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Expression of Human Erythropoietin Containing 2 Additional N-Link in CHO-K1 Cells under Different Culture Conditions

Indonesian Journal of Cancer Chemoprevention · Vol. 10 No. 1 (2019) · pp. 7–15

DOI
10.14499/indonesianjcanchemoprev10iss1pp7-15
ISSN
2355-8989, 2088-0197
Published
2019-02-28
Publisher
Indonesian Society for Cancer Chemoprevention
Language
eng

Abstract

Human erythropoietin (hEPO) is a glycoprotein that regulates the formation of erythrocytes and mainly used in anemia patients. Previously, we have reported the expression of modified human EPO with 2 additional N-linked in mammalian cell CHO-K1. The aim of this current research was to study the optimum condition for modified recombinant hEPO (rhEPO) production in CHO-K1. To do this, several parameters of culture conditions were applied including antibiotic concentrations, seeding densities, time of incubations, fetal bovine serum (FBS) concentrations and cell culture media. The result showed that the presence of antibiotic G418 improved the expression level with the highest was at 1% of concentration. Meanwhile, seeding density of 2–3x105 cells/6 cm dish and seven day of incubation time were the best condition for rhEPO protein expression. From five different combination media used, F12 medium with 10% FBS gave the highest expression of rhEPO protein. From this study was also found that at passage 16 the expression level was still increasing proving that the clone expressing the protein of our interest is promisingly stable.Keywords: EPO, erythropoietin, protein expression, CHO-K1, optimation

Collaboration network of the authors

40 authors · 198 co-authorship links · showing the 40 most connected

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  • IPB University
  • Universitas Jember
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
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Co-authorOrganisationJoint articles
Pekik Wiji PrasetyaningrumAffiliation unknown14
Yana RubiyanaAffiliation unknown12
Ratna Dwi RamadaniAffiliation unknown9
Ratih Asmana NingrumAffiliation unknown8
Ahmad SuyokoAffiliation unknown6
Dian Fitria AgustiyantiAffiliation unknown4
Andri WardianaAffiliation unknown4
Khairul AnamUniversitas Jember4
Neny NurainiAffiliation unknown4
Edy MeiyantoAffiliation unknown3
Ria Fajarwati KastianAffiliation unknown3
Komang Alit ParamitasariAffiliation unknown3
Alayna Lillahida Indri HimawanAffiliation unknown3
Salsabila AzzahraAffiliation unknown3
Ferry IskandarAffiliation unknown2
Ernawati Arifin Giri-RachmanAffiliation unknown2
Hariyatun HariyatunAffiliation unknown2
Azzania FibrianiAffiliation unknown2
Fitri Aulia PermatasariAffiliation unknown2
Marissa AngelinaAffiliation unknown2
Ali DjunaediAffiliation unknown2
Sarjito SarjitoAffiliation unknown2
Ratna Asmah SusidartiAffiliation unknown2
Indri KusharyantiAffiliation unknown2
Wisnu Ananta KusumaIPB University1
Annisa AnnisaIPB University1
Fitri SuratinAffiliation unknown1
Obie FarobieAffiliation unknown1
Delicia Yunita RahmanAffiliation unknown1
Fendy FendyAffiliation unknown1
Nur Faiizah Aqiilah FirmanAffiliation unknown1
Naswandi NurAffiliation unknown1
Eri SahabudinAffiliation unknown1
Syaefudin SumintoAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://ijcc.chemoprev.org/index.php/ijcc/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en-US">Expression of Human Erythropoietin Containing 2 Additional N-Link in CHO-K1 Cells under Different Culture Conditions</dc:title>
	<dc:creator>Santoso, Adi</dc:creator>
	<dc:creator>Larasati, Larasati</dc:creator>
	<dc:creator>Kusumawati, Arizah</dc:creator>
	<dc:creator>Wisnuwardhani, Popi Hadi</dc:creator>
	<dc:creator>Ningrum, Ratih Asma</dc:creator>
	<dc:creator>Septisetyani, Endah Puji</dc:creator>
	<dc:description xml:lang="en-US">Human erythropoietin (hEPO) is a glycoprotein that regulates the formation of erythrocytes and mainly used in anemia patients. Previously, we have reported the expression of modified human EPO with 2 additional N-linked in mammalian cell CHO-K1. The aim of this current research was to study the optimum condition for modified recombinant hEPO (rhEPO) production in CHO-K1. To do this, several parameters of culture conditions were applied including antibiotic concentrations, seeding densities, time of incubations, fetal bovine serum (FBS) concentrations and cell culture media. The result showed that the presence of antibiotic G418 improved the expression level with the highest was at 1% of concentration. Meanwhile, seeding density of 2–3x105 cells/6 cm dish and seven day of incubation time were the best condition for rhEPO protein expression. From five different combination media used, F12 medium with 10% FBS gave the highest expression of rhEPO protein. From this study was also found that at passage 16 the expression level was still increasing proving that the clone expressing the protein of our interest is promisingly stable.Keywords : EPO, erythropoietin, protein expression, CHO-K1, optimation</dc:description>
	<dc:publisher xml:lang="en-US">Indonesian Society for Cancer Chemoprevention</dc:publisher>
	<dc:contributor xml:lang="en-US">Indonesian Institute of Sciences</dc:contributor>
	<dc:date>2019-02-28</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US"></dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://ijcc.chemoprev.org/index.php/ijcc/article/view/261</dc:identifier>
	<dc:identifier>10.14499/indonesianjcanchemoprev10iss1pp7-15</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Cancer Chemoprevention; Vol 10, No 1 (2019); 7-15</dc:source>
	<dc:source>2355-8989</dc:source>
	<dc:source>2088-0197</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://ijcc.chemoprev.org/index.php/ijcc/article/view/261/176</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2019 Adi Santoso</dc:rights>
</oai_dc:dc>