Melola Discovery

← Melola Discovery

Journal SINTA 1

The Cytotoxic Activity of Solanum Nigrum Ethanolic Extract on Widr Human Colon Cancer Cells

Indonesian Journal of Cancer Chemoprevention · Vol. 2 No. 3 (2011) · pp. 291–294

DOI
10.14499/indonesianjcanchemoprev2iss3pp291-294
ISSN
2355-8989, 2088-0197
Published
2011-10-31
Publisher
Indonesian Society for Cancer Chemoprevention
Language
eng

Abstract

Solanum nigrum L. or Leunca in Indonesia has been traditionally used as a herbal plant, which is believed to have anti-tumor properties, although the mechanism for the activity remains unknown. The resecarch aim to examine the cytotoxic effect of the ethanolic extract of Solanum nigrum on WiDr human colon cancer cells. In this study, we prepared an ethanol extract from herb of Solanum nigrum and investigated the mechanism involved in its growth-inhibitory effect on WiDr human colon cancer cells. Herbs of Solanum nigrum dry powder is extracted with 70% ethanol then added into the WiDr cell culture in 96 wells plate in various concentration: 50, 100, 250, and 500 µg/ml. Cytotoxicity of the Solanum nigrum ethanolic extract was analyzed with MTT assay on WiDr human colon cancer cell lines. Results from the MTT assay showed WiDr cells was weakly suppressed in the presence of the extract. The result of the assay also showed a very close correlation between the Solanum nigrum extract concentration and the surviving cell numbers which means the extract caused cell death in a dose-dependent fashion in WiDr cancer cells with the IC50 of 359,23 µg/ml. Collectively, the research suggest further studies to explore other chemopreventive possibilites of Solanum nigrum ethanolic extract.Keywords: colon cancer, MTT assay, cytotoxic, WiDr, Solanum nigrum

Collaboration network of the authors

40 authors · 147 co-authorship links · showing the 40 most connected

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • IPB University
  • Universitas Balikpapan
  • Universitas Halu Oleo
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Edy MeiyantoAffiliation unknown36
Rohmad Yudi UtomoAffiliation unknown6
Ratna Asmah SusidartiAffiliation unknown5
Riris Istighfari JenieAffiliation unknown5
Ummi Maryam ZulfinAffiliation unknown4
Erlina RivantiAffiliation unknown4
Sarmoko SarmokoAffiliation unknown3
Anindyajati AnindyajatiAffiliation unknown3
Yurista GilangAffiliation unknown3
Nanda Resa PratamaAffiliation unknown3
Aditya FitriasariAffiliation unknown3
Lailatul QodriaAffiliation unknown2
Ratih Kurnia WardaniAffiliation unknown2
Gergorius Gena MaranAffiliation unknown2
Nur IsmiyatiUniversitas Balikpapan2
Ratna Dwi RamadaniAffiliation unknown2
Nadzifa NugraheniAffiliation unknown2
Nurramadhani Armada SidaUniversitas Halu Oleo2
NormaidahAffiliation unknown2
Herwandhani PutriAffiliation unknown2
Larasati LarasatiIPB University2
Raditya Prima IstiajiAffiliation unknown2
Faaza Aulia RahmanAffiliation unknown2
Desty Restia RahmawatiAffiliation unknown2
Diyah Novi SekariniAffiliation unknown1
Yohanes Surya JatiAffiliation unknown1
Nur Ayunie ZulkepliAffiliation unknown1
Wulandari WulandariAffiliation unknown1
Dhania NovitasariAffiliation unknown1
Laeli MuntafiahAffiliation unknown1
Nur Fitra SariAffiliation unknown1
Nisa Ul HasanahAffiliation unknown1
Ahmad Syauqy TafrihaniAffiliation unknown1
Dhiya Ulhaq SalsabilaAffiliation unknown1
Ghina LintangsariAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://ijcc.chemoprev.org/index.php/ijcc/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">The Cytotoxic Activity of Solanum Nigrum Ethanolic Extract on Widr Human Colon Cancer Cells</dc:title>
	<dc:creator>Maruti, Astrid Ayu</dc:creator>
	<dc:creator>F., Ilham Augusta</dc:creator>
	<dc:creator>Putri, Dyaningtyas Dewi Pamungkas</dc:creator>
	<dc:creator>Hermawan, Adam</dc:creator>
	<dc:creator>Ikawati, Muthi'</dc:creator>
	<dc:description xml:lang="en-US">Solanum nigrum L. or Leunca in Indonesia has been traditionally used as a herbal plant, which is believed to have anti-tumor properties, although the mechanism for the activity remains unknown. The resecarch aim to examine the cytotoxic effect of the ethanolic extract of Solanum nigrum on WiDr human colon cancer cells. In this study, we prepared an ethanol extract from herb of Solanum nigrum and investigated the mechanism involved in its growth-inhibitory effect on WiDr human colon cancer cells. Herbs of Solanum nigrum dry powder is extracted with 70% ethanol then added into the WiDr cell culture in 96 wells plate in various concentration : 50, 100, 250, and 500 µg/ml. Cytotoxicity of the Solanum nigrum ethanolic extract  was analyzed with MTT assay on WiDr human colon cancer cell lines. Results from the MTT assay showed WiDr cells was weakly suppressed in the presence of the extract. The result of the assay also showed a very close correlation between the Solanum nigrum extract concentration and the surviving cell numbers which means the extract caused cell death in a dose-dependent fashion in WiDr cancer cells with the IC50 of 359,23 µg/ml. Collectively, the research suggest further studies to explore other chemopreventive possibilites of Solanum nigrum ethanolic extract.Keywords : colon cancer, MTT assay, cytotoxic, WiDr, Solanum nigrum </dc:description>
	<dc:publisher xml:lang="en-US">Indonesian Society for Cancer Chemoprevention</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2011-10-31</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US"></dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://ijcc.chemoprev.org/index.php/ijcc/article/view/45</dc:identifier>
	<dc:identifier>10.14499/indonesianjcanchemoprev2iss3pp291-294</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Cancer Chemoprevention; Vol 2, No 3 (2011); 291-294</dc:source>
	<dc:source>2355-8989</dc:source>
	<dc:source>2088-0197</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://ijcc.chemoprev.org/index.php/ijcc/article/view/45/46</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2017 Indonesian Journal of Cancer Chemoprevention</dc:rights>
</oai_dc:dc>