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Piper crocatum Ameliorates APAP-Induced Hepatotoxicity through Antioxidant and Anti-Inflammatory Mechanisms

Journal of Mathematical and Fundamental Sciences · Vol. 54 No. 1 (2022) · pp. 109–120

DOI
10.5614/j.math.fund.sci.2022.54.1.6
ISSN
2338-5510, 2337-5760
Published
2022-06-21
Publisher
Directorate for Research and Innovation (DRI) ITB
Language
eng

Abstract

Cirrhosis is a serious hepatic disease that occurs worldwide and is caused by progressive fibrosis in the liver. Free radicals are the major cause of reactive oxygen species (ROS) generation, which affects the balance of the liver metabolism. Piper crocatum, commonly called red betel, is widely used in ethnomedicine because it has biological capabilities, including antioxidant and anti-inflammatory properties. The present study was undertaken to examine the possibility of hepaprotective activity of red betel extract on acetaminophen (APAP)-induced HepG2 cells as the cirrhosis in vitro model. Assessment of red betel extract (RBE) was performed using the colorimetric method for the quantification of LDH, AST, and ALT. As preliminary study, a cytotoxicity assay was performed at various RBE concentrations (100.00; 50.00; 25.00; 12.50; 6.25; 3.13 μg/ml) using an MTS assay to decide the safe concentration based on cell viability. The RBE treatment did not affect cell viability, even at a high concentration (100 µg/ml, p > 0.05). RBE at concentrations 25 and 100 µg/ml successfully reduced LDH, AST, and ALT activities in the hepatotoxic model in a dose-dependent manner. In the APAP-induced hepatotoxicity model, RBE positively indicated a hepatoprotective effect and cell amelioration by a decrease in hepatotoxic markers.

Keywords

Collaboration network of the authors

40 authors · 206 co-authorship links · 1 across different organisations · showing the 40 most connected

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  • Universitas Halu Oleo
  • Indonesia University of Education
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Hanna Sari Widya KusumaAffiliation unknown10
Ervi AfifahAffiliation unknown10
Annisa AmaliaAffiliation unknown10
Teresa Liliana WargasetiaAffiliation unknown7
Dhanar Septyawan HadiprasetyoAffiliation unknown6
Tjandrawati MozefAffiliation unknown6
Didik PriyandokoIndonesia University of Education6
AHMAD FARIEDAffiliation unknown6
Diana Krisanti JasaputraAffiliation unknown6
Ali Napiah NasutionAffiliation unknown6
The Fransiska EltaniaAffiliation unknown5
Rizal AzisAffiliation unknown4
Marisca Evalina GondokesumoAffiliation unknown4
Edy FachrialAffiliation unknown4
Seila ArumwardanaAffiliation unknown4
Indra BachtiarAffiliation unknown4
Dwi Davidson RihibihaAffiliation unknown4
Harry MurtiAffiliation unknown4
HANA RATNAWATIAffiliation unknown3
Lusiana DarsonoAffiliation unknown3
Ika Adhani SholihahAffiliation unknown3
Philips OnggowidjajaAffiliation unknown3
Fadhilah Haifa ZahirohAffiliation unknown3
Ferry SandraAffiliation unknown3
Tan SuyonoAffiliation unknown2
Agung NoviantoAffiliation unknown2
Chandra RisdianAffiliation unknown2
Munason MunasonAffiliation unknown1
Muhammad IlhamAffiliation unknown1
Hedi HediAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journals.itb.ac.id/index.php/jmfs/oai, first harvested 9 Nov 2026, last seen 9 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en-US">Piper crocatum Ameliorates APAP-Induced Hepatotoxicity through Antioxidant and Anti-Inflammatory Mechanisms</dc:title>
	<dc:creator>Lister, I Nyoman Ehrich</dc:creator>
	<dc:creator>Ginting, Chrismis Novalinda</dc:creator>
	<dc:creator>Girsang, Ermi</dc:creator>
	<dc:creator>Amansyah, Adek</dc:creator>
	<dc:creator>Chiuman, Linda</dc:creator>
	<dc:creator>Yanti, Ni Luh Wisma Eka</dc:creator>
	<dc:creator>Widodo, Wahyu Setia</dc:creator>
	<dc:creator>Yusepany, Dewani Tediana</dc:creator>
	<dc:creator>Rizal, Rizal</dc:creator>
	<dc:creator>Widowati, Wahyu</dc:creator>
	<dc:subject xml:lang="en-US">aminotransferase</dc:subject>
	<dc:subject xml:lang="en-US">alanine aminotransferase</dc:subject>
	<dc:subject xml:lang="en-US">cirrhosis</dc:subject>
	<dc:subject xml:lang="en-US">lactate dehydrogenase</dc:subject>
	<dc:subject xml:lang="en-US">red betel</dc:subject>
	<dc:description xml:lang="en-US">Cirrhosis is a serious hepatic disease that occurs worldwide and is caused by progressive fibrosis in the liver. Free radicals are the major cause of reactive oxygen species (ROS) generation, which affects the balance of the liver metabolism. Piper crocatum, commonly called red betel, is widely used in ethnomedicine because it has biological capabilities, including antioxidant and anti-inflammatory properties. The present study was undertaken to examine the possibility of hepaprotective activity of red betel extract on acetaminophen (APAP)-induced HepG2 cells as the cirrhosis in vitro model. Assessment of red betel extract (RBE) was performed using the colorimetric method for the quantification of LDH, AST, and ALT. As preliminary study, a cytotoxicity  assay was performed at various RBE concentrations (100.00; 50.00; 25.00; 12.50; 6.25; 3.13 μg/ml) using an MTS assay to decide the safe concentration based on cell viability. The RBE treatment did not affect cell viability, even at a high concentration (100 µg/ml, p &amp;gt; 0.05). RBE at concentrations 25 and 100 µg/ml successfully reduced LDH, AST, and ALT activities in the hepatotoxic model in a dose-dependent manner. In the APAP-induced hepatotoxicity model, RBE positively indicated a hepatoprotective effect and cell amelioration by a decrease in hepatotoxic markers.</dc:description>
	<dc:publisher xml:lang="en-US">Directorate for Research and Innovation (DRI) ITB</dc:publisher>
	<dc:date>2022-06-21</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journals.itb.ac.id/index.php/jmfs/article/view/13499</dc:identifier>
	<dc:identifier>10.5614/j.math.fund.sci.2022.54.1.6</dc:identifier>
	<dc:source xml:lang="en-US">Journal of Mathematical and Fundamental Sciences; Vol. 54 No. 1 (2022); 109-120</dc:source>
	<dc:source>2338-5510</dc:source>
	<dc:source>2337-5760</dc:source>
	<dc:source>10.5614/j.math.fund.sci.2022.54.1</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journals.itb.ac.id/index.php/jmfs/article/view/13499/5846</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2022 Journal of Mathematical and Fundamental Sciences</dc:rights>
</oai_dc:dc>