Melola Discovery

← Melola Discovery

Open access Journal SINTA 1

Cytoprotective activity of carrot and tomato callus extracts and the ex‐ pression of cytokines in UV‐B irradiated fibroblast cells

Indonesian Journal of Biotechnology · Vol. 24 No. 2 (2020) · pp. 94–100

DOI
10.22146/ijbiotech.51734
ISSN
2089-2241, 0853-8654
Published
2020-01-02
Publisher
Universitas Gadjah Mada
Language
eng
License
creativecommons.org/licenses/by-sa/4.0

Abstract

Studies have suggested that both carrot (Daucus carota L.) and tomato (Solanum lycopersicum L.) callus extracts contain antoxidant compounds that might have the potental to protect cells from free radicals such as H2O2 that contribute to cell damage. The other sources of free radical exposure in human cells, such as UV‐B, should also be examined. UV‐B exposure can trigger increased expression of inflammatory cytokines such as cyclooxygenase‐2 (COX‐2) and tumor necrosis factor‐α (TNF‐α) and the antinflammatory cytokine interleukin‐10 (IL‐10), which causes photoaging. This study was conducted to investigate the cytoprotectve actvity of carrot and tomato callus aqueous extracts by observing cell viability using the MTT assay. Immunocytochemistry methods were used to examine the effects of carrot and tomato callus aqueous extracts on the expression of COX‐2, TNF‐α, and IL‐10 in human dermal fibroblast adult (HDFa) cells exposed to UV‐B light. Carrot and tomato callus aqueous extracts were obtained by the maceration method using aqua bidistilled solvent. Results showed that both carrot and tomato callus aqueous extracts at 0.5 mg/mL exhibited the highest cytoprotective effect in HDFa cells compared to that at other concentratons. Both carrot and tomato callus aqueous extracts could also decrease the expression of COX‐2 and TNF‐α, whereas carrot callus aqueous extract increased the expression of the anti‐inflammatory cytokine IL‐10 in HDFa cells.

Keywords

Collaboration network of the authors

40 authors · 115 co-authorship links · showing the 40 most connected

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • Universitas Gadjah Mada
  • Cenderawasih University
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Aziz PurwantoroAffiliation unknown11
Sukarti MoeljopawiroAffiliation unknown6
Ari IndriantoAffiliation unknown4
Muhammad Dylan LawrieAffiliation unknown4
Windi MoseAffiliation unknown3
Febri Yuda KurniawanAffiliation unknown3
Rarastoeti PratiwiAffiliation unknown3
Jose Gutierrez-MarcosAffiliation unknown3
Nintya SetiariAffiliation unknown2
Jaka WidadaUniversitas Gadjah Mada2
Aviesta LinggabuwanaAffiliation unknown2
Saifa Usni PutriAffiliation unknown2
Nuzlan RasjidAffiliation unknown2
Exsyupransia MursyantiAffiliation unknown2
Fitriana PuspitasariAffiliation unknown2
Riris Istighfari JenieAffiliation unknown2
Aries Bagus SasongkoUniversitas Gadjah Mada2
Naufal Ghozi Aditya PerdanaAffiliation unknown2
Dika SundariAffiliation unknown2
Yekti Asih PurwestriAffiliation unknown2
Woro Anindito Sri TunjungAffiliation unknown2
Aditya Krishar KarimCenderawasih University1
Khamidun KhamidunAffiliation unknown1
Tri Joko RaharjoAffiliation unknown1
Stalis Norma EthicaAffiliation unknown1
Oedjijono OedjijonoAffiliation unknown1
Triono Bagus SaputroAffiliation unknown1
Zulfa LayinaAffiliation unknown1
Della Rosiana NingtiasAffiliation unknown1
Falah Nur AlifiantoAffiliation unknown1
Budi Setiadi DaryonoAffiliation unknown1
Rina ArimarsetiowatiAffiliation unknown1
Yohana Theresia Maria AstutiAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Cytoprotective activity of carrot and tomato callus extracts and the ex‐ pression of cytokines in UV‐B irradiated fibroblast cells</dc:title>
	<dc:creator>Rumiyati, Rumiyati</dc:creator>
	<dc:creator>Sismindari, Sismindari</dc:creator>
	<dc:creator>Semiarti, Endang</dc:creator>
	<dc:creator>Widyarani, Sitarina</dc:creator>
	<dc:creator>Sari, Dewi Tika</dc:creator>
	<dc:creator>Apritadila, Brilliant Kharisma</dc:creator>
	<dc:creator>Riastri, Anami</dc:creator>
	<dc:subject xml:lang="en-US">carrot (Daucus carota L.) and tomato (Solanum lycopersicum L.) callus aqueous extract; viability cell; cyclooxygenase‐2 (COX‐2); tumor necrosis factor‐α (TNF‐α); interleukin‐10 (IL‐10)</dc:subject>
	<dc:description xml:lang="en-US">Studies have suggested that both carrot (Daucus carota L.) and tomato (Solanum lycopersicum L.) callus extracts contain antoxidant compounds that might have the potental to protect cells from free radicals such as H2O2 that contribute to cell damage. The other sources of free radical exposure in human cells, such as UV‐B, should also be examined. UV‐B exposure can trigger increased expression of inflammatory cytokines such as cyclooxygenase‐2 (COX‐2) and tumor necrosis factor‐α (TNF‐α) and the antinflammatory cytokine interleukin‐10 (IL‐10), which causes photoaging. This study was conducted to investigate the cytoprotectve actvity of carrot and tomato callus aqueous extracts by observing cell viability using the MTT assay. Immunocytochemistry methods were used to examine the effects of carrot and tomato callus aqueous extracts on the expression of COX‐2, TNF‐α, and IL‐10 in human dermal fibroblast adult (HDFa) cells exposed to UV‐B light. Carrot and tomato callus aqueous extracts were obtained by the maceration method using aqua bidistilled solvent. Results showed that both carrot and tomato callus aqueous extracts at 0.5 mg/mL exhibited the highest cytoprotective effect in HDFa cells compared to that at other concentratons. Both carrot and tomato callus aqueous extracts could also decrease the expression of COX‐2 and TNF‐α, whereas carrot callus aqueous extract increased the expression of the anti‐inflammatory cytokine IL‐10 in HDFa cells. </dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2020-01-02</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/51734</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.51734</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 24, No 2 (2019); 94-100</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/51734/26700</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/51734/10112</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/51734/10113</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/51734/10114</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/51734/10115</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/51734/10116</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/downloadSuppFile/51734/10117</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2020 The Author(s)</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>