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In Vitro Growth and Development of Progenies Derived from Crossings of Several Dendrobium Accessions Under Different Media

AGRIVITA, Journal of Agricultural Science · Vol. 47 No. 2 (2025) · pp. 368–381

DOI
10.17503/agrivita.v47i2.4505
ISSN
2477-8516, 0126-0537
Published
2025-06-03
Publisher
Faculty of Agriculture University of Brawijaya in collaboration with PERAGI
Language
eng
License
creativecommons.org/licenses/by-nc/4.0

Abstract

The objective of plant hybridization is to generate alternative superior phenotypes with the aim of decreasing the dependence on wild orchid exploitation in nature. Although interspecies crosses in Dendrobium are very likely to occur and there is a low chance of experiencing bud depression, interspecies crosses have the potential to encounter several problems, including failure of embryo development resulted to the seeds unable to germinate. Media formulation is the most essential component in the success of in vitro culture of orchid seeds. Therefore, this study aimed to obtain new Dendrobium hybrids and the best media formulation for germination and seedling growth. A total of three experiments were conducted including hybridization, the impacts of basic media namely (1) MS, (2) VW, and (3) foliar fertilizer both with and without the addition of tryptone, on seedling germination. The results showed that there was no compatibility barriers observed in all crosses. Foliar fertilizer + tryptone media was recommended for orchid seedlings because it produced better growth and roots up to 8 months after planting (MAP). For best results on Dendrobium hybrid seedlings, MS + tryptone media up to 6 MAP was used followed by deflasking into VW + tryptone to stimulate optimal root growth before hardening.

Keywords

Collaboration network of the authors

40 authors · 94 co-authorship links · 1 across different organisations · showing the 40 most connected

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • Universitas Halu Oleo
  • State University of Jakarta
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Adi Noor PrayogiAffiliation unknown4
Paul B TimotiwuAffiliation unknown3
Joko JokoUniversitas Halu Oleo2
Dulbari DulbariAffiliation unknown2
Ni Siluh Putu NuryantiAffiliation unknown2
Ekawati DanialAffiliation unknown2
Wiry PungkastianiAffiliation unknown2
Setyo Dwi UtomoAffiliation unknown2
Hayane Adeline WarganegaraAffiliation unknown2
Nanik SriyaniAffiliation unknown2
La Ode Herman HalikaAffiliation unknown1
Novi NoviAffiliation unknown1
La IbaUniversitas Halu Oleo1
Fitriani FitrianiAffiliation unknown1
Adrian TawaiUniversitas Halu Oleo1
Silviatun NihayahAffiliation unknown1
Wening SariAffiliation unknown1
Intan RazariAffiliation unknown1
Kinasih PrayuniAffiliation unknown1
Ahmad Rusdan Handoyo UtomoAffiliation unknown1
Yovitha YuliejantiningsihAffiliation unknown1
Dini RakhmawatiAffiliation unknown1
Nabila LutfiahAffiliation unknown1
erlinda citra dewiAffiliation unknown1
Devi MaharaniAffiliation unknown1
M Syamsoel HadiAffiliation unknown1
Galuh M PramudyaAffiliation unknown1
Sudarsono SudarsonoAffiliation unknown1
Widodo WidodoState University of Jakarta1
. YUSNITAAffiliation unknown1
Willy Bayuardi SuwarnoAffiliation unknown1
Sintho Wahyuning ArdieAffiliation unknown1
Uswatun ChusnahAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://agrivita.ub.ac.id/index.php/agrivita/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en-US">In Vitro Growth and Development of Progenies Derived from Crossings of Several Dendrobium Accessions Under Different Media</dc:title>
	<dc:creator>Maulida, Desi</dc:creator>
	<dc:creator>Yusnita, Yusnita</dc:creator>
	<dc:creator>Hapsoro, Dwi</dc:creator>
	<dc:creator>Agustiansyah, Agustiansyah</dc:creator>
	<dc:creator>Karyanto, Agus</dc:creator>
	<dc:creator>Hamiranti, Rahmadyah</dc:creator>
	<dc:creator>Nugroho, Rizki Bagus</dc:creator>
	<dc:subject xml:lang="en-US">Agronomy, horticulture, and plant breeding</dc:subject>
	<dc:subject xml:lang="en-US">Dendrobium; Hybrid; Hybridization; Media formulation</dc:subject>
	<dc:description xml:lang="en-US">The objective of plant hybridization is to generate alternative superior phenotypes with the aim of decreasing the dependence on wild orchid exploitation in nature. Although interspecies crosses in Dendrobium are very likely to occur and there is a low chance of experiencing bud depression, interspecies crosses have the potential to encounter several problems, including failure of embryo development resulted to the seeds unable to germinate. Media formulation is the most essential component in the success of in vitro culture of orchid seeds. Therefore, this study aimed to obtain new Dendrobium hybrids and the best media formulation for germination and seedling growth. A total of three experiments were conducted including hybridization, the impacts of basic media namely (1) MS, (2) VW, and (3) foliar fertilizer both with and without the addition of tryptone, on seedling germination. The results showed that there was no compatibility barriers observed in all crosses. Foliar fertilizer + tryptone media was recommended for orchid seedlings because it produced better growth and roots up to 8 months after planting (MAP). For best results on Dendrobium hybrid seedlings, MS + tryptone media up to 6 MAP was used followed by deflasking into VW + tryptone to stimulate optimal root growth before hardening.</dc:description>
	<dc:publisher xml:lang="en-US">Faculty of Agriculture University of Brawijaya in collaboration with PERAGI</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2025-06-03</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://agrivita.ub.ac.id/index.php/agrivita/article/view/4505</dc:identifier>
	<dc:identifier>10.17503/agrivita.v47i2.4505</dc:identifier>
	<dc:source xml:lang="en-US">AGRIVITA Journal of Agricultural Science; Vol 47, No 2 (2025); 368-381</dc:source>
	<dc:source>2477-8516</dc:source>
	<dc:source>0126-0537</dc:source>
	<dc:source>10.17503/agrivita.v47i2</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/view/4505/1872</dc:relation>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/downloadSuppFile/4505/3404</dc:relation>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/downloadSuppFile/4505/3405</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2025 The Author(s)</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc/4.0</dc:rights>
</oai_dc:dc>