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Screening of entomopathogenic and endophytic fungi from various landscapes and evaluation of their antagonistic activity against Fusarium sp.

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA · Vol. 26 No. 2 (2026) · pp. 572–588

DOI
10.23960/j.hptt.226572-588
ISSN
2461-0399, 1411-7525
Published
2026-09-17
Publisher
Universitas Lampung
Language
eng
License
creativecommons.org/licenses/by-nc/4.0

Abstract

Forestry research is increasingly focused on exploring microbial resources, including entomopathogenic endophytic fungi (EEFs), as potential biological control agent. This study aimed to explore and characterize fungal isolates obtained from different landscapes and biological sources in South Sulawesi and to evaluate the antagonistic activity of selected isolates against Fusarium sp. Sampling was conducted from May to July 2025 in a national park, forestry plant nursery, silkworm rearing centers, natural forests, plantation forests, community forests, and agroforestry systems. Insects, soil, and plant tissues were used as fungal isolation sources. Fungal isolates were characterized based on their morphological features and classified as entomopathogenic (EP), entomopathogenic endophytic (EEF), or endophytic (END) fungi according to their respective sources and observed characteristics. Three selected isolates were evaluated for antagonistic activity against Fusarium sp. using a dual-culture assay, and the data were analyzed using one-way analysis of variance (ANOVA) followed by Tukey’s HSD test. A total of 72 fungal isolates representing nine fungal taxa were recovered from the different sampling sources. The isolates included Aspergillus sp., Simplicillium subtropicum, Penicillium sp., Fusarium sp., Cladosporium oxysporum, Trichoderma sp., Cordyceps sp., Gibellula sp., and the microsporidian pathogen Nosema bombycis. Among the selected isolates, S. subtropicum sp. KuBi1, B. bassiana, BGRSP, and C. oxysporum KeTi1 significantly inhibited the radial growth of Fusarium sp. compared with the untreated control (P < 0.05), with the highest percentage inhibition of radial growth reaching 56% for KeTi1. These findings indicate that the selected entomopathogenic fungi have potential as biological control candidates against Fusarium sp.; however, further greenhouse and field studies are required to validate their efficacy and assess their suitability for development as microbial biopesticides.

Keywords

Collaboration network of the authors

40 authors · 118 co-authorship links · 26 across different organisations · showing the 40 most connected

