Melola Discovery

← Melola Discovery

Open access Journal SINTA 1

Morphological and molecular identification as well pathogenicity of the causal agents of fruit rot disease of apple manalagi (Malus sylvestris) in Pujon, East Java

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA · Vol. 23 No. 1 (2023) · pp. 56–66

DOI
10.23960/jhptt.12356-66
ISSN
2461-0399, 1411-7525
Published
2023-01-25
Publisher
Universitas Lampung
Language
eng
License
creativecommons.org/licenses/by-nc/4.0

Abstract

Apple fruit rot disease is one of the major apple diseases in Indonesia. It has been caused by several species of pathogenic within the genus Colletotrichum. This research aims to identify species of the pathogen causing apple fruit rot disease in Pujon, East Java. Suspected fungal isolates were identified based on morphological (macroscopic and microscopic) and molecular characteristics. Based on the morphological observation, five isolates were identified as Colletotrichum spp. These isolates have similar morphological characteristics such as white-greyish colony color, texture colony velvety, zonation conidia concentric, round cylindrical conidial end, and conidia with 10.4–12.8?m in length and width 3.1–3.52?m, respectively. Isolates M1 showed the highest pathogenicity, therefore selected for molecular identification. Molecular identification was conducted using ITS1 and ITS4 primer, AM1 was identified as C. gloeosporioides with 99.57% similarity to C. gloeosporioides JX-19 variety from China.

Keywords

Collaboration network of the authors

40 authors · 124 co-authorship links · showing the 40 most connected

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • University of Brawijaya
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Luqman Qurata AiniAffiliation unknown7
Hagus TarnoAffiliation unknown4
Gatot MudjionoAffiliation unknown3
Sri KarindahAffiliation unknown2
Ardiyan TarunaAffiliation unknown2
Rifani Rusiana DewiAffiliation unknown2
Muhammad Akhid Syib’liAffiliation unknown2
Toto HimawanAffiliation unknown2
Silvi IkawatiAffiliation unknown2
Ariffin Noor SugihartoAffiliation unknown2
Lilis SuryaniAffiliation unknown2
Moh. Hibban ToanaAffiliation unknown1
Amalia Khairunnisa’Affiliation unknown1
Erekso HadiwijoyoAffiliation unknown1
Izmi YulianahAffiliation unknown1
Anton MuhibuddinAffiliation unknown1
Luaili AddinaAffiliation unknown1
Athoillah AhmadAffiliation unknown1
Cokorda JavandiraAffiliation unknown1
Ito FernandoUniversity of Brawijaya1
Syarifah Maulidya RahmahAffiliation unknown1
Arifin Noor SugihartoAffiliation unknown1
Syamsuddin DjauhariAffiliation unknown1
Wiwit Wicaksono JatiAffiliation unknown1
Wita FirdausiAffiliation unknown1
Mintarto MartosudiroAffiliation unknown1
Rina RachmawatiAffiliation unknown1
Akhmad RizaliAffiliation unknown1
Muhammad FebriansyahAffiliation unknown1
Theresia Rani Kartika AyuAffiliation unknown1
Moch. Dawam MaghfoerAffiliation unknown1
Tatik WardiyatiAffiliation unknown1
Anis RosyidahAffiliation unknown1
Samharinto SamharintoAffiliation unknown1
Bambang Tri RaharjoAffiliation unknown1
Hakimah HalimAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) http://jhpttropika.fp.unila.ac.id/index.php/jhpttropika/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Morphological and molecular identification as well pathogenicity of the causal agents of fruit rot disease of apple manalagi (Malus sylvestris) in Pujon, East Java</dc:title>
	<dc:creator xml:lang="en">Triasih, Unun </dc:creator>
	<dc:creator xml:lang="en">Abadi, Abdul Latief </dc:creator>
	<dc:creator xml:lang="en">Muhibbudin, Anton </dc:creator>
	<dc:creator xml:lang="en">Widyaningsih, Sri </dc:creator>
	<dc:subject xml:lang="en">apple</dc:subject>
	<dc:subject xml:lang="en">Colletotrichum</dc:subject>
	<dc:subject xml:lang="en">fruit rot</dc:subject>
	<dc:subject xml:lang="en">pathogenicity</dc:subject>
	<dc:description xml:lang="en">Apple fruit rot disease is one of the major apple diseases in Indonesia. It has been caused by several species of pathogenic within the genus Colletotrichum. This research aims to identify species of the pathogen causing apple fruit rot disease in Pujon, East Java. Suspected fungal isolates were identified based on morphological (macroscopic and microscopic) and molecular characteristics. Based on the morphological observation, five isolates were identified as Colletotrichum spp. These isolates have similar morphological characteristics such as white-greyish colony color, texture colony velvety, zonation conidia concentric, round cylindrical conidial end, and conidia with 10.4–12.8 ?m in length and width 3.1–3.52 ?m, respectively. Isolates M1 showed the highest pathogenicity, therefore selected for molecular identification. Molecular identification was conducted using ITS1 and ITS4 primer, AM1 was identified as C. gloeosporioides with 99.57% similarity to C. gloeosporioides JX-19 variety from China.</dc:description>
	<dc:publisher xml:lang="en">Universitas Lampung</dc:publisher>
	<dc:date>2023-01-25</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://jhpttropika.fp.unila.ac.id/index.php/jhpttropika/article/view/635</dc:identifier>
	<dc:identifier>10.23960/jhptt.12356-66</dc:identifier>
	<dc:source xml:lang="en">Jurnal Hama dan Penyakit Tumbuhan Tropika; Vol. 23 No. 1 (2023): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PESTS AND DISEASES; 56-66</dc:source>
	<dc:source>2461-0399</dc:source>
	<dc:source>1411-7525</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://jhpttropika.fp.unila.ac.id/index.php/jhpttropika/article/view/635/546</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2023 Jurnal Hama dan Penyakit Tumbuhan Tropika</dc:rights>
	<dc:rights xml:lang="en">https://creativecommons.org/licenses/by-nc/4.0</dc:rights>
</oai_dc:dc>