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Properti Probiotik Isolat Bakteri Asam Laktat untuk Mengendalikan Pertumbuhan Vibrio alginolyticus pada Ikan Kerapu Macan (Epinephelus fuscoguttatus)

ILMU KELAUTAN: Indonesian Journal of Marine Sciences · Vol. 16 No. 3 (2012) · pp. 151–158

DOI
10.14710/ik.ijms.16.3.151-158
ISSN
2406-7598, 0853-7291
Published
2012-02-17
Publisher
Marine Science Department Diponegoro University
Language
id

Abstract

Tujuan penelitian ini adalah untuk menyeleksi isolat bakteri asam laktat (BAL) yang telah diisolasi dari ikan Kerapu Macan (Epinephelus fuscoguttatus) berdasarkan toleransi terhadap pH dan bile salts, patogenisitas dan kemampuannya menekan pertumbuhan Vibrio alginolyticus pada uji ko-kultur. Hasil penelitian menunjukkan bahwa dua puluh isolat BAL yang telah diuji antagonismenya terhadap V. alginolyticus pada penelitian terdahulu, dapat hidup pada pH rendah 3 dan tumbuh pada konsentrasi bile salts 0,5% kecuali isolat KSBU 13D. Semua isolat tidak patogen terhadap ikan Kerapu Macan setelah diinfeksi BAL pada konsentrasi 107 cfu/ml secara intraperitoneal (IP). Pada uji ko-kultur, dari 6 isolat BAL yang dipilih, tiga isolat (KSBU 12C, KSBU 5Da, KSBU 9) berturut-turut mampu menekan pertumbuhan V. alginolyticus, dan penambahan konsentrasi BAL terbaik adalah 107 cfu/ml. Kata kunci: bakteri asam laktat, vibriosis, patogenisitas, ko-kultur, kerapu macan The research objective was to select lactic acid bacteria which previously isolated from Tiger Grouper (Epinephelus fuscoguttatus) based on pH and bile salts tolerances, pathogenicity test and ability to decrease the growth of Vibrio alginolyticus in co-culture test. The result shows that twenty isolates which had also been examined their antagonisms against V. alginolyticus previously, were able to survive relatively low pH values 3 and able to survive at high bile salts concentrations 0.5% except KSBU 13D isolate. All isolates were nonpathogenic to Tiger Grouper after being intraperitoneally infected with the lactic acid bacteria at concentration 107 cfu/ml. In co-culture test, V. alginolyticus was reduced significantly by three isolates (KSBU 12C, KSBU 5Da, KSBU 9) from 6 isolates selected, and the best additional bacterial concentration was 107 cfu/ml. Key words: lactic acid bacteria, vibriosis, pathogenicity, kerapu macan

Collaboration network of the authors

40 authors · 100 co-authorship links · 2 across different organisations · showing the 40 most connected

