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INFLUENCE OF CULTURE MEDIUM ON THE SPORULATION AND VIABILITY OF ASPERGILLUS SPP. AND TALAROMYCES SPP. ENTOMOPATHOGENIC FUNGI

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA · Vol. 18 No. 1 (2018) · pp. 12–22

DOI
10.23960/j.hptt.11812-22
ISSN
2461-0399, 1411-7525
Published
2018-08-02
Publisher
Universitas Lampung
Language
eng
License
creativecommons.org/licenses/by-nc/4.0

Abstract

Influence of Culture Medium on the Sporulation and Viability of Aspergillus spp. and Talaromyces spp. Entomopathogenic Fungi. The purpose of this study was to determine the effect of three kinds of cultures media on the spore production and viability of Aspergillus spp. (AS1, 6, 7, 9) and Talaromyces spp. (AS2–5, 8, 10) entomopathogenic fungi. This study was arranged using Factorial-Completely Randomized Design (CRD) with 2 factors and 3 replications. The first factor was three kinds of cultures media (potato dextrose agar (PDA), corn meal agar (CMA), and sabouraud dextrose agar (SDA)) and the second one was isolates of Aspergillus spp. Or Talaromyces spp.. Data of spore production and spore viability were tested using ANOVA and if there was significantly difference, the data then further analyzed using Tukey‘s Honestly Significant Difference (HSD) test at 5% of significant level. The spore production of Aspergillus spp. were in the range of 0.58 - 14.27 x 108 spores mL-1 (PDA); 0.28 – 2.68 x 108 spores mL-1 (SDA) and 1.85 - 5.33 x 108 spores mL-1 (CMA). The highest spore production was achieved by AS1 isolate that was grown on PDA media. The spore produced by Talaromyces spp. were in the range of 2.15 – 28.62 x108 spores mL-1 (PDA); 0.28 – 29.43 x108 spores mL-1 (SDA); and 1.88 – 16.63 x108 spores mL-1 (CMA). The highest spore production was produced by AS8 isolate which were cultured on PDA. The spore viability among isolates of the two entomopathogenic fungi were not significantly different. The spore viability of Aspergillus spp. was in the range of 95.10 – 97.66% (PDA), 94.02 – 98.45% (SDA) and 92.86 – 98.20% (CMA). The spore viability of Talaromyces spp. was in the range of 95.83 – 100% (PDA), 85.83 – 100% (SDA), and 90.75 – 100% (CMA). Culture medium influenced spore production but not the spore viability. The best culture media used for spore production of both of the entomopathogenic fungi was PDA media.

Keywords

Collaboration network of the authors

40 authors · 143 co-authorship links · 1 across different organisations · showing the 40 most connected

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  • Jenderal Soedirman University
  • Universitas Pembangunan Nasional Veteran Yogyakarta
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
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Co-authorOrganisationJoint articles
Lestari WibowoAffiliation unknown12
Franciscus Xaverius SusiloAffiliation unknown7
Agus M. HaririAffiliation unknown7
Rosma HasibuanAffiliation unknown6
Setyo Dwi UtomoAffiliation unknown6
, IndriyatiAffiliation unknown5
Hamim SudarsonoAffiliation unknown5
Solikhin .Affiliation unknown5
Agus Muhammad HaririAffiliation unknown5
Erisandi ArditamaAffiliation unknown4
Nur PrihatiningsihAffiliation unknown3
Titik Nur AenyAffiliation unknown3
Eko Teguh ParipurnoUniversitas Pembangunan Nasional Veteran Yogyakarta2
Yozi Aulia RahmanAffiliation unknown2
Ahmad Syahrul FauziAffiliation unknown2
M Fikri AmrullahAffiliation unknown2
Arvangga Oktadian KrisbiyantoroAffiliation unknown2
Moh Aris MunandarAffiliation unknown2
Tri Marhaeni Pudji AstutiAffiliation unknown2
Pradipta KurniasantiAffiliation unknown2
Martien Herna SusantiAffiliation unknown2
Sunarto SunartoAffiliation unknown2
Eko HandoyoAffiliation unknown2
Rerenstradika Tizar TerryanaAffiliation unknown2
Kristianto NugrohoAffiliation unknown2
HEE JONG KOHAffiliation unknown2
Araz MeilinAffiliation unknown2
Yulia PujiastutiAffiliation unknown2
Jauharlina JauharlinaAffiliation unknown2
Tri AtmowidiAffiliation unknown2
RIKA RAFFIUDINAffiliation unknown2
Windra PriawandiputraAffiliation unknown2
Mahardika Gama PradanaAffiliation unknown2
Jasmi JasmiAffiliation unknown2

