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T47D cells arrested at G2M and Hyperploidy Formation Induced by a Curcumin’s Analogue PGV-1

Indonesian Journal of Biotechnology · Vol. 12 No. 2 (2007)

DOI
10.22146/ijbiotech.7776
ISSN
2089-2241, 0853-8654
Published
2007-12-09
Publisher
Universitas Gadjah Mada
Language
eng

Abstract

its chemical structure than curcumin. As a curcumin analogue, PGV-1 was considered to have anticanceractivities. This research was conducted to study the effect of PGV-1 on the cycle progression of T47D cells. Cytotoxiceffects of PGV-1 on T47D cells were determined using MTT assay, and the the effect on cell cycle progressionwas carried out using flowcytometry. Western blot analysis was used to analyze protein expression correspondingto cell cycle progression. The result showed that at the concentration of 2.5 μM PGV-1 inhibited cell cycleprogression through G2/M arrest and induced of cells hyperploidy formation. The hyperploidy formation inducedby PGV-1 was related to the increase of cdc-2 expression. PGV-1 2.5 μM elevated the level of p21 CIP/KIPthrough p53- independent manner. Apoptosis was also induced by PGV-1 at early phase of treatment indicated byPARP cleavage due to activation of caspase-3/7 after 12 h treatment. The results above suggest that PGV-1 inhibitsthe growth of T47D cells targeted on microtubules.Keywords: PGV-1, G2/M arrest, apoptosis, p21

Collaboration network of the authors

40 authors · 122 co-authorship links · showing the 40 most connected

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  • University of Brawijaya
  • IPB University
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Ratna Asmah SusidartiAffiliation unknown17
Adam HermawanAffiliation unknown17
Riris Istighfari JenieAffiliation unknown14
Muthi’ IkawatiAffiliation unknown11
Rohmad Yudi UtomoAffiliation unknown8
Dyaningtyas Dewi Pamungkas PutriAffiliation unknown7
Sari HaryantiAffiliation unknown6
Dhania NovitasariAffiliation unknown4
Retno MurwantiAffiliation unknown4
Faaza Aulia RahmanAffiliation unknown4
Desty Restia RahmawatiAffiliation unknown4
Sri HandayaniUniversity of Brawijaya3
Ummi Maryam ZulfinAffiliation unknown3
Anindyajati AnindyajatiAffiliation unknown3
Larasati LarasatiIPB University3
Erlina RivantiAffiliation unknown3
Nunuk Aries NurulitaAffiliation unknown3
M. MurwantokoAffiliation unknown2
Sendy JunediAffiliation unknown2
LANGKAH SEMBIRINGAffiliation unknown2
Suwijiyo PramonoAffiliation unknown2
Zalinar UdinAffiliation unknown2
Yogi ErtantoAffiliation unknown2
Lailatul QodriaAffiliation unknown2
Gergorius Gena MaranAffiliation unknown2
Aulia Nur SeptianiAffiliation unknown2
Andi SuhendiAffiliation unknown2
Sofa FaridaAffiliation unknown2
Beni LestariAffiliation unknown2
Asri Mega PutriAffiliation unknown2
Hilyatul FadliyahAffiliation unknown2
Indri KusharyantiAffiliation unknown2
Yonika Arum LarasatiAffiliation unknown2
Retno SunarminingsihAffiliation unknown2
Sofia MubarikaAffiliation unknown2

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">T47D cells arrested at G2M and Hyperploidy Formation Induced by a Curcumin’s Analogue PGV-1</dc:title>
	<dc:creator>Da’i, Muhammad</dc:creator>
	<dc:creator>Jenie, Umar Anggara</dc:creator>
	<dc:creator>AM, Supardjan</dc:creator>
	<dc:creator>Kawaichi, Masashi</dc:creator>
	<dc:creator>Meiyanto, Edy</dc:creator>
	<dc:description xml:lang="en-US">its chemical structure than curcumin. As a curcumin analogue, PGV-1 was considered to have anticanceractivities. This research was conducted to study the effect of PGV-1 on the cycle progression of T47D cells. Cytotoxiceffects of PGV-1 on T47D cells were determined using MTT assay, and the the effect on cell cycle progressionwas carried out using flowcytometry. Western blot analysis was used to analyze protein expression correspondingto cell cycle progression. The result showed that at the concentration of 2.5 μM PGV-1 inhibited cell cycleprogression through G2/M arrest and induced of cells hyperploidy formation. The hyperploidy formation inducedby PGV-1 was related to the increase of cdc-2 expression. PGV-1 2.5 μM elevated the level of p21 CIP/KIPthrough p53- independent manner. Apoptosis was also induced by PGV-1 at early phase of treatment indicated byPARP cleavage due to activation of caspase-3/7 after 12 h treatment. The results above suggest that PGV-1 inhibitsthe growth of T47D cells targeted on microtubules.Keywords: PGV-1, G2/M arrest, apoptosis, p21</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:date>2007-12-09</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/7776</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.7776</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 12, No 2 (2007)</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/7776/6026</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2015 Indonesian Journal of Biotechnology</dc:rights>
</oai_dc:dc>