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  • Universitas Gajayana Malang
  • Cenderawasih University
  • State University of Semarang
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Hartono HartonoAffiliation unknown9
Sri IswidayatiAffiliation unknown8
Muh. Ibnan SyarifAffiliation unknown6
Udi UtomoAffiliation unknown6
Tjetjep Rohendi RohidiAffiliation unknown5
Sunarto SunartoAffiliation unknown4
Wadiyo WadiyoAffiliation unknown4
Ratih Ayu PratiwinindyaAffiliation unknown3
Tjatur RebowoAffiliation unknown2
Agus CahyonoAffiliation unknown2
Tjetjep Rohendi RohidiAffiliation unknown2
Syakir SyakirAffiliation unknown2
Muh. Ibnan SyarifAffiliation unknown2
Gunadi GunadiUniversitas Gajayana Malang2
Onang MurtiyosoAffiliation unknown2
Purwanto PurwantoCenderawasih University2
Ascariena RafindaAffiliation unknown1
Rahina NugrahaniState University of Semarang1
Risca DamayantiAffiliation unknown1
Andrianus AndrianusAffiliation unknown1
Anik RahmawatiAffiliation unknown1
Fatimah AzzahrahAffiliation unknown1
Totok SumaryantoAffiliation unknown1
Arsan ShanieAffiliation unknown1
Masayu Selly SabillahAffiliation unknown1
Ahmad Fikri AriefAffiliation unknown1
Suparti SupartiAffiliation unknown1
Novysa BasriAffiliation unknown1
Suherman SuhermanAffiliation unknown1
Dhimayu Ichtiara WijayaAffiliation unknown1
Rico AndriansyahAffiliation unknown1
Ulya Zakaria AzmiAffiliation unknown1
Fitriani FitrianiAffiliation unknown1
Tria Ayu DiniAffiliation unknown1
Arrini Shabrina AnshorAffiliation unknown1
Aziz Ma'sumAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) http://ejournal.undip.ac.id/index.php/ijms/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en-US">Properti Probiotik Isolat Bakteri Asam Laktat untuk Mengendalikan Pertumbuhan Vibrio alginolyticus pada Ikan Kerapu Macan (Epinephelus fuscoguttatus)</dc:title>
	<dc:creator>Nursyirwani, Nursyirwani</dc:creator>
	<dc:creator>Asmara, W.</dc:creator>
	<dc:creator>Wahyuni, A.E.T.H.</dc:creator>
	<dc:creator>Triyanto, Triyanto</dc:creator>
	<dc:description xml:lang="en-US">  Tujuan penelitian ini adalah untuk menyeleksi isolat bakteri asam laktat (BAL) yang telah diisolasi dari ikan Kerapu Macan (Epinephelus fuscoguttatus) berdasarkan toleransi terhadap pH dan bile salts, patogenisitas dan kemampuannya menekan pertumbuhan Vibrio alginolyticus pada uji ko-kultur. Hasil penelitian menunjukkan bahwa dua puluh isolat BAL yang telah diuji antagonismenya terhadap V. alginolyticus pada penelitian terdahulu, dapat hidup pada pH rendah 3 dan tumbuh pada konsentrasi bile salts 0,5% kecuali isolat KSBU 13D.  Semua isolat tidak patogen terhadap ikan Kerapu Macan setelah diinfeksi BAL pada konsentrasi 107 cfu/ml secara intraperitoneal (IP). Pada uji ko-kultur, dari 6 isolat BAL yang dipilih, tiga isolat (KSBU 12C, KSBU 5Da, KSBU 9) berturut-turut mampu menekan pertumbuhan V. alginolyticus, dan penambahan konsentrasi BAL terbaik adalah 107 cfu/ml. Kata kunci: bakteri asam laktat, vibriosis,  patogenisitas, ko-kultur, kerapu macan The research objective was to select lactic acid bacteria which previously isolated from Tiger Grouper (Epinephelus fuscoguttatus) based on pH and bile salts tolerances, pathogenicity test and ability to decrease the growth of Vibrio alginolyticus in co-culture test. The result shows that twenty isolates which had also been examined their antagonisms against V. alginolyticus previously, were able to survive relatively low pH  values 3 and able to survive at high bile salts concentrations 0.5% except KSBU 13D isolate.  All isolates were nonpathogenic to Tiger Grouper after being intraperitoneally infected with the lactic acid bacteria at concentration 107 cfu/ml. In co-culture test, V. alginolyticus was reduced significantly by three isolates (KSBU 12C, KSBU 5Da, KSBU 9) from 6 isolates selected, and the best additional bacterial concentration was  107 cfu/ml. Key words: lactic acid bacteria,  vibriosis,  pathogenicity,  kerapu macan</dc:description>
	<dc:publisher xml:lang="en-US">Marine Science Department Diponegoro University</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2012-02-17</dc:date>
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	<dc:identifier>https://ejournal.undip.ac.id/index.php/ijms/article/view/1958</dc:identifier>
	<dc:identifier>10.14710/ik.ijms.16.3.151-158</dc:identifier>
	<dc:source xml:lang="en-US">ILMU KELAUTAN: Indonesian Journal of Marine Sciences; Vol 16, No 3 (2011): Ilmu Kelautan; 151-158</dc:source>
	<dc:source>2406-7598</dc:source>
	<dc:source>0853-7291</dc:source>
	<dc:language>id</dc:language>
	<dc:relation>https://ejournal.undip.ac.id/index.php/ijms/article/view/1958/1718</dc:relation>
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