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The establishment of PCR amplification, cloning, and sequencing of bovine herpesvirus 1 (BHV-1) glycoprotein D gene isolated in Indonesia

Indonesian Journal of Biotechnology · Vol. 24 No. 1 (2019) · pp. 34–42

DOI
10.22146/ijbiotech.44298
ISSN
2089-2241, 0853-8654
Published
2019-06-18
Publisher
Universitas Gadjah Mada
Language
eng
License
creativecommons.org/licenses/by-sa/4.0

Abstract

Considering the increasing incidence of infectious bovine rhinotracheitis (IBR) in Indonesia, it was necessary to conduct a more in-depth study of bovine herpesvirus-1 (BHV-1) as the causative agent of IBR disease. Previous research reports indicate that the BHV-1 subtypes found in Indonesia are subtype 1.1. Currently, IBR field case detection in Indonesia still uses the serological method (ELISA), which has the potential to give false positive results and cannot explain the virus subtype. Other detection methods, such as viral isolation, take longer and require adequate resources. This study aimed to determine the BHV-1 subtypes of Indonesian isolates using molecular techniques. Nested PCR using two pairs of primers was successfully used to amplify the glycoprotein D (gD) gene. The gD gene fragment was cloned into the pGEM-T plasmid. Analysis of the gD gene sequence was subsequently carried out to determine the BHV-1 character of the Indonesian isolates. The results indicated that the isolates were different from the previous isolates, and had similarities (100%) with subtype 1.2 strain SP1777 and SM023.

Keywords

Collaboration network of the authors

40 authors · 98 co-authorship links · 4 across different organisations · showing the 40 most connected

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • Universitas Gadjah Mada
  • Sriwijaya University
  • Universitas Negeri Surabaya
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Khrisdiana PutriAffiliation unknown4
Wayan T. ArtamaAffiliation unknown3
Yuli Purwandari KristianingrumAffiliation unknown3
Charles Rangga TabbuAffiliation unknown3
Arum SetiawanSriwijaya University2
Mammed SagiUniversitas Gadjah Mada2
Istriyati IstriyatiUniversitas Gadjah Mada2
Marsetyawan HNE SoesatyoAffiliation unknown2
Jaka WidadaUniversitas Gadjah Mada2
M. MustofaAffiliation unknown2
A. AlimuddinAffiliation unknown2
SEBASTIAN MARGINOAffiliation unknown2
W. WidodoUniversitas Negeri Surabaya2
Sri DarmawatiAffiliation unknown2
LANGKAH SEMBIRINGAffiliation unknown2
Heru SusetyaAffiliation unknown2
Sitarina WidyariniAffiliation unknown2
Pinandi Sri PudyaniAffiliation unknown1
Ika Dewi AnaAffiliation unknown1
Tita Ratya UtariAffiliation unknown1
Siti SunarintyasAffiliation unknown1
Juni HandajaniAffiliation unknown1
Yulita KristantiAffiliation unknown1
Risya CilmiatyAffiliation unknown1
Mandojo RukmoAffiliation unknown1
Afiono Agung PrasetyoAffiliation unknown1
Khilyat Ulin Nur ZainiAffiliation unknown1
Suhartono Taat PutraAffiliation unknown1
Banun KusumawardaniAffiliation unknown1
Djaswadi DasukiAffiliation unknown1
Al. Supartinah SantosoAffiliation unknown1
Regina TC. TandelilinAffiliation unknown1
Abdul Salam M. SofroAffiliation unknown1
Dwi Aji NugrohoAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en-US">The establishment of PCR amplification, cloning, and sequencing of bovine herpesvirus 1 (BHV-1)  glycoprotein D gene isolated in Indonesia</dc:title>
	<dc:creator>Hidayati, Dewi Noor</dc:creator>
	<dc:creator>Srihanto, Eko Agus</dc:creator>
	<dc:creator>Untari, Tri</dc:creator>
	<dc:creator>Wibowo, Michael Haryadi</dc:creator>
	<dc:creator>Akiyama, Koichi</dc:creator>
	<dc:creator>Asmara, Widya</dc:creator>
	<dc:subject xml:lang="en-US">bovine herpesvirus-1 (BHV-1); glycoprotein D; Indonesia; infectious bovine rhinotracheitis (IBR); subtypes</dc:subject>
	<dc:description xml:lang="en-US">Considering the increasing incidence of infectious bovine rhinotracheitis (IBR) in Indonesia, it was necessary to conduct a more in-depth study of bovine herpesvirus-1 (BHV-1) as the causative agent of IBR disease. Previous research reports indicate that the BHV-1 subtypes found in Indonesia are subtype 1.1. Currently, IBR field case detection in Indonesia still uses the serological method (ELISA), which has the potential to give false positive results and cannot explain the virus subtype. Other detection methods, such as viral isolation, take longer and require adequate resources. This study aimed to determine the BHV-1 subtypes of Indonesian isolates using molecular techniques. Nested PCR using two pairs of primers was successfully used to amplify the glycoprotein D (gD) gene. The gD gene fragment was cloned into the pGEM-T plasmid. Analysis of the gD gene sequence was subsequently carried out to determine the BHV-1 character of the Indonesian isolates. The results indicated that the isolates were different from the previous isolates, and had similarities (100%) with subtype 1.2 strain SP1777 and SM023.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US">Japan Student Service Organization</dc:contributor>
	<dc:date>2019-06-18</dc:date>
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	<dc:identifier>10.22146/ijbiotech.44298</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 24, No 1 (2019); 34-42</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
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	<dc:rights xml:lang="en-US">Copyright (c) 2019 The Author(s)</dc:rights>
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