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Peptone and tomato extract induced early stage of embryo development of Dendrobium phalaenopsis Orchid

Journal of Tropical Biodiversity and Biotechnology · Vol. 1 No. 2 (2017) · pp. 77–84

DOI
10.22146/jtbb.15498
ISSN
2540-9581, 2540-9573
Published
2017-04-11
Publisher
Universitas Gadjah Mada
Language
eng
License
creativecommons.org/licenses/by-sa/4.0

Abstract

Germination and growth of orchid seeds can be accelerated by the addition of organic supplement and plant extract in culture medium. The objective of this study was to determine the effect of peptone and tomato extract on early stage of embryo development of Dendrobium phalaenopsis orchids. Orchid seeds were sown on NP and VW medium with addition of 10% of CW (NPCW and VWCW). Five weeks after seed germination, about 58.03% seed germination was observed on VWCW medium, and only 37.45% seed germination on NPCW. Tomato extract and peptone were added in VWCW, resulting VWCWTP medium. After 4-8 weeks on VWCWTP, 94.42% seeds was germinated into plantlet, but only 67.30% germinated seeds on VWCW. To get optimal growth and development of D. phalaenopsis orchids embryos in the in vitro condition, supplement of 100 ml.L-1 coconut water, 100 mg.L-1 tomato extract and 2 mg.L-1 peptone into VW basic medium is required.

Collaboration network of the authors

40 authors · 100 co-authorship links · showing the 40 most connected

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  • Universitas Gadjah Mada
  • Affiliation unknown
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  • Same organisation
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Show as table
Co-authorOrganisationJoint articles
Ari IndriantoAffiliation unknown7
Muhammad Dylan LawrieAffiliation unknown7
Exsyupransia MursyantiAffiliation unknown6
Agus Budi SetiawanAffiliation unknown5
Windi MoseAffiliation unknown4
Yulita NurchayatiAffiliation unknown3
Febri Yuda KurniawanAffiliation unknown3
Jose Gutierrez-MarcosAffiliation unknown3
Jaka WidadaUniversitas Gadjah Mada3
Aries Bagus SasongkoUniversitas Gadjah Mada3
Endang SulistyaningsihAffiliation unknown2
Dyah Weny RespatieAffiliation unknown2
Chee How TeoAffiliation unknown2
Aviesta LinggabuwanaAffiliation unknown2
Saifa Usni PutriAffiliation unknown2
Nuzlan RasjidAffiliation unknown2
Sismindari SismindariAffiliation unknown2
Fitriana PuspitasariAffiliation unknown2
Naufal Ghozi Aditya PerdanaAffiliation unknown2
Dika SundariAffiliation unknown2
Triono Bagus SaputroAffiliation unknown2
Erma PrihastantiAffiliation unknown1
Maria UlvaAffiliation unknown1
Khoirin NidaAffiliation unknown1
Masrukhatul LuaeliyahAffiliation unknown1
Munifatul IzzatiAffiliation unknown1
Asadi AsadiAffiliation unknown1
Sahiral YakubAffiliation unknown1
Ari WibowoAffiliation unknown1
Maria Marina HerawatiAffiliation unknown1
Endang PudjihartatiAffiliation unknown1
Suwijiyo PramonoAffiliation unknown1
Tri Joko RaharjoAffiliation unknown1
Stalis Norma EthicaAffiliation unknown1
Oedjijono OedjijonoAffiliation unknown1
Irna AuliauzzakiaAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/jtbb/oai, first harvested 9 Nov 2026, last seen 9 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en-US">Peptone and tomato extract induced early stage of embryo development of Dendrobium  phalaenopsis Orchid</dc:title>
	<dc:creator>Setiari, Nintya</dc:creator>
	<dc:creator>Purwantoro, Aziz</dc:creator>
	<dc:creator>Moeljopawiro, Sukarti</dc:creator>
	<dc:creator>Semiarti, Endang</dc:creator>
	<dc:subject xml:lang="en-US"></dc:subject>
	<dc:description xml:lang="en-US">Germination and growth of orchid seeds can be accelerated by the addition of organic supplement and plant extract in culture medium. The objective of this study was to determine the effect of peptone and tomato extract on early stage of embryo development of Dendrobium phalaenopsis orchids. Orchid seeds were sown on NP and VW medium with addition of 10% of CW (NPCW and VWCW).  Five weeks after seed germination, about 58.03% seed germination was observed on VWCW medium, and only 37.45% seed germination on NPCW. Tomato extract and peptone were added in VWCW, resulting VWCWTP medium. After 4-8 weeks on VWCWTP, 94.42% seeds was germinated into plantlet, but only 67.30% germinated seeds on VWCW. To get optimal growth and development of  D.  phalaenopsis orchids embryos in the in vitro condition, supplement of 100 ml.L-1 coconut water, 100 mg.L-1 tomato extract and 2 mg.L-1 peptone into VW basic medium is required.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US">WUR Project 2015 Universitas Gadjah Mada Indonesia</dc:contributor>
	<dc:date>2017-04-11</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US"></dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/jtbb/article/view/15498</dc:identifier>
	<dc:identifier>10.22146/jtbb.15498</dc:identifier>
	<dc:source xml:lang="en-US">Journal of Tropical Biodiversity and Biotechnology; Vol 1, No 2 (2016): December; 77-84</dc:source>
	<dc:source>2540-9581</dc:source>
	<dc:source>2540-9573</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/view/15498/16377</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2017 Journal of Tropical Biodiversity and Biotechnology</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>