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Characteristics of Nitrogen-Fixing Bacteria Isolated from the Rhizosphere at Kaolin Lake, Belitung, Indonesia

HAYATI Journal of Biosciences · Vol. 32 No. 3 (2025) · pp. 611–622

DOI
10.4308/hjb.32.3.611-622
ISSN
2086-4094, 1978-3019
Published
2025-01-24
Publisher
Bogor Agricultural University, Indonesia
Language
eng

Abstract

Former kaolin mining has poor physical and chemical properties for plants. The low nutrient content makes difficult for plants to grow. Nitrogen (N2) is an important macronutrient for plants, but it can only be absorbed in the form of ammonium ions (NH4+) or nitrate ions (NO3-­). Therefore, it is necessary to transform nitrogen, which is able to change the molecular form so that it can be absorbed by plants. The strategy that can be done is using nitrogen-fixing bacteria. This study aimed to explore and analyze the diversity of nitrogen-fixing bacteria based on 16S rRNA and nifH gene from the rhizosphere of the Kaolin Lake area. The method started with bacterial isolation from rhizosphere soil samples. Selected isolates were tested for ammonium content to determine the ability of isolates to fix nitrogen. In this study, 13 isolates had the ability to fix nitrogen, two of which had a high ammonium concentration, namely RBN 5.6, and RBA 2.3. The highest ammonium concentration was produced by isolate RBN5.6 amounting to 7.55 μg/ml. Based on 16S rRNA gene identification, isolate RBN5.6 was similar to Burkholderia cepacia and isolate RBA2.3 was similar to Bacillus aquimaris. Based on the detection of the nifH gene, isolate RBN5.6 was identified as a protein-encoding dinitrogenase reductase gene group cluster from the Stutzerimonas stutzeri.

Collaboration network of the authors

40 authors · 115 co-authorship links · showing the 40 most connected

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  • IPB University
  • Affiliation unknown
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Co-authorOrganisationJoint articles
Nisa Rachmania MubarikAffiliation unknown6
Antonius SuwantoAffiliation unknown6
Anja MeryandiniAffiliation unknown5
Aris Tri WahyudiIPB University5
Sri ListiyowatiAffiliation unknown3
SRI BUDIARTIAffiliation unknown3
Sipriyadi SipriyadiAffiliation unknown2
Risky Hadi WibowoAffiliation unknown2
Maggy Thenawidjaya SuhartonoAffiliation unknown2
Hamim HamimAffiliation unknown2
Cecilia Anna SeumahuAffiliation unknown2
Dedy Duryadi SolihinAffiliation unknown2
Alina AkhdiyaAffiliation unknown2
ESTI PUSPITASARIAffiliation unknown2
Andreas Adhi SatyaAffiliation unknown2
Yepy Hardi RustamAffiliation unknown2
Apon Zaenal MustopaAffiliation unknown2
Baso ManguntungiAffiliation unknown2
Dian HendrayantiAffiliation unknown1
Dwi Andreas SantosaAffiliation unknown1
ALINA AKHADIYAAffiliation unknown1
Anik KusmiatunAffiliation unknown1
DONDIN SAJUTHIAffiliation unknown1
SUSAN SOKAAffiliation unknown1
DIAH ISKANDRIATIAffiliation unknown1
KATHARINA JESSICAAffiliation unknown1
Yulin LestariAffiliation unknown1
. MahyarudinAffiliation unknown1
RIKA RAFFIUDINAffiliation unknown1
Andri FerbiyantoAffiliation unknown1
Zulhaerati ZulhaeratiAffiliation unknown1
Yuli Siti FatmaAffiliation unknown1
Yuni Puji HastutiAffiliation unknown1
Rizal Khoirun AlfisahAffiliation unknown1
Nurfadhilla RahmadhaniAffiliation unknown1
Nabila Tsoerayya Gustia PudjasAffiliation unknown1
Lisdar I SudirmanAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en">Characteristics of Nitrogen-Fixing Bacteria Isolated from the Rhizosphere at Kaolin Lake, Belitung, Indonesia</dc:title>
	<dc:creator xml:lang="en">Nurhakiki</dc:creator>
	<dc:creator xml:lang="en">Rusmana, Iman</dc:creator>
	<dc:creator xml:lang="en">Astuti, Rika Indri</dc:creator>
	<dc:description xml:lang="en">Former kaolin mining has poor physical and chemical properties for plants. The low nutrient content makes difficult for plants to grow. Nitrogen (N2) is an important macronutrient for plants, but it can only be absorbed in the form of ammonium ions (NH4+) or nitrate ions (NO3-­). Therefore, it is necessary to transform nitrogen, which is able to change the molecular form so that it can be absorbed by plants. The strategy that can be done is using nitrogen-fixing bacteria. This study aimed to explore and analyze the diversity of nitrogen-fixing bacteria based on 16S rRNA and nifH gene from the rhizosphere of the Kaolin Lake area. The method started with bacterial isolation from rhizosphere soil samples. Selected isolates were tested for ammonium content to determine the ability of isolates to fix nitrogen. In this study, 13 isolates had the ability to fix nitrogen, two of which had a high ammonium concentration, namely RBN 5.6, and RBA 2.3. The highest ammonium concentration was produced by isolate RBN5.6 amounting to 7.55 μg/ml. Based on 16S rRNA gene identification, isolate RBN5.6 was similar to Burkholderia cepacia and isolate RBA2.3 was similar to Bacillus aquimaris. Based on the detection of the nifH gene, isolate RBN5.6 was identified as a protein-encoding dinitrogenase reductase gene group cluster from the Stutzerimonas stutzeri.</dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2025-01-24</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://journal.ipb.ac.id/hayati/article/view/59430</dc:identifier>
	<dc:identifier>10.4308/hjb.32.3.611-622</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 32 No. 3 (2025): May 2025; 611-622</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://journal.ipb.ac.id/hayati/article/view/59430/30051</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2025 Nurhakiki, Iman Rusmana, Rika Indri Astuti</dc:rights>
</oai_dc:dc>