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Induction of Matrix Metalloproteinases in Chondrocytes by Interleukin IL-1β as an Osteoarthritis Model

Journal of Mathematical and Fundamental Sciences · Vol. 51 No. 2 (2019) · pp. 103–111

DOI
10.5614/j.math.fund.sci.2019.51.2.1
ISSN
2338-5510, 2337-5760
Published
2019-08-02
Publisher
Directorate for Research and Innovation (DRI) ITB
Language
eng

Abstract

Osteoarthritis (OA) is a chronic disease of the joints and bones due to trauma or other joint-related diseases (secondary). Synovial inflammation commonly causes disturbance in joint homeostasis, which is associated with OA. Enzymes such as aggrecanase and metalloproteinase generate cartilage damage, mediated by tumor necrosis factor (TNF-α) and interleukin (IL)-1. Pro-inflammatory cytokines, including TNF-α, IL-1β, and IL-6, are responsible for regulation of the extracellular matrix, cartilage degradation, and apoptosis of chondrocytes. This study aimed to observe the cell viability and expression level of matrix metalloproteinases (MMP-1 and MMP-3) and tissue inhibitor metalloproteinases (TIMP-1 and TIMP-2) in human chondrocyte cells (CHON-002) induced by IL-1β. CHON-002 was induced with IL-1β (0.1, 1 and 10 ng/mL) as an OA model. The viability of the cells was measured with a 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxyme-thoxyphenyl)-2-(4-sulfophenyl)-2H-tetra zolium (MTS) assay, while expression of MMP-1, MMP-3, TIMP-1, and TIMP-2, was evaluated by RT-PCR. The viability of IL-1β-induced CHON-002 (CHON-002- IL-1β) cells at day 1 and 5 showed that treatment with up to 10 ng/mL of IL-1β was not toxic. Expression of TIMP-1 and TIMP-2 in CHON-002-IL-1β was lower compared to control, while that of MMP-1 and MMP-3 was higher compared to control. These results indicate that CHON-002 treated with 10 ng/mL IL-1β has expression patterns consistent with chondrocyte damage, so the CHON-002-IL-1β system may serve as a model for MMP induction in OA.

Keywords

Collaboration network of the authors

40 authors · 206 co-authorship links · 1 across different organisations · showing the 40 most connected

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  • Universitas Halu Oleo
  • Indonesia University of Education
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
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Co-authorOrganisationJoint articles
Hanna Sari Widya KusumaAffiliation unknown16
Ermi GirsangAffiliation unknown8
Diana Krisanti JasaputraAffiliation unknown8
Philips OnggowidjajaAffiliation unknown8
I Nyoman Ehrich ListerAffiliation unknown7
Didik PriyandokoIndonesia University of Education7
Chrismis Novalinda GintingAffiliation unknown6
Dhanar Septyawan HadiprasetyoAffiliation unknown5
HANA RATNAWATIAffiliation unknown5
Lusiana DarsonoAffiliation unknown5
Chandra RisdianAffiliation unknown5
Wahyu Setia WidodoAffiliation unknown5
Rizal AzisAffiliation unknown4
Teresa Liliana WargasetiaAffiliation unknown4
Arief BoedionoAffiliation unknown4
AHMAD FARIEDAffiliation unknown4
Ika Adhani SholihahAffiliation unknown4
Dian Ratih LaksmitawatiAffiliation unknown4
Marisca Evalina GondokesumoAffiliation unknown3
Gian PrimahanaAffiliation unknown3
Sofa FajriahAffiliation unknown3
Ricson Pemimpin HutagaolAffiliation unknown3
Kelvin YapriantoAffiliation unknown3
Mawar SubangkitAffiliation unknown3
The Fransiska EltaniaAffiliation unknown2
Agung NoviantoAffiliation unknown2
Muhammad Eka PrastyaAffiliation unknown2
Fadhilah Haifa ZahirohAffiliation unknown2
Mohamad GazaliAffiliation unknown2

