIAH ISKANDRIATIAffiliation unknown3
Mohamad RafiAffiliation unknown2
I Wayan Teguh WibawanAffiliation unknown2
Raendi RayendraAffiliation unknown2
Fitriya Nur Annisa DewiAffiliation unknown2
Gregory K. WilkersonAffiliation unknown2
J. Mark ClineAffiliation unknown2
. PermanawatiAffiliation unknown2
Joko PamungkasAffiliation unknown2
Dyah Perwitasari-FarajallahAffiliation unknown2
TasariAffiliation unknown1
Septiana WijayantiAffiliation unknown1
L. AgusuAffiliation unknown1
SukriAffiliation unknown1
Indang DewataAffiliation unknown1
Aloysius Hari KristiantoAffiliation unknown1
Eddy SuratmanAffiliation unknown1
Akhmad YaniAffiliation unknown1
Uce LestariAffiliation unknown1
Attiya IstariniAffiliation unknown1
YuliawatiAffiliation unknown1
KamariyahAffiliation unknown1
Indah Sri Wulan SofyanAffiliation unknown1
Sofia Elisa ApriyantiAffiliation unknown1
Wildayati WildayatiAffiliation unknown1
Hera MaheshwariAffiliation unknown1
Etih SudarnikaAffiliation unknown1
Maria Fatima PalupiAffiliation unknown1
Dewi Apri AstutiIPB University1
Fitriya N. DewiAffiliation unknown1
Sela S. MariyaAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en">Expression of SARS-CoV-2 Nucleocapsid (N) Recombinant Protein Using Escherichia coli System</dc:title>
	<dc:creator xml:lang="en">Ansari, Rizki Aulia</dc:creator>
	<dc:creator xml:lang="en">Saepuloh, Uus</dc:creator>
	<dc:creator xml:lang="en">Mariya, Silmi</dc:creator>
	<dc:creator xml:lang="en">Yuliana</dc:creator>
	<dc:creator xml:lang="en">Noviana, Rachmitasari</dc:creator>
	<dc:creator xml:lang="en">Suparto, Irma Herawati</dc:creator>
	<dc:creator xml:lang="en">Darusman, Huda Shalahudin</dc:creator>
	<dc:description xml:lang="en">One of the main antigen that can be used for serological testing is the nucleocapsid (N) which is the most abundant viral-derived protein in SARS-CoV-2 where this virus can cause COVID19 disease. The aim of this study was to develop the SARS-CoV-2 N recombinant protein using Escherichia coli expression system. A total of 1,089 nucleotides encoding 362 amino acids of SARS-CoV-2 N was cloned to pET-14b vector. The plasmid then expressed in E. coli BL21 (DE3) and induced with 1.0 mM IPTG (Isopropyl-β-d-1-thiogalactopyranoside). The cell was harvested using denaturation lysis buffer due to inclusion body formation of SARS-CoV-2 N protein. Dialysis processed and concentrated using PEG-6000 resulted 0.992 mg/ml protein yield. Analysis of SARS-CoV-2 N recombinant protein using SDS-PAGE technique showed approximately 37.0 kDa specific band target protein. Application of this SARS-CoV-2 N recombinant protein to vaccinated and non-vaccinated antibody serum samples using ELISA technique indicated the significant result of optical density mean at 0.603 and 0.135, respectively. This study revealed that the production of SARS-COV-2 N recombinant protein could be carried out in E. coli expression system under denatured conditions, therefore the methods are more effective in producing the protein as a basic material in immuno-diagnostic assay.</dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2023-01-19</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://journal.ipb.ac.id/hayati/article/view/40476</dc:identifier>
	<dc:identifier>10.4308/hjb.30.3.445-450</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 30 No. 3 (2023): May 2023; 445-450</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://journal.ipb.ac.id/hayati/article/view/40476/24833</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2023 Rizki Aulia Ansari, Uus Saepuloh, Silmi Mariya, Yuliana, Rachmitasari Noviana, Irma Herawati Suparto, Huda Shalahudin Darusman</dc:rights>
</oai_dc:dc>