Melola Discovery

← Melola Discovery

Open access Journal SINTA 1

Evaluation of rapid detection kit against avian influenza A virus and H5 subtype for field Sample

Indonesian Journal of Biotechnology · Vol. 21 No. 1 (2016) · pp. 48–55

DOI
10.22146/ijbiotech.26792
ISSN
2089-2241, 0853-8654
Published
2016-06-01
Publisher
Universitas Gadjah Mada
Language
eng
License
creativecommons.org/licenses/by-sa/4.0

Abstract

Avian influenza virus is poultry viral disease, which causes high economic losses. Various efforts have been made to control the disease. One effort is required screening fast and precise diagnostic test. This study was aimed to determine the potential of rapid test kit of AIV/H5 Anigen Rapid Test for the detection of AI virus types A and subtype H5 in the field. Some tests were carried out, e.g.: the potential test, cross-reaction test, sensitivity and specificity test. Potency test was done to evaluate potential of detection limits of the kit, by having the test of serial dilution of AI virus positive control. Cross-reaction test was done to detect antigens other than AI virus H5N1, e.g.: IB virus of Massachuset strain, IBV strain 4-91, Newcastle Disease virus, and Escherichia coli. Sensitivity and specificity test were applied to the filed samples which clinically and laboratory were confirmed as H5N1 positive. To confirm the result of rapid test was then being done by reverse transcriptase polymerase chain reaction. Based on these results it can be concluded that, Anigen Kit AIV/H5 Ag Rapid Test can detect antigen-containing samples having AI virus HA titer up to 26of type A virus, and up to 25 for subtype H5 virus. Anigen Kit AIV/H5 Ag Rapid Test showed no cross-reactions with Infectious Bronchitis virus, Newcastle Disease virus, and Escherichia coli. Anigen A Rapid Test Kit AIV Ag showed a sensitivity of 50% and specificity of 100%, while Anigen Ag Rapid Test Kit AIV/H5 showed a sensitivity of 25% and specificity is 100%.

Keywords

Collaboration network of the authors

40 authors · 98 co-authorship links · 4 across different organisations · showing the 40 most connected

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • Universitas Gadjah Mada
  • Sriwijaya University
  • Universitas Negeri Surabaya
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Khrisdiana PutriAffiliation unknown4
Wayan T. ArtamaAffiliation unknown3
Yuli Purwandari KristianingrumAffiliation unknown3
Charles Rangga TabbuAffiliation unknown3
Arum SetiawanSriwijaya University2
Mammed SagiUniversitas Gadjah Mada2
Istriyati IstriyatiUniversitas Gadjah Mada2
Marsetyawan HNE SoesatyoAffiliation unknown2
Jaka WidadaUniversitas Gadjah Mada2
M. MustofaAffiliation unknown2
A. AlimuddinAffiliation unknown2
SEBASTIAN MARGINOAffiliation unknown2
W. WidodoUniversitas Negeri Surabaya2
Sri DarmawatiAffiliation unknown2
LANGKAH SEMBIRINGAffiliation unknown2
Heru SusetyaAffiliation unknown2
Sitarina WidyariniAffiliation unknown2
Pinandi Sri PudyaniAffiliation unknown1
Ika Dewi AnaAffiliation unknown1
Tita Ratya UtariAffiliation unknown1
Siti SunarintyasAffiliation unknown1
Juni HandajaniAffiliation unknown1
Yulita KristantiAffiliation unknown1
Risya CilmiatyAffiliation unknown1
Mandojo RukmoAffiliation unknown1
Afiono Agung PrasetyoAffiliation unknown1
Khilyat Ulin Nur ZainiAffiliation unknown1
Suhartono Taat PutraAffiliation unknown1
Banun KusumawardaniAffiliation unknown1
Djaswadi DasukiAffiliation unknown1
Al. Supartinah SantosoAffiliation unknown1
Regina TC. TandelilinAffiliation unknown1
Abdul Salam M. SofroAffiliation unknown1
Dwi Aji NugrohoAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Evaluation of rapid detection kit against avian influenza A virus and H5 subtype for field Sample</dc:title>
	<dc:creator>Wibowo, Michael Haryadi</dc:creator>
	<dc:creator>Untari, Tri</dc:creator>
	<dc:creator>Artanto, Sidna</dc:creator>
	<dc:creator>Putri, Krisdiana</dc:creator>
	<dc:creator>Amanu, Surya</dc:creator>
	<dc:creator>Asmara, Widya</dc:creator>
	<dc:subject xml:lang="en-US">avian influenza; rapid detection kit; specificity and sensitivity</dc:subject>
	<dc:description xml:lang="en-US">Avian influenza virus is poultry viral disease, which causes high economic losses. Various efforts have been made to control the disease. One effort is required screening fast and precise diagnostic test. This study was aimed to determine the potential of rapid test kit of AIV/H5 Anigen Rapid Test for the detection of AI virus types A and subtype H5 in the field. Some tests were carried out, e.g.: the potential test, cross-reaction test, sensitivity and specificity test. Potency test was done to evaluate potential of detection limits of the kit, by having the test of serial dilution of AI virus positive control. Cross-reaction test was done to detect antigens other than AI virus H5N1, e.g.:  IB virus of Massachuset strain, IBV strain 4-91, Newcastle Disease virus, and Escherichia coli. Sensitivity and specificity test were applied to the filed samples which clinically and laboratory were confirmed as H5N1 positive. To confirm the result of rapid test was then being done by reverse transcriptase polymerase chain reaction. Based on these results it can be concluded that, Anigen Kit AIV/H5 Ag Rapid Test can detect antigen-containing samples having AI virus HA titer up to 26of type A virus, and up to 25 for subtype H5 virus. Anigen Kit AIV/H5 Ag Rapid Test showed no cross-reactions with Infectious Bronchitis virus, Newcastle Disease virus, and Escherichia coli. Anigen A Rapid Test Kit AIV Ag showed a sensitivity of 50% and specificity of 100%, while Anigen Ag Rapid Test Kit AIV/H5 showed a sensitivity of 25% and specificity is 100%.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2016-06-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/26792</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.26792</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 21, No 1 (2016); 48-55</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/26792/16539</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2016 The Author(s)</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>