Spermatozoa Quality of Bali Cattle Following Sexing using Egg Yolk Tris Medium at Different Duration

Kesumanengsih, Viny Aprilia; Aka, Rahim; Saili, Takdir

doi:10.33772/jitro.v9i2.22020

Open access Journal SINTA 2

Spermatozoa Quality of Bali Cattle Following Sexing using Egg Yolk Tris Medium at Different Duration

JITRO (Jurnal Ilmu dan Teknologi Peternakan Tropis) · Vol. 9 No. 2 (2022) · pp. 375–380

DOI: 10.33772/jitro.v9i2.22020
ISSN: 2406-9337
Published: 2022-05-31
Publisher: Universitas Halu Oleo
Language: eng
License: creativecommons.org/licenses/by-sa/4.0

Abstract

The length of sexing duration may affect the quality of Bali cattle spermatozoa. This study aims to examine the effect of sexing duration on viability, motility and abnormalities of Bali cattle spermatozoa. This research has been carried out at Regional Technical Implementation Unit (RTIU) Center for Breeding and Animal Feed, Department of Food Crops and Livestock, Southeast Sulawesi Province. The material used was a bull of Bali cattle as a source of semen and spermatozoa to be evaluated. In this study, a completely randomized design (CRD) was used with 3 treatments and 6 replications. The treatments consisted of three different length of sexing duration namely 40 min. (P1), 50 min. (P2), and 60 min. (P3). The variables evaluated in this study included semen quality (volume, color, odor, consistency and pH) and spermatozoa (mass movement, concentration, percentage of motility, viability and abnormalities). The data obtained were analyzed using analysis of variance (ANOVA) and continued with the Least Significant Difference test (LSD test). The results showed that the average volume of Bali cattle semen obtained was 5,38 ml/ejaculate, semen color was milky white, odor was specific, consistency was viscous, pH was 6,3, spermatozoa concentration was 1,445 million/ml and mass movement was +++. Whereas the motility of spermatozoa in each treatment following sexing were 76,67% (P1), 68,23% (P2), and 60,00% (P3). The viability of spermatozoa after sexing were 93,42% (P1), 84,42% (P2), and 79,83% (P3). The abnormality of spermatozoa after sexing were 2,58% (P1), 2,67% (P2), and 2,67% (P3). Furthermore, the results of variance analysis showed that the length of sexing duration had a significant effect (P˂0.05) on the viability and motility of the Bali cattle spermatozoa, but did not significantly affect the sperm abnormalities of Bali cattle. The 40 min. (treatment P1) and 50 min. (treatment P2) of sexing duration resulted in better spermatozoa viability and motility of Bali cattle compared to 60 min. (treatment P3) of sexing duration.Keywords: abnormality, motility, sexing, spermatozoa, viability

Collaboration network of the authors

40 authors · 230 co-authorship links · showing the 40 most connected

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Universitas Halu Oleo
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Co-author	Organisation	Joint articles
Natsir Sandiah	Universitas Halu Oleo	7
La Ode Nafiu	Universitas Halu Oleo	7
Nur Santy Asminaya	Universitas Halu Oleo	7
Harapin Hafid	Universitas Halu Oleo	6
Andi Murlina Tasse	Universitas Halu Oleo	6
Rusli Badaruddin	Universitas Halu Oleo	6
Deki Zulkarnain	Universitas Halu Oleo	6
Muhammad Amrullah Pagala	Universitas Halu Oleo	6
Takdir Saili	Universitas Halu Oleo	5
Achmad Selamet Aku	Universitas Halu Oleo	5
Nuraini Nuraini	Universitas Halu Oleo	4
La Ode Arsad Sani	Universitas Halu Oleo	4
Muh. Rusdin	Universitas Halu Oleo	4
La Ode Ba’a	Universitas Halu Oleo	3
Fuji Astuty Auza	Universitas Halu Oleo	3
La Malesi	Universitas Halu Oleo	3
Asma Bio Kimestri	Universitas Halu Oleo	3
Dian Agustina	Affiliation unknown	3
Hairil Azulyatno Hadini	Universitas Halu Oleo	2
Salomina Sambolinggi	Universitas Halu Oleo	2
Rahman Rahman	Universitas Halu Oleo	2
Fitrianingsih Fitrianingsih	Universitas Halu Oleo	2
Rachmita Dewi S. Toba	Universitas Halu Oleo	2
Widhi Kurniawan	Universitas Halu Oleo	2
Hamdan Has	Universitas Halu Oleo	2
Mohamad Agus Setiadi	Affiliation unknown	2
Srihadi Agungpriyono	Affiliation unknown	2
Arief Boediono	Affiliation unknown	2
La Ode Satriawan	Affiliation unknown	1
Wa Ode Hasran	Affiliation unknown	1
Farida Asana	Universitas Halu Oleo	1
. Syamsuddin	Affiliation unknown	1
Suardin Suardin	Universitas Halu Oleo	1
Sitti Isyqzamiyah	Universitas Halu Oleo	1
Fifi Hariani	Universitas Halu Oleo	1
Sri Jumiati	Universitas Halu Oleo	1
Arni Arni	Universitas Halu Oleo	1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://ojs.uho.ac.id/index.php/peternakan-tropis/oai, first harvested 29 Oct 2026, last seen 8 Nov 2026.