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journals.itb.ac.id/index.php/jmfs/oai, first harvested 9 Nov 2026, last seen 9 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en-US">Induction of Matrix Metalloproteinases in Chondrocytes by Interleukin IL-1β as an Osteoarthritis Model</dc:title>
	<dc:creator>Afifah, Ervi</dc:creator>
	<dc:creator>Mozef, Tjandrawati</dc:creator>
	<dc:creator>Sandra, Ferry</dc:creator>
	<dc:creator>Arumwardana, Seila</dc:creator>
	<dc:creator>Rihibiha, Dwi Davidson</dc:creator>
	<dc:creator>Nufus, Hayatun</dc:creator>
	<dc:creator>Rizal, Rizal</dc:creator>
	<dc:creator>Amalia, Annisa</dc:creator>
	<dc:creator>Bachtiar, Indra</dc:creator>
	<dc:creator>Murti, Harry</dc:creator>
	<dc:creator>Widowati, Wahyu</dc:creator>
	<dc:subject xml:lang="en-US">interleukin</dc:subject>
	<dc:subject xml:lang="en-US">mesenchymal stem cell</dc:subject>
	<dc:subject xml:lang="en-US">metalloproteases</dc:subject>
	<dc:subject xml:lang="en-US">MMP genes</dc:subject>
	<dc:subject xml:lang="en-US">osteoarthritis</dc:subject>
	<dc:description xml:lang="en-US">Osteoarthritis (OA) is a chronic disease of the joints and bones due to trauma or other joint-related diseases (secondary). Synovial inflammation commonly causes disturbance in joint homeostasis, which is associated with OA. Enzymes such as aggrecanase and metalloproteinase generate cartilage damage, mediated by tumor necrosis factor (TNF-α) and interleukin (IL)-1. Pro-inflammatory cytokines, including TNF-α, IL-1β, and IL-6, are responsible for regulation of the extracellular matrix, cartilage degradation, and apoptosis of chondrocytes. This study aimed to observe the cell viability and expression level of matrix metalloproteinases (MMP-1 and MMP-3) and tissue inhibitor metalloproteinases (TIMP-1 and TIMP-2) in human chondrocyte cells (CHON-002) induced by IL-1β. CHON-002 was induced with IL-1β (0.1, 1 and 10 ng/mL) as an OA model. The viability of the cells was measured with a 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxyme-thoxyphenyl)-2-(4-sulfophenyl)-2H-tetra zolium (MTS) assay, while expression of MMP-1, MMP-3, TIMP-1, and TIMP-2, was evaluated by RT-PCR. The viability of IL-1β-induced CHON-002 (CHON-002- IL-1β) cells at day 1 and 5 showed that treatment with up to 10 ng/mL of IL-1β was not toxic. Expression of TIMP-1 and TIMP-2 in CHON-002-IL-1β was lower compared to control, while that of MMP-1 and MMP-3 was higher compared to control. These results indicate that CHON-002 treated with 10 ng/mL IL-1β has expression patterns consistent with chondrocyte damage, so the CHON-002-IL-1β system may serve as a model for MMP induction in OA.</dc:description>
	<dc:publisher xml:lang="en-US">Directorate for Research and Innovation (DRI) ITB</dc:publisher>
	<dc:date>2019-08-02</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journals.itb.ac.id/index.php/jmfs/article/view/8428</dc:identifier>
	<dc:identifier>10.5614/j.math.fund.sci.2019.51.2.1</dc:identifier>
	<dc:source xml:lang="en-US">Journal of Mathematical and Fundamental Sciences; Vol. 51 No. 2 (2019); 103-111</dc:source>
	<dc:source>2338-5510</dc:source>
	<dc:source>2337-5760</dc:source>
	<dc:source>10.5614/j.math.fund.sci.2019.51.2</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journals.itb.ac.id/index.php/jmfs/article/view/8428/3837</dc:relation>
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