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Ethanol Fermentation on Mixed Sugars Using Mixed Culture of Two Yeast Strains

Indonesian Journal of Biotechnology · Vol. 18 No. 2 (2013) · pp. 116–122

DOI
10.22146/ijbiotech.7880
ISSN
2089-2241, 0853-8654
Published
2013-12-03
Publisher
Universitas Gadjah Mada
Language
eng

Abstract

The objective of this study was to evaluate the use of mixed cultures of the recommended yeast strainsfrom a previous study on ethanol fermentation using a substrate mixture consisting of sucrose, glucose, andfructose. There were three mixed (combination) cultures namely OUT7096/OUT7913, OUT7096/OUT7921,and OUT7913/OUT7921. The fermentation medium contained sugar mixture consisting of glucose, fructose,and sucrose with a composition generally close to the composition of sugars in sweet sorghum juice. Overall,fermentation is carried out in two stages. First fermentation was performed using the three mixed culturesto determine the best combination based on the concentration of ethanol produced and the concentration ofresidual sugar. Second fermentation was conducted using the best mixed culture obtained from the fi rst stage.This second stage was intended to describe the pattern of the changes in the concentration of ethanol, sugarsand biomass during the fermentation progresses and to determine some kinetic parameters namely ethanolyield (Yp/s), growth yield (Yx/s) and specifi c growth rate (μ). The results of the fi rst fermentation showed thatthe best mixed culture was OUT7913/OUT7921 and the subsequent fermentation using this culture providethe highest ethanol yield (Yp/s) = 0.47 g.g-1 that was reached at 53rd hour, growth yield (Yx/s) = 0.425 g.g-1, andμ = 0.12 hour-1.Keywords: fermentation, ethanol, mixed culture, mixed sugar

Keywords

Collaboration network of the authors

40 authors · 95 co-authorship links · showing the 40 most connected

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  • Universitas Gadjah Mada
  • IPB University
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Jaka WidadaUniversitas Gadjah Mada5
Pudji HastutiAffiliation unknown3
Siti KabirunAffiliation unknown3
Sri WedhastriAffiliation unknown3
Indro PrastowoAffiliation unknown2
Yekti Asih PurwestriAffiliation unknown2
Triwibowo YuwonoAffiliation unknown2
Akbar ArofatullahAffiliation unknown2
Rusdi EvizalAffiliation unknown2
Dinar Mindrati FardhaniAffiliation unknown2
Muhammad Saifur RohmanAffiliation unknown2
Teguh AriyantoAffiliation unknown1
Rini UmiyatiAffiliation unknown1
Ria MillatiAffiliation unknown1
SUPRIYANTO SUPRIYANTOAffiliation unknown1
Inasanti Pandan WangiAffiliation unknown1
Hary SulistyoAffiliation unknown1
Triyanto TriyantoAffiliation unknown1
Siti Lusi Arum SariAffiliation unknown1
Zuprizal ZuprizalAffiliation unknown1
Yukio MukaiAffiliation unknown1
Kenji IrieAffiliation unknown1
Hiroyuki ArakiAffiliation unknown1
Yasuji OshimaAffiliation unknown1
Lucia Dhiantika WitasariAffiliation unknown1
Dionysius Andang Arif WibawaAffiliation unknown1
Pramudji HastutiAffiliation unknown1
Pintaka KusumaningtyasAffiliation unknown1
Dhani SuryawanAffiliation unknown1
Yuliana PrahastiwiAffiliation unknown1
Yohanna Anisa IndriyaniIPB University1
Heri HendrosatriyoAffiliation unknown1
Sari DarmasiwiAffiliation unknown1
Muhammad Nur CahyantoAffiliation unknown1
Maulina NurikasariAffiliation unknown1
Charlie Ester de FretesAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Ethanol Fermentation on Mixed Sugars Using Mixed Culture of Two Yeast Strains</dc:title>
	<dc:creator>Jasman, J.</dc:creator>
	<dc:creator>Prijambada, Irfan Dwidya</dc:creator>
	<dc:creator>Hidayat, Chusnul</dc:creator>
	<dc:creator>Widianto, Donny</dc:creator>
	<dc:subject xml:lang="en-US">fermentation; ethanol; mixed culture; mixed sugar</dc:subject>
	<dc:description xml:lang="en-US">The objective of this study was to evaluate the use of mixed cultures of the recommended yeast strainsfrom a previous study on ethanol fermentation using a substrate mixture consisting of sucrose, glucose, andfructose. There were three mixed (combination) cultures namely OUT7096/OUT7913, OUT7096/OUT7921,and OUT7913/OUT7921. The fermentation medium contained sugar mixture consisting of glucose, fructose,and sucrose with a composition generally close to the composition of sugars in sweet sorghum juice. Overall,fermentation is carried out in two stages. First fermentation was performed using the three mixed culturesto determine the best combination based on the concentration of ethanol produced and the concentration ofresidual sugar. Second fermentation was conducted using the best mixed culture obtained from the fi rst stage.This second stage was intended to describe the pattern of the changes in the concentration of ethanol, sugarsand biomass during the fermentation progresses and to determine some kinetic parameters namely ethanolyield (Yp/s), growth yield (Yx/s) and specifi c growth rate (μ). The results of the fi rst fermentation showed thatthe best mixed culture was OUT7913/OUT7921 and the subsequent fermentation using this culture providethe highest ethanol yield (Yp/s) = 0.47 g.g-1 that was reached at 53rd hour, growth yield (Yx/s) = 0.425 g.g-1, andμ = 0.12 hour-1.Keywords : fermentation, ethanol, mixed culture, mixed sugar</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2013-12-03</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/7880</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.7880</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 18, No 2 (2013); 116-122</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/7880/6121</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2015 Indonesian Journal of Biotechnology</dc:rights>
</oai_dc:dc>