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • Hasanuddin University
  • Universitas Halu Oleo
  • East–West Center
  • Diponegoro University
  • Universitas Gadjah Mada
  • Institut Agama Islam Negeri Kendari
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Andi Detti YuniantiHasanuddin University3
Astuti ArifHasanuddin University2
SuhasmanAffiliation unknown2
Rinaldi SjahrilAffiliation unknown2
Feranita HaringAffiliation unknown2
BaharuddinAffiliation unknown2
Nurul HasfiDiponegoro University1
Ahsan YunusHasanuddin University1
Muhammad Alif K. SahideHasanuddin University1
Micah R. FisherEast–West Center1
Emban Ibnurusyd Mas’udAffiliation unknown1
Fatwa FaturachmatHasanuddin University1
Ahmad MaryudiUniversitas Gadjah Mada1
Hamirul HadiniUniversitas Halu Oleo1
MIRZA ARSIATY ARSYADUniversitas Halu Oleo1
Waode Siti Anima HiseinUniversitas Halu Oleo1
Dirvamena BoerUniversitas Halu Oleo1
NAWIR NAWIRUniversitas Halu Oleo1
SUAIB SUAIBUniversitas Halu Oleo1
Edwin NlAffiliation unknown1
AgussalimAffiliation unknown1
Ira TaskirawatiHasanuddin University1
Asmi Citra MalinaHasanuddin University1
Ahmad AhmadInstitut Agama Islam Negeri Kendari1
Muhammad Fuad AnshoriHasanuddin University1
Muh. Farid BdrHasanuddin University1
Yunus MusaHasanuddin University1
ACHMAD KAUTSAR BAHARUDDINHasanuddin University1
Rostyati HasyimAffiliation unknown1
Muhammad SadarAffiliation unknown1
Abdul FattahAffiliation unknown1
Beta PutrantoHasanuddin University1
SyahidahAffiliation unknown1
Nurul Muhlisah MompewaHasanuddin University1
G GusmiatyHasanuddin University1
Kurnia KurniaHasanuddin University1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) http://jhpttropika.fp.unila.ac.id/index.php/jhpttropika/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en">Screening of entomopathogenic and endophytic fungi from various landscapes and evaluation of their antagonistic activity against Fusarium sp.</dc:title>
	<dc:creator xml:lang="en">Nuraeni, Sitti</dc:creator>
	<dc:creator xml:lang="en"> Larekeng, Siti Halimah</dc:creator>
	<dc:creator xml:lang="en">Mario, M. Bayu</dc:creator>
	<dc:creator xml:lang="en">Prastiyo, Andi</dc:creator>
	<dc:subject xml:lang="en">Antagonistic activity</dc:subject>
	<dc:subject xml:lang="en">Beauveria sp.</dc:subject>
	<dc:subject xml:lang="en">entomopathogenic endophytic fungi</dc:subject>
	<dc:subject xml:lang="en">Fusarium sp.</dc:subject>
	<dc:subject xml:lang="en">C. oxysporum</dc:subject>
	<dc:subject xml:lang="en">S.        subtropicum</dc:subject>
	<dc:description xml:lang="en">Forestry research is increasingly focused on exploring microbial resources, including entomopathogenic endophytic fungi (EEFs), as potential biological control agent. This study aimed to explore and characterize fungal isolates obtained from different landscapes and biological sources in South Sulawesi and to evaluate the antagonistic activity of selected isolates against Fusarium sp. Sampling was conducted from May to July 2025 in a national park, forestry plant nursery, silkworm rearing centers, natural forests, plantation forests, community forests, and agroforestry systems. Insects, soil, and plant tissues were used as fungal isolation sources. Fungal isolates were characterized based on their morphological features and classified as entomopathogenic (EP), entomopathogenic endophytic (EEF), or endophytic (END) fungi according to their respective sources and observed characteristics. Three selected isolates were evaluated for antagonistic activity against Fusarium sp. using a dual-culture assay, and the data were analyzed using one-way analysis of variance (ANOVA) followed by Tukey’s HSD test. A total of 72 fungal isolates representing nine fungal taxa were recovered from the different sampling sources. The isolates included Aspergillus sp., Simplicillium subtropicum, Penicillium sp., Fusarium sp., Cladosporium oxysporum, Trichoderma sp., Cordyceps sp., Gibellula sp., and the microsporidian pathogen Nosema bombycis. Among the selected isolates, S. subtropicum sp. KuBi1, B. bassiana, BGRSP, and C. oxysporum KeTi1 significantly inhibited the radial growth of Fusarium sp. compared with the untreated control (P &amp;lt; 0.05), with the highest percentage inhibition of radial growth reaching 56% for KeTi1. These findings indicate that the selected entomopathogenic fungi have potential as biological control candidates against Fusarium sp.; however, further greenhouse and field studies are required to validate their efficacy and assess their suitability for development as microbial biopesticides.</dc:description>
	<dc:publisher xml:lang="en">Universitas Lampung</dc:publisher>
	<dc:date>2026-09-17</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
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	<dc:identifier>https://jhpttropika.fp.unila.ac.id/index.php/jhpttropika/article/view/1049</dc:identifier>
	<dc:identifier>10.23960/j.hptt.226572-588</dc:identifier>
	<dc:source xml:lang="en">Jurnal Hama dan Penyakit Tumbuhan Tropika; Vol. 26 No. 2 (2026): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PESTS AND DISEASES; 572-588</dc:source>
	<dc:source>2461-0399</dc:source>
	<dc:source>1411-7525</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://jhpttropika.fp.unila.ac.id/index.php/jhpttropika/article/view/1049/731</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2026 Jurnal Hama dan Penyakit Tumbuhan Tropika</dc:rights>
	<dc:rights xml:lang="en">https://creativecommons.org/licenses/by-nc/4.0</dc:rights>
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