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Purity Testing of Cultivar Melon DNA Level (Cucumis melo L.) “Gama Melon Basket” using Surface Plasmon Resonance (SPR) Based on Silver Nanoparticle

INDONESIAN JOURNAL OF APPLIED PHYSICS · Vol. 5 No. 01 (2015) · pp. 16–22

DOI
10.13057/ijap.v5i01.254
ISSN
2477-6416, 2089-0133
Published
2015-12-16
Publisher
Department of Physics, Sebelas Maret University
Language
eng

Abstract

DNA level of purity has been tested by observing the phenomenon of surface plasmon resonance (SPR) in silver film + silver (Ag) nanoparticles system as DNA sensor using Kretschmann configuration. Surface plasmon resonance (SPR) phenomenon observed in the resonance condition of reflectance spectrum vary with incident angle of laser, beamed toward prism. System is built as a model for DNA purity test instrument that give higher accuracy. DNA purity testing was conducted by placing DNA layer with various ratio on prism in order to get the angle shift value in each sample as a result. Purity level of DNA indicates the DNA quality based on the ratio between the amount of pure DNA compared to the amount of impurity materials (protein). After the deposition of DNA in ratio 1,774, 1,838 and 1,916 SPR angle shifted, and respectively. Characteristic differences of DNA with different purity (ratio) are shown by reflectance value that is getting higher at a smaller ratio, indicates that in the higher DNA ratio the angle shift is getting smaller. SPR angle shift which is occurred before and after DNA deposition shows the sensitivity of SPR as DNA sensor.

Collaboration network of the authors

40 authors · 131 co-authorship links · 2 across different organisations · showing the 40 most connected

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • Muria Kudus University
  • Udayana University
  • Satya Wacana Christian University
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Akmal RizaAffiliation unknown3
Syafiul MuzidMuria Kudus University1
Muhammad Taufiq HidayatMuria Kudus University1
Khadijah Binti Mohd Khambali @ HambaliAffiliation unknown1
Muhammad FauziUdayana University1
Lesis AndreAffiliation unknown1
Josanti NurhidayahAffiliation unknown1
Syahrul GafurAffiliation unknown1
Atina HusnaAffiliation unknown1
Rita MeriAffiliation unknown1
DaniahAffiliation unknown1
Edo PutraAffiliation unknown1
Meta Yupitri ZoningsihAffiliation unknown1
Aqilla TunnaziaAffiliation unknown1
Endang SukinahAffiliation unknown1
Debi Kumala PutraAffiliation unknown1
Saudatul FitriAffiliation unknown1
Evangs MailoaSatya Wacana Christian University1
Azharsyah IbrahimAffiliation unknown1
Isnaliana IsnalianaAffiliation unknown1
Ayumiati AyumiatiAffiliation unknown1
Vira Clarissa KsrisyaAffiliation unknown1
Muksal MuksalAffiliation unknown1
Muizatul AspiraAffiliation unknown1
Lailatussaadah LailatussaadahAffiliation unknown1
Sri MutiaAffiliation unknown1
Salma HayatiAffiliation unknown1
Cut Intan HayatiAffiliation unknown1
Cut Lisa Nun ZikirAffiliation unknown1
Rachmi MeutiaAffiliation unknown1
Ghulaman ZakiiyaAffiliation unknown1
Jalilah JalilahAffiliation unknown1
Nazar NazarAffiliation unknown1
ABDUL MANANAffiliation unknown1
Nurmalia NurmaliaAffiliation unknown1
Zaki Saptari SaldiAffiliation unknown1
Purba PurnamaAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://jurnal.uns.ac.id/ijap/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en-US">Purity Testing of Cultivar Melon DNA Level (Cucumis melo L.) “Gama Melon Basket” using Surface Plasmon Resonance (SPR) Based on Silver Nanoparticle</dc:title>
	<dc:creator>T, Thoyibi</dc:creator>
	<dc:creator>Arifin, Muhammad</dc:creator>
	<dc:creator>Abraha, Kamsul</dc:creator>
	<dc:description xml:lang="en-US">DNA level of purity has been tested by observing the phenomenon of surface plasmon resonance (SPR) in silver film + silver (Ag) nanoparticles system as DNA sensor using Kretschmann configuration. Surface plasmon resonance (SPR) phenomenon observed in the resonance condition of reflectance spectrum vary with incident angle of laser, beamed toward prism. System is built as a model for DNA purity test instrument that give higher accuracy. DNA purity testing was conducted by placing DNA layer with various ratio on prism in order to get the angle shift value in each sample as a result. Purity level of DNA indicates the DNA quality based on the ratio between the amount of pure DNA compared to the amount of impurity materials (protein). After the deposition of DNA in ratio 1,774, 1,838 and 1,916 SPR angle shifted ,  and  respectively. Characteristic differences of DNA with different purity (ratio) are shown by reflectance value that is getting higher at a smaller ratio, indicates that in the higher DNA ratio the angle shift is getting smaller. SPR angle shift which is occurred before and after DNA deposition shows the sensitivity of SPR as DNA sensor.</dc:description>
	<dc:publisher xml:lang="en-US">Department of Physics, Sebelas Maret University</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2015-12-16</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://jurnal.uns.ac.id/ijap/article/view/254</dc:identifier>
	<dc:identifier>10.13057/ijap.v5i01.254</dc:identifier>
	<dc:source xml:lang="en-US">INDONESIAN JOURNAL OF APPLIED PHYSICS; Vol 5, No 01 (2015): April; 16-22</dc:source>
	<dc:source>2477-6416</dc:source>
	<dc:source>2089-0133</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://jurnal.uns.ac.id/ijap/article/view/254/230</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2015 INDONESIAN JOURNAL OF APPLIED PHYSICS</dc:rights>
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