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Various pH Media Influence Production of Pseudomonas fluorescens P20 Raw Secondary Metabolites for Controlling Damping-off (Pythium sp.) in Cucumber Seedlings

AGRIVITA, Journal of Agricultural Science · Vol. 44 No. 1 (2022) · pp. 178–186

DOI
10.17503/agrivita.v44i1.2730
ISSN
2477-8516, 0126-0537
Published
2022-01-31
Publisher
Faculty of Agriculture University of Brawijaya in collaboration with PERAGI
Language
eng
License
creativecommons.org/licenses/by-nc/4.0

Abstract

This research aims to determine the best pH media for the production of Pseudomonas fluorescens P20 raw secondary metabolites, its effect on controlling damping-off, and on cucumber seedling growth. In vitro test uses completely randomized design with four replicates and seven treatments consisted of pH 5.0; 5.5; 6.0; 6.5; 7.0; 7.5; and 8.0. In planta test uses a randomized block design with three replicates and ten treatments consisting of control, mancozeb 80%, and raw secondary metabolites with pH 7.0 and 7.5, and 4 concentration levels, i.e., 5, 10, 15, and 20%. Variables observe population density, inhibition ability, protease and chitinase qualitatively, germination ability, incubation period, disease incidence, the area under disease progress curve (AUDPC), crop height, number of leaves, root length, and crop fresh weight. The result shows that the best pH for the production of raw secondary metabolites is 7.0, indicated by population density as 5.68 × 1024 cfu/ml, inhibition ability as 50.8%, and the best protease and chitinase qualitatively. Application of the secondary metabolites on pH 7.0 could suppress disease intensity, incubation period, and AUDPC as 66.67, 77%, and 0%-day, respectively, and increase crop height, the number of leaves, root length, and crop fresh weight as 57.65, 37.19, 63, and 74%, respectively.

Keywords

Collaboration network of the authors

40 authors · 96 co-authorship links · showing the 40 most connected

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  • Jenderal Soedirman University
  • Universitas Halu Oleo
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
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Co-authorOrganisationJoint articles
Ruth Feti RahayuniatiAffiliation unknown12
Murti Wisnu Ragil SastyawanAffiliation unknown11
Nur PrihatiningsihAffiliation unknown5
Ni Wayan Anik LeanaAffiliation unknown5
Ratna Stia DewiJenderal Soedirman University5
Kamarullah KamarullahAffiliation unknown4
Cut Intan SalasiyahAffiliation unknown4
Saprijal SaprijalAffiliation unknown4
La HamimuUniversitas Halu Oleo2
AMAR MA’RUFUniversitas Halu Oleo2
Etik Wukir TiniAffiliation unknown2
Husaini HusdaAffiliation unknown2
Rasyad RasyadAffiliation unknown2
Rahimah EmbongAffiliation unknown2
Muhammad Arif FadhilahAffiliation unknown2
Anwar DaudAffiliation unknown2
Luthfi AuniAffiliation unknown2
Sa'i Sa'iAffiliation unknown2
La Ode SiwiAffiliation unknown1
LA ODE SYAHRILAffiliation unknown1
Puji AnandaAffiliation unknown1
Muhammad FikarAffiliation unknown1
Ibnu MurbawanAffiliation unknown1
Damrin DamrinAffiliation unknown1
Kahirun KahirunUniversitas Halu Oleo1
Ruslin RuslinUniversitas Halu Oleo1
La Ode Muhammad ErifAffiliation unknown1
La Baco SudiaAffiliation unknown1
Surya Cipta Ramadhan KeteAffiliation unknown1
Roy AndreasAffiliation unknown1
Ernasiwi Astri OktaviliaAffiliation unknown1
Amelia Putri HeryawatiJenderal Soedirman University1
Reksa Satria AdijanaJenderal Soedirman University1
Difa Sri MuthiaJenderal Soedirman University1
Daffa Naufal FaiqJenderal Soedirman University1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://agrivita.ub.ac.id/index.php/agrivita/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en-US">Various pH Media Influence Production of Pseudomonas fluorescens P20 Raw Secondary Metabolites for Controlling Damping-off (Pythium sp.) in Cucumber Seedlings</dc:title>
	<dc:creator>Soesanto, Loekas</dc:creator>
	<dc:creator>Latifah, Siti</dc:creator>
	<dc:creator>Manan, Abdul</dc:creator>
	<dc:creator>Mugiastuti, Endang</dc:creator>
	<dc:creator>Suharti, Woro Sri</dc:creator>
	<dc:subject xml:lang="en-US"></dc:subject>
	<dc:subject xml:lang="en-US">Cucumber seedlings; Damping-off; pH media; Pseudomonas fluorescens; Raw secondary metabolites</dc:subject>
	<dc:description xml:lang="en-US"> This research aims to determine the best pH media for the production of Pseudomonas fluorescens P20 raw secondary metabolites, its effect on controlling damping-off, and on cucumber seedling growth. In vitro test uses completely randomized design with four replicates and seven treatments consisted of pH 5.0; 5.5; 6.0; 6.5; 7.0; 7.5; and 8.0. In planta test uses a randomized block design with three replicates and ten treatments consisting of control, mancozeb 80%, and raw secondary metabolites with pH 7.0 and 7.5, and 4 concentration levels, i.e., 5, 10, 15, and 20%. Variables observe population density, inhibition ability, protease and chitinase qualitatively, germination ability, incubation period, disease incidence, the area under disease progress curve (AUDPC), crop height, number of leaves, root length, and crop fresh weight. The result shows that the best pH for the production of raw secondary metabolites is 7.0, indicated by population density as 5.68 × 1024 cfu/ml, inhibition ability as 50.8%, and the best protease and chitinase qualitatively. Application of the secondary metabolites on pH 7.0 could suppress disease intensity, incubation period, and AUDPC as 66.67, 77%, and 0%-day, respectively, and increase crop height, the number of leaves, root length, and crop fresh weight as 57.65, 37.19, 63, and 74%, respectively.</dc:description>
	<dc:publisher xml:lang="en-US">Faculty of Agriculture University of Brawijaya in collaboration with PERAGI</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2022-01-31</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://agrivita.ub.ac.id/index.php/agrivita/article/view/2730</dc:identifier>
	<dc:identifier>10.17503/agrivita.v44i1.2730</dc:identifier>
	<dc:source xml:lang="en-US">AGRIVITA Journal of Agricultural Science; Vol 44, No 1 (2022); 178-186</dc:source>
	<dc:source>2477-8516</dc:source>
	<dc:source>0126-0537</dc:source>
	<dc:source>10.17503/agrivita.v44i1</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/view/2730/1470</dc:relation>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/downloadSuppFile/2730/1917</dc:relation>
	<dc:relation>https://agrivita.ub.ac.id/index.php/agrivita/article/downloadSuppFile/2730/1919</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2022 The Authors (s)</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc/4.0</dc:rights>
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