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The Quality of Indo-Pacific Bottlenose Dolphin (Tursiops aduncus) Sperm Following Liquid-storage in Low Temperature

ILMU KELAUTAN: Indonesian Journal of Marine Sciences · Vol. 29 No. 4 (2024) · pp. 456–466

DOI
10.14710/ik.ijms.29.4.456-466
ISSN
2406-7598, 0853-7291
Published
2024-12-11
Publisher
Marine Science Department Diponegoro University
Language
eng
License
creativecommons.org/licenses/by-sa/4.0

Abstract

Indo-Pacific bottlenose dolphin (Tursiops aduncus) is a marine mammal that lives in relatively small populations. The geographic ranges of this species are susceptible to the effects of human activities, thereby necessitating conservation efforts to prevent extinction. Therefore, this study aimed to evaluate the daily quality of dolphin sperm after several days of refrigeration. The sperm of two male dolphins were stored at 4oC for 4 days, and the quality was observed daily to determine the motility, viability, membrane integrity, and sperm abnormalities. Sperm samples were divided into four groups, consisting of two centrifuged followed by the removal of seminal plasma, and two groups without centrifugation, containing 100x106 and 200x106 sperm/ml each. After liquid storage, the motility of sperm was 63-75% with no significant reduction in the first 3 days. Sperm viability following storage was 65-75% and the percentage with abnormal morphology ranged from 2-6%. Furthermore, there was no significant increase in abnormal morphology of sperm on any day of storage for 3 days. Sperm membrane integrity was 36-49%, with no significant reduction in the membrane integrity in the first 2 days. There was no significant difference in sperm quality, although centrifugation and removal of seminal plasm had a slight effect. The results of this study showed that Indo-Pacific bottlenose dolphin sperm could be stored for a short period as liquid storage while maintaining a quality that allows for future use.

Keywords

Collaboration network of the authors

40 authors · 146 co-authorship links · showing the 40 most connected

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  • IPB University
  • Affiliation unknown
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Show as table
Co-authorOrganisationJoint articles
Arief BoedionoAffiliation unknown5
Kusdiantoro MohamadAffiliation unknown5
KAZUHIRO KIKUCHIAffiliation unknown4
BAMBANG PURWANTARAAffiliation unknown4
Elsi RahmadhaniAffiliation unknown3
Ronald TariganAffiliation unknown3
Yanti SulistyanaAffiliation unknown3
Mohamad Agus SetiadiAffiliation unknown3
Sri Anna MarliyatiAffiliation unknown2
Vista BudiariatiAffiliation unknown2
Dwi BudionoAffiliation unknown2
Syahruddin SaidAffiliation unknown2
Lilis KhotijahAffiliation unknown2
Aeni NurlatifahAffiliation unknown2
Aisyah Fidela SiregarIPB University2
Mokhamad FahrudinIPB University2
Rahayu Dewi SoeyonoAffiliation unknown1
Rita IsmawatiAffiliation unknown1
Hardinsyah HardinsyahAffiliation unknown1
Choirul Anna Nur AfifahAffiliation unknown1
Yuni NurwatiAffiliation unknown1
Nabilla Ersya AudinaAffiliation unknown1
Adi PranotoAffiliation unknown1
Aryani Sismin SatyaningtijasAffiliation unknown1
Damiana Rita EkastutiAffiliation unknown1
I Ketut Mudite AdnyaneAffiliation unknown1
TUTY LASWARDI YUSUFAffiliation unknown1
Berry JuliandiAffiliation unknown1
Ratih RinendyaputriAffiliation unknown1
Feni Dwi Kartika GuloAffiliation unknown1
Noer Muhammad Dliyaul HaqAffiliation unknown1
Diah PristihadiAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) http://ejournal.undip.ac.id/index.php/ijms/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en-US">The Quality of Indo-Pacific Bottlenose Dolphin (Tursiops aduncus) Sperm Following Liquid-storage in Low Temperature</dc:title>
	<dc:creator>Fahrudin, Mokhamad</dc:creator>
	<dc:creator>Gusdinar, Rizal</dc:creator>
	<dc:creator>Arifiantini, Raden Iis</dc:creator>
	<dc:creator>Prasetyaningtyas, Wahono Esthi</dc:creator>
	<dc:creator>Adnyane, I Ketut Mudite</dc:creator>
	<dc:creator>Elmanaviean, Muhammad</dc:creator>
	<dc:creator>Nugraha, Arifin Budiman</dc:creator>
	<dc:creator>Karja, Ni Wayan Kurniani</dc:creator>
	<dc:subject xml:lang="en-US">dolphin; sperm;  preservation; motility; viability; liquid-storage</dc:subject>
	<dc:description xml:lang="en-US">Indo-Pacific bottlenose dolphin (Tursiops aduncus) is a marine mammal that lives in relatively small populations. The geographic ranges of this species are susceptible to the effects of human activities, thereby necessitating conservation efforts to prevent extinction. Therefore, this study aimed to evaluate the daily quality of dolphin sperm after several days of refrigeration. The sperm of two male dolphins were stored at 4oC for 4 days, and the quality was observed daily to determine the motility, viability, membrane integrity, and sperm abnormalities. Sperm samples were divided into four groups, consisting of two centrifuged followed by the removal of seminal plasma, and two groups without centrifugation, containing 100x106 and 200x106 sperm/ml each. After liquid storage, the motility of sperm was 63-75% with no significant reduction in the first 3 days. Sperm viability following storage was 65-75% and the percentage with abnormal morphology ranged from 2-6%. Furthermore, there was no significant increase in abnormal morphology of sperm on any day of storage for 3 days. Sperm membrane integrity was 36-49%, with no significant reduction in the membrane integrity in the first 2 days. There was no significant difference in sperm quality, although centrifugation and removal of seminal plasm had a slight effect. The results of this study showed that Indo-Pacific bottlenose dolphin sperm could be stored for a short period as liquid storage while maintaining a quality that allows for future use. </dc:description>
	<dc:publisher xml:lang="en-US">Marine Science Department Diponegoro University</dc:publisher>
	<dc:date>2024-12-11</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US"></dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://ejournal.undip.ac.id/index.php/ijms/article/view/65906</dc:identifier>
	<dc:identifier>10.14710/ik.ijms.29.4.456-466</dc:identifier>
	<dc:source xml:lang="en-US">ILMU KELAUTAN: Indonesian Journal of Marine Sciences; Vol 29, No 4 (2024): Ilmu Kelautan; 456-466</dc:source>
	<dc:source>2406-7598</dc:source>
	<dc:source>0853-7291</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://ejournal.undip.ac.id/index.php/ijms/article/view/65906/pdf</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2024 ILMU KELAUTAN: Indonesian Journal of Marine Sciences</dc:rights>
	<dc:rights xml:lang="en-US">https://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>