Antagonistic feature displayed by endophytic bacteria consortium for control rice pathogens

Abstract

Rhizoctonia solani and Xanthomonas oryzae pv. oryzae (Xoo) are important pathogens causing severe damage on rice. It makes severe damage and is required for the management of the disease. One of the management of the disease is the use of endophytic bacteria. The purpose of this study was to determine the compatibility of endophytic bacteria as consortium, to examine the potency of the endophytic bacteria consortium against R. solani and Xoo and to understand the mechanism of inhibition of endophytic bacteria consortium against R. solani and Xoo. Assessment on the compatibility between endophytic bacteria was performed in a petridish using streak method. The endophytic bacterial consortium antagonism test against rice pathogenic fungi and bacteria was carried out in a completely randomized design with 2 treatments and 15 replications. The variables observed were the compatibility of endophytic bacteria, the percentage of inhibition and the zone of inhibition. The mechanism of inhibition was observed from changes in the form of pathogenic fungi and bacteriostatic or bactericidal. The results showed that among 12 compatible endophytic bacteria as constituents of the consortium, five isolates of endophytic bacteria showed strong and very strong growth with the other isolates. These isolates from Petanahan Kebumen, Karangwangkal Purwokerto and Sumbang Banyumas. The consortium of endophytic bacteria was able to suppress the growth of R. solani by 57.14% with antibiosis mechanism causing swelling of the mycelium and formed a red pigment. The endophytic bacteria consortium inhibited the growth of Xoo by 15 mm with a bacteriostatic antibiosis mechanism. Consortium endophytic bacteria can be used as an alternative to control plant diseases which also has the opportunity to be formulated and applied to plants.