Original metadata (oai_dc)

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	<dc:title xml:lang="en-US">Spermatozoa Quality of Bali Cattle Following Sexing using Egg Yolk Tris Medium at Different Duration</dc:title>
	<dc:creator>Kesumanengsih, Viny Aprilia</dc:creator>
	<dc:creator>Aka, Rahim</dc:creator>
	<dc:creator>Saili, Takdir</dc:creator>
	<dc:description xml:lang="en-US">The length of sexing duration may affect the quality of Bali cattle spermatozoa. This study aims to examine the effect of sexing duration on viability, motility and abnormalities of Bali cattle spermatozoa. This research has been carried out at Regional Technical Implementation Unit (RTIU) Center for Breeding and Animal Feed, Department of Food Crops and Livestock, Southeast Sulawesi Province. The material used was a bull of Bali cattle as a source of semen and spermatozoa to be evaluated. In this study, a completely randomized design (CRD) was used with 3 treatments and 6 replications. The treatments consisted of three different length of sexing duration namely 40 min. (P1), 50 min. (P2), and 60 min. (P3). The variables evaluated in this study included semen quality (volume, color, odor, consistency and pH) and spermatozoa (mass movement, concentration, percentage of motility, viability and abnormalities). The data obtained were analyzed using analysis of variance (ANOVA) and continued with the Least Significant Difference test (LSD test). The results showed that the average volume of Bali cattle semen obtained was 5,38 ml/ejaculate, semen color was milky white, odor was specific, consistency was viscous, pH was 6,3, spermatozoa concentration was 1,445 million/ml and mass movement was +++. Whereas the motility of spermatozoa in each treatment following sexing were 76,67% (P1), 68,23% (P2), and 60,00% (P3).  The viability of spermatozoa after sexing were 93,42% (P1), 84,42% (P2), and 79,83% (P3). The abnormality of spermatozoa after sexing were 2,58% (P1), 2,67% (P2), and 2,67% (P3).  Furthermore, the results of variance analysis showed that the length of sexing duration had a significant effect (P˂0.05) on the viability and motility of the Bali cattle spermatozoa, but did not significantly affect the sperm abnormalities of Bali cattle. The 40 min. (treatment P1) and 50 min. (treatment P2) of sexing duration resulted in better spermatozoa viability and motility of Bali cattle compared to 60 min. (treatment P3) of sexing duration.Keywords:  abnormality, motility, sexing, spermatozoa, viability</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Halu Oleo</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2022-05-31</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://ojs.uho.ac.id/index.php/peternakan-tropis/article/view/22020</dc:identifier>
	<dc:identifier>10.33772/jitro.v9i2.22020</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Ilmu dan Teknologi Peternakan Tropis; Vol 9, No 2 (2022): JITRO, May 2022; 375-380</dc:source>
	<dc:source>2406-9337</dc:source>
	<dc:source>10.33772/jitro.v9i2</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://ojs.uho.ac.id/index.php/peternakan-tropis/article/view/22020/pdf</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2022 JITRO (Jurnal Ilmu dan Teknologi Peternakan Tropis)</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
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