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Secang (Caesalpinia sappan L.) Heartwood Ethanolic Extract Shows Activity as Doxorubicin Co-chemotherapeutic Agent by Apoptotis Induction on T47D Breast Cancer Cells

Indonesian Journal of Cancer Chemoprevention · Vol. 3 No. 2 (2012) · pp. 376–383

DOI
10.14499/indonesianjcanchemoprev3iss2pp376-383
ISSN
2355-8989, 2088-0197
Published
2012-06-30
Publisher
Indonesian Society for Cancer Chemoprevention
Language
eng

Abstract

Doxorubicin, primary chemoteurapeutic agent used for breast cancer treatment, is known to have various side effects included multi drug resistance (MDR) phenomenon. Therefore, exploration of co-chemotherapeutic agent is important to be conducted in order to prevent MDR. Secang (Caesalpinia sappan L.) which contains active compounds brazilin and brazilein, is proven to have activity as anticancer. The aim of this study is to determine the potency of Caesalpinia sappan L. ethanolic extract (CEE) as co-chemotherapeutic agent of doxorubicin and its mechanism through apoptosis induction on T47D breast cancer cells. Caesalpinia sappan L. heartwood powder was macerated with ethanol 70%. The cytotoxic effect of CEE alone and its combination with doxorubicin was analyzed using MTT assay. Apoptosis assay was done by flowcytometry-annexin V method. CEE showed cytotoxic activity on T47D cells with IC50 value of 35 µg/ml, while combinatorial test showed that all of combination doses of CEE and doxorubicin gave synergistic effect. Flowcytometry-annexin V assay proved that treatment of CEE induced apoptosis of doxorubicin. Based on these results, we conclude that Caesalpinia sappan L. heartwood ethanolic extract is potential to be developed as co-chemotherapeutic agent of doxorubicin.Keywords: Caesalpinia sappan L., doxorubicin, apoptosis, T47D cells

Collaboration network of the authors

40 authors · 138 co-authorship links · showing the 40 most connected

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  • University of Brawijaya
  • IPB University
  • Universitas Balikpapan
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Adam HermawanAffiliation unknown19
Ratna Asmah SusidartiAffiliation unknown17
Muthi’ IkawatiAffiliation unknown15
Riris Istighfari JenieAffiliation unknown14
Rohmad Yudi UtomoAffiliation unknown9
Sari HaryantiAffiliation unknown5
Faaza Aulia RahmanAffiliation unknown5
Anindyajati AnindyajatiAffiliation unknown5
Dhania NovitasariAffiliation unknown4
Retno MurwantiAffiliation unknown4
Desty Restia RahmawatiAffiliation unknown4
Ummi Maryam ZulfinAffiliation unknown4
Raditya Prima IstiajiAffiliation unknown4
Herwandhani PutriAffiliation unknown4
Sri HandayaniUniversity of Brawijaya3
Larasati LarasatiIPB University3
Sarmoko SarmokoAffiliation unknown3
Yonika Arum LarasatiAffiliation unknown3
Nanda Resa PratamaAffiliation unknown3
Masashi KawaichiAffiliation unknown2
Sendy JunediAffiliation unknown2
Nunuk Aries NurulitaAffiliation unknown2
Suwijiyo PramonoAffiliation unknown2
Zalinar UdinAffiliation unknown2
Yogi ErtantoAffiliation unknown2
Lailatul QodriaAffiliation unknown2
Gergorius Gena MaranAffiliation unknown2
Aulia Nur SeptianiAffiliation unknown2
Sofa FaridaAffiliation unknown2
Beni LestariAffiliation unknown2
Asri Mega PutriAffiliation unknown2
Hilyatul FadliyahAffiliation unknown2
Indri KusharyantiAffiliation unknown2
Ni Putu Linda LaksmianiAffiliation unknown2
Nur IsmiyatiUniversitas Balikpapan2
Astrid Ayu MarutiAffiliation unknown2

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://ijcc.chemoprev.org/index.php/ijcc/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en-US">Secang (Caesalpinia sappan L.) Heartwood Ethanolic Extract Shows Activity as Doxorubicin Co-chemotherapeutic Agent by Apoptotis Induction on T47D Breast Cancer Cells</dc:title>
	<dc:creator>Nurzijah, Ika</dc:creator>
	<dc:creator>Putri, Dyaningtyas Dewi Pamungkas</dc:creator>
	<dc:creator>Rivanti, Erlina</dc:creator>
	<dc:creator>Meiyanto, Edy</dc:creator>
	<dc:description xml:lang="en-US">Doxorubicin, primary chemoteurapeutic agent used for breast cancer treatment, is known to have various side effects included multi drug resistance (MDR) phenomenon. Therefore, exploration of co-chemotherapeutic agent is important to be conducted in order to prevent MDR. Secang (Caesalpinia sappan L.) which contains active compounds brazilin and brazilein, is proven to have activity as anticancer. The aim of this study is to determine the potency of Caesalpinia sappan L. ethanolic extract (CEE) as co-chemotherapeutic agent of doxorubicin and its mechanism through apoptosis induction on T47D breast cancer cells. Caesalpinia sappan L. heartwood powder was macerated with ethanol 70%. The cytotoxic effect of CEE alone and its combination with doxorubicin was analyzed using MTT assay. Apoptosis assay was done by flowcytometry-annexin V method. CEE showed cytotoxic activity on T47D cells with IC50 value of 35 µg/ml, while combinatorial test showed that all of combination doses of CEE and doxorubicin gave synergistic effect. Flowcytometry-annexin V assay proved that treatment of CEE induced apoptosis of doxorubicin. Based on these results, we conclude that Caesalpinia sappan L. heartwood ethanolic extract is potential to be developed as co-chemotherapeutic agent of doxorubicin.Keywords : Caesalpinia sappan L., doxorubicin, apoptosis, T47D cells</dc:description>
	<dc:publisher xml:lang="en-US">Indonesian Society for Cancer Chemoprevention</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2012-06-30</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US"></dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://ijcc.chemoprev.org/index.php/ijcc/article/view/59</dc:identifier>
	<dc:identifier>10.14499/indonesianjcanchemoprev3iss2pp376-383</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Cancer Chemoprevention; Vol 3, No 2 (2012); 376-383</dc:source>
	<dc:source>2355-8989</dc:source>
	<dc:source>2088-0197</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://ijcc.chemoprev.org/index.php/ijcc/article/view/59/60</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2017 Indonesian Journal of Cancer Chemoprevention</dc:rights>
</oai_dc:dc>