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Detection and Cloning of a Gene Involved in Zwitermicin A Synthesis from Plant Growth Promoting Rhizobacteria of Bacillus sp CR64

Indonesian Journal of Biotechnology · Vol. 15 No. 1 (2010)

DOI
10.22146/ijbiotech.7818
ISSN
2089-2241, 0853-8654
Published
2010-06-02
Publisher
Universitas Gadjah Mada
Language
eng

Abstract

Utilization of soil bacteria as biocontrol agent is becoming popular due to its valuable and effective mechanisms to suppress plant pathogenic microbes. We have previously isolated Bacillus sp, designated as Bacillus sp CR64, which exhibited effective plant growth promoting and antifungal activities. In this study, CR64 was examined in inhibiting the growth of Rhizoctonia solani, the causing agent of root rot disease. Partial sequence analysis of 16S rRNA gene revealed that this isolate similar with Bacillus cereus (94%). Furthermore, a gene designated zmaR was detected by means of specific amplification of DNA fragment approximately 950 bp. This fragment was then cloned onto pCRII-TOPO (3.9 kb) and sequenced using DNA sequencer ABI PRISM 310. Sequence analysis revealed that it had highest homology with the ZmaR protein (89% identity; 90% similarity) of B. thuringiensis serovar kurstaki (AAF82729.2). Alignment analysis with other ZmaR sequences from other antibiotic-producing Bacilli exhibited an almost fully conserved region within ZmaR sequences.Key words: PGPR, Bacillus sp CR64, Zwitermicin A, Cloning, Antifungal.

Collaboration network of the authors

40 authors · 117 co-authorship links · 5 across different organisations · showing the 40 most connected

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • IPB University
  • Institut Teknologi Sepuluh Nopember
  • Khairun University
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Antonius SuwantoAffiliation unknown9
Iman RusmanaAffiliation unknown7
Anja MeryandiniAffiliation unknown7
SRI BUDIARTIAffiliation unknown5
ESTI PUSPITASARIAffiliation unknown5
Andreas Adhi SatyaAffiliation unknown5
Yepy Hardi RustamAffiliation unknown4
Munti YuhanaIPB University3
Sri ListiyowatiAffiliation unknown3
Sipriyadi SipriyadiAffiliation unknown2
Risky Hadi WibowoAffiliation unknown2
Maggy Thenawidjaya SuhartonoAffiliation unknown2
Suryo WiyonoAffiliation unknown2
Yulin LestariAffiliation unknown2
Abdul MunifInstitut Teknologi Sepuluh Nopember2
Abdjad Asih NawangsihAffiliation unknown2
Widanarni WidanarniIPB University2
BRAMANTYO JATI PRASOJOAffiliation unknown2
. QATRUNNADAAffiliation unknown2
Alina AkhdiyaAffiliation unknown2
Waode MunaeniKhairun University1
Mia SetiawatiIPB University1
Iah Novi MaslahahAffiliation unknown1
Tri Asmira DamayantiAffiliation unknown1
HENDRA PARDEDEAffiliation unknown1
Triadiati TriadiatiAffiliation unknown1
ANGELIA REZTY FITRIANI SITUMORANGAffiliation unknown1
Ari SusilowatiAffiliation unknown1
LATIFAH KOSIM DARUSMANAffiliation unknown1
Marini WijayantiAffiliation unknown1
Martin EngelhauptAffiliation unknown1
Zulhaerati ZulhaeratiAffiliation unknown1
Hamim HamimAffiliation unknown1
Yuli Siti FatmaAffiliation unknown1
Nurfadhilla RahmadhaniAffiliation unknown1
Muhammad Faiz AmriAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en-US">Detection and Cloning of a Gene Involved in Zwitermicin A Synthesis from Plant Growth Promoting Rhizobacteria of Bacillus sp CR64</dc:title>
	<dc:creator>Wahyudi, Aris Tri</dc:creator>
	<dc:creator>Astuti, Rika Indri</dc:creator>
	<dc:creator>Mubarik, Nisa Rachmania</dc:creator>
	<dc:creator>Faulina, Sarah Asih</dc:creator>
	<dc:subject xml:lang="en-US"></dc:subject>
	<dc:description xml:lang="en-US">Utilization of soil bacteria as biocontrol agent is becoming popular due to its valuable and effective mechanisms to suppress plant pathogenic microbes. We have previously isolated Bacillus sp, designated as Bacillus sp CR64, which exhibited effective plant growth promoting and antifungal activities. In this study, CR64 was examined in inhibiting the growth of Rhizoctonia solani, the causing agent of root rot disease. Partial sequence analysis of 16S rRNA gene revealed that this isolate similar with Bacillus cereus (94%). Furthermore, a gene designated zmaR was detected by means of specific amplification of DNA fragment approximately 950 bp. This fragment was then cloned onto pCRII-TOPO (3.9 kb) and sequenced using DNA sequencer ABI PRISM 310. Sequence analysis revealed that it had highest homology with the ZmaR protein (89% identity; 90% similarity) of B. thuringiensis serovar kurstaki (AAF82729.2). Alignment analysis with other ZmaR sequences from other antibiotic-producing Bacilli exhibited an almost fully conserved region within ZmaR sequences.Key words : PGPR, Bacillus sp CR64, Zwitermicin A, Cloning, Antifungal.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2010-06-02</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/7818</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.7818</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 15, No 1 (2010)</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/7818/6059</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2015 Indonesian Journal of Biotechnology</dc:rights>
</oai_dc:dc>