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) http://jhpttropika.fp.unila.ac.id/index.php/jhpttropika/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en">INFLUENCE OF CULTURE MEDIUM ON THE SPORULATION AND VIABILITY OF ASPERGILLUS SPP. AND TALAROMYCES SPP. ENTOMOPATHOGENIC FUNGI</dc:title>
	<dc:creator xml:lang="en">Fitriana, Yuyun</dc:creator>
	<dc:creator xml:lang="en">Suharjo, Radix</dc:creator>
	<dc:creator xml:lang="en">Swibawa, I Gede</dc:creator>
	<dc:creator xml:lang="en">., Purnomo</dc:creator>
	<dc:creator xml:lang="en">Lestari, Puji</dc:creator>
	<dc:creator xml:lang="en">Merdiana, Eryka</dc:creator>
	<dc:subject xml:lang="en">culture medium</dc:subject>
	<dc:subject xml:lang="en">Aspergillus spp.</dc:subject>
	<dc:subject xml:lang="en">Talaromyces spp.</dc:subject>
	<dc:subject xml:lang="en">entomopathogenic fungi</dc:subject>
	<dc:subject xml:lang="en">spore production and viability</dc:subject>
	<dc:description xml:lang="en">Influence of Culture Medium on the Sporulation and Viability of Aspergillus spp. and Talaromyces spp. Entomopathogenic Fungi. The purpose of this study was to determine the effect of three kinds of cultures media on the spore production and viability of Aspergillus spp. (AS1, 6, 7, 9) and Talaromyces spp. (AS2–5, 8, 10) entomopathogenic fungi. This study was arranged using Factorial-Completely Randomized Design (CRD) with 2 factors and 3 replications. The first factor was three kinds of cultures media (potato dextrose agar (PDA), corn meal agar (CMA), and sabouraud dextrose agar (SDA)) and the second one was isolates of Aspergillus spp. Or Talaromyces spp.. Data of spore production and spore viability were tested using ANOVA and if there was significantly difference, the data then further analyzed using Tukey‘s Honestly Significant Difference (HSD) test at 5% of significant level. The spore production of Aspergillus spp. were in the range of 0.58 - 14.27 x 108 spores mL-1 (PDA); 0.28 – 2.68 x 108 spores mL-1 (SDA) and 1.85 - 5.33 x 108 spores mL-1 (CMA). The highest spore production was achieved by AS1 isolate that was grown on PDA media. The spore produced by Talaromyces spp. were in the range of 2.15 – 28.62 x108 spores mL-1 (PDA); 0.28 – 29.43 x108 spores mL-1 (SDA); and 1.88 – 16.63 x108 spores mL-1 (CMA). The highest spore production was produced by AS8 isolate which were cultured on PDA. The spore viability among isolates of the two entomopathogenic fungi were not significantly different. The spore viability of Aspergillus spp. was in the range of 95.10 – 97.66% (PDA), 94.02 – 98.45% (SDA) and 92.86 – 98.20% (CMA). The spore viability of Talaromyces spp. was in the range of 95.83 – 100% (PDA), 85.83 – 100% (SDA), and 90.75 – 100% (CMA). Culture medium influenced spore production but not the spore viability. The best culture media used for spore production of both of the entomopathogenic fungi was PDA media.</dc:description>
	<dc:publisher xml:lang="en">Universitas Lampung</dc:publisher>
	<dc:date>2018-08-02</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
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	<dc:identifier>https://jhpttropika.fp.unila.ac.id/index.php/jhpttropika/article/view/457</dc:identifier>
	<dc:identifier>10.23960/j.hptt.11812-22</dc:identifier>
	<dc:source xml:lang="en">Jurnal Hama dan Penyakit Tumbuhan Tropika; Vol. 18 No. 1 (2018): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA; 12-22</dc:source>
	<dc:source>2461-0399</dc:source>
	<dc:source>1411-7525</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://jhpttropika.fp.unila.ac.id/index.php/jhpttropika/article/view/457/pdf</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2018 Jurnal Hama dan Penyakit Tumbuhan Tropika</dc:rights>
	<dc:rights xml:lang="en">https://creativecommons.org/licenses/by-nc/4.0</dc:rights>
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