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Detection and identifcation of adherence genes of intestinal-origin Lactobacillus and Pediococcus strains grown on gastric mucin in vitro

Indonesian Journal of Biotechnology · Vol. 20 No. 2 (2015) · pp. 174–181

DOI
10.22146/ijbiotech.24346
ISSN
2089-2241, 0853-8654
Published
2015-12-01
Publisher
Universitas Gadjah Mada
Language
eng
License
creativecommons.org/licenses/by-sa/4.0

Abstract

One of the primary selection criteria for potential probiotics is the ability to adhere to the host gastrointestinal tract. This study evaluated the in vitro adhesion ability on gastric mucin of two Lactobacillus casei strains (AP and AG) and two Pediococcus acidilactici strains (BE and BK), and identifed the corresponding genes responsible for adherence. Adhesion assays were performed in 96-well polystyrene microtiter plates using gastric mucin from porcine stomach as the matrix. An in vitro study on gastric mucin revealed that lactobacilli had a greater adherence ability compared with pediococci strains. The potential adherence genes were investigated using polymerase chain reaction (PCR) technology. Using specifc primers, PCR studies amplifed 150 base pairs of a potential mub gene and 161 base pairs of a potential ef-Tu gene, but no amplifed bands for potential map and bac genes were obtained. Sequence comparisons showed that the 150 and 161 amplifed base pairs were respectively homologous to the mub of Pediococcus acidilactici and ef-Tu genes of Lactobacillus paracasei. We concluded that the adherence ability of two strains of Lactobacillus casei (AP and AG) and two strains of Pediococcus acidilactici (BE and BK) on gastric mucin is in accordance with the presence of ef-Tu and mub genes. High level attachment in lactobacilli is likely to correlate with the ef-Tu gene, which is a lactobacilli-specifc adhesive gene.

Keywords

Collaboration network of the authors

40 authors · 81 co-authorship links · 30 across different organisations · showing the 40 most connected

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • Universitas Negeri Surabaya
  • Universitas Gadjah Mada
  • National Research and Innovation Agency
  • Universitas Respati Yogyakarta
  • Universitas Widyatama
  • Sriwijaya University
  • Edith Cowan University
  • Other organisations
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Michael Haryadi WibowoAffiliation unknown4
Wayan T. ArtamaAffiliation unknown3
Tiyas Tono TaufiqAffiliation unknown3
Budi JatmikoUniversitas Negeri Surabaya2
Arum SetiawanSriwijaya University2
Mammed SagiUniversitas Gadjah Mada2
Istriyati IstriyatiUniversitas Gadjah Mada2
Aprila NiravitaAffiliation unknown2
Muhammad Reza MaulanaAffiliation unknown2
Fauziah RamadhaniAffiliation unknown2
Mukhamad NurhadiAffiliation unknown2
Hadi NurAffiliation unknown2
Sin Yuan LaiAffiliation unknown2
Marsetyawan HNE SoesatyoAffiliation unknown2
Tiarma Talenta TheresiaAffiliation unknown2
Jaka WidadaUniversitas Gadjah Mada2
M. MustofaAffiliation unknown2
A. AlimuddinAffiliation unknown2
SEBASTIAN MARGINOAffiliation unknown2
Sri DarmawatiAffiliation unknown2
LANGKAH SEMBIRINGAffiliation unknown2
Tri UntariAffiliation unknown2
Bambang Tejo PremonoAffiliation unknown2
MurnawanUniversitas Widyatama1
Rosalin SamihardjoAffiliation unknown1
Rini WidyaningrumUniversitas Gadjah Mada1
Ferry JieEdith Cowan University1
Deni HadianaNational Research and Innovation Agency1
Zulmi RamdaniSunan Gunung Djati State Islamic University Bandung1
Andi AmriUniversitas Muhammadiyah Prof Dr Hamka1
Jaka WarsihnaIndonesia Open University1
Darwis ChandraIndonesia University of Education1
E. SopandiNational Research and Innovation Agency1
Martini MartiniUniversitas Negeri Surabaya1
Muhamad Arif MahdiannurUniversitas Negeri Surabaya1
Ahmad QosyimUniversitas Negeri Surabaya1
Elok SudibyoUniversitas Negeri Surabaya1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en-US">Detection and identifcation of adherence genes of intestinal-origin Lactobacillus and Pediococcus strains grown on gastric mucin in vitro</dc:title>
	<dc:creator>Widodo, W.</dc:creator>
	<dc:creator>Lestari, Sri</dc:creator>
	<dc:creator>Asmara, Widya</dc:creator>
	<dc:subject xml:lang="en-US">Adherence genes; intestinal origin; Lactic Acid Bacteria</dc:subject>
	<dc:description xml:lang="en-US">One of the primary selection criteria for potential probiotics is the ability to adhere to the host gastrointestinal tract. This study evaluated the in vitro adhesion ability on gastric mucin of two Lactobacillus casei strains (AP and AG) and two Pediococcus acidilactici strains (BE and BK), and identifed the corresponding genes responsible for adherence. Adhesion assays were performed in 96-well polystyrene microtiter plates using gastric mucin from porcine stomach as the matrix. An in vitro study on gastric mucin revealed that lactobacilli had a greater adherence ability compared with pediococci strains. The potential adherence genes were investigated using polymerase chain reaction (PCR) technology. Using specifc primers, PCR studies amplifed 150 base pairs of a potential mub gene and 161 base pairs of a potential ef-Tu gene, but no amplifed bands for potential map and bac genes were obtained. Sequence comparisons showed that the 150 and 161 amplifed base pairs were respectively homologous to the mub of Pediococcus acidilactici and ef-Tu genes of Lactobacillus paracasei. We concluded that the adherence ability of two strains of Lactobacillus casei (AP and AG) and two strains of Pediococcus acidilactici (BE and BK) on gastric mucin is in accordance with the presence of ef-Tu and mub genes. High level attachment in lactobacilli is likely to correlate with the ef-Tu gene, which is a lactobacilli-specifc adhesive gene.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2015-12-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/24346</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.24346</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 20, No 2 (2015); 174-181</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/24346/15839</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2017 Indonesian Journal of Biotechnology</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>