A novel pilot bioreactor for scaling up biomass and bioactive compounds on Gynura procumbens adventitious root culture

Kusuma, Dannis Yuda; Kristanti, Alfinda Novi; Manuhara, Yosephine Sri Wulan

doi:10.22146/ijbiotech.78420

Open access Journal SINTA 1

A novel pilot bioreactor for scaling up biomass and bioactive compounds on Gynura procumbens adventitious root culture

Indonesian Journal of Biotechnology · Vol. 28 No. 2 (2023) · pp. 66–76

DOI: 10.22146/ijbiotech.78420
ISSN: 2089-2241, 0853-8654
Published: 2023-06-27
Publisher: Universitas Gadjah Mada
Language: eng
License: creativecommons.org/licenses/by-sa/4.0

Abstract

Bioreactors for adventitious root culture have been developed to obtain biomass and plant bioactive compounds in large quantities. These technologies provide a great opportunity to produce biomass and bioactive compounds more quickly from Gynura procumbens compared to conventional plant cultivation systems. In previous studies, biomass and bioactive compounds of G. procumbens adventitious roots were successfully increased using a small‐scale bioreactor. In this study, a pilot bioreactor the capacity of 19 L polycarbonate gallon was successfully developed. This bioreactor can be sterilized under the pressure of 0.18 MPa for approximately 60 min. While the bioreactor could not be sterilized when the pressure was less than 0.18 MPa damage may have occurred to the bioreactor vessel at pressures exceeding 0.18 MPa. The results of the chemical grade test as root culture media showed that MS‐Tek provided an optimal root biomass compared to MS‐PA after a 35‐day of the culture period. In addition, the productivity of the total phenolics and flavonoids of adventitious root in MS‐PA was higher than in MS‐Tek. This novel pilot bioreactor is suitable for G. procumbens adventitious root culture, and the technical‐grade chemicals are suitable for improving root biomass production.

Keywords

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23 authors · 73 co-authorship links

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Co-author	Organisation	Joint articles
Anjar Tri Wibowo	Affiliation unknown	5
Boon Chin Tan	Affiliation unknown	4
Arif Yachya	Affiliation unknown	4
Sugiharto -	Affiliation unknown	2
Dwi Winarni	Affiliation unknown	2
Ufairanisa Islamatasya	Affiliation unknown	2
Nindi Novia Erin	Affiliation unknown	2
Djarot Sugiarso	Affiliation unknown	2
Gebby Agnessya Esa Oktavia	Affiliation unknown	1
Junairiah Junairiah	Affiliation unknown	1
Palupi Dasawulan Lestari	Affiliation unknown	1
Syifa Fajrisani	Affiliation unknown	1
Putri Gehasti	Affiliation unknown	1
Nur Afni Jayanti	Affiliation unknown	1
Angelia Krisdanti Nasera Putri	Affiliation unknown	1
Nabilatun Nisa’	Affiliation unknown	1
Idqa Nurtri Bhakti	Affiliation unknown	1
Sugiharto Sugiharto	Affiliation unknown	1
Abdul Hakim Muhsyi	Affiliation unknown	1
Ahimsa Buena Merpati	Affiliation unknown	1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.

Original metadata (oai_dc)

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	<dc:title xml:lang="en-US">A novel pilot bioreactor for scaling up biomass and bioactive compounds on Gynura procumbens adventitious root culture</dc:title>
	<dc:creator>Kusuma, Dannis Yuda</dc:creator>
	<dc:creator>Kristanti, Alfinda Novi</dc:creator>
	<dc:creator>Manuhara, Yosephine Sri Wulan</dc:creator>
	<dc:subject xml:lang="en-US">Adventitious root; Bioactive compound; Chemical grade; G. procumbens; Pilot bioreactor</dc:subject>
	<dc:description xml:lang="en-US">Bioreactors for adventitious root culture have been developed to obtain biomass and plant bioactive compounds in large quantities. These technologies provide a great opportunity to produce biomass and bioactive compounds more quickly from Gynura procumbens compared to conventional plant cultivation systems. In previous studies, biomass and bioactive compounds of G. procumbens adventitious roots were successfully increased using a small‐scale bioreactor. In this study, a pilot bioreactor the capacity of 19 L polycarbonate gallon was successfully developed. This bioreactor can be sterilized under the pressure of 0.18 MPa for approximately 60 min. While the bioreactor could not be sterilized when the pressure was less than 0.18 MPa damage may have occurred to the bioreactor vessel at pressures exceeding 0.18 MPa. The results of the chemical grade test as root culture media showed that MS‐Tek provided an optimal root biomass compared to MS‐PA after a 35‐day of the culture period. In addition, the productivity of the total phenolics and flavonoids of adventitious root in MS‐PA was higher than in MS‐Tek. This novel pilot bioreactor is suitable for G. procumbens adventitious root culture, and the technical‐grade chemicals are suitable for improving root biomass production.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US">Universitas Airlangga</dc:contributor>
	<dc:date>2023-06-27</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/78420</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.78420</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 28, No 2 (2023); 66-76</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/78420/36785</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/78420/36784</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2023 The Author(s)</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
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