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RAPD Analysis for Genetic Variability Detection of Mutant Soybean (Glycine max (L.) Merr)

Journal of Tropical Biodiversity and Biotechnology · Vol. 5 No. 1 (2020) · pp. 68–77

DOI
10.22146/jtbb.53653
ISSN
2540-9581, 2540-9573
Published
2020-04-14
Publisher
Universitas Gadjah Mada
Language
eng
License
creativecommons.org/licenses/by-sa/4.0

Abstract

This study aimed to detect and evaluate the genetic mutation from mutagenized soybean by RAPD markers. Soybean seeds of “Grobogan” variety were treated with two different concentrations of EMS (0.5% and 1%) and three incubation times (4, 6 and 8 h). DNA whole-genome was isolated from young leaf seedling with the Qiagen DNeasy Plant Mini Kit. Twenty OPA primers (OPA-1 to OPA-20) were used for DNA amplification. The results showed that EMS treatments successfully generated genetic variation in soybean, which indicated by high values of PIC, EMR, and MI. RAPD primers that effective to detect the mutation were OPA-2, OPA-07, OPA-10, OPA-11, OPA-12, OPA-13, OPA-14, OPA-15, OPA-16, OPA-18 and OPA-20. The band expression of those primers was exhibited a stronger intensity along with increasing of EMS concentration and incubation time used in this study. Treatment of 0.5% EMS in 6 hours incubation was successfully generated soybean mutants with the lowest genetic similarity compared to the wild-type. Thus, this study provides a new approach to generate genetic variability in soybean and has the potential to improve for soybean breeding program.

Keywords

Collaboration network of the authors

38 authors · 142 co-authorship links · 10 across different organisations

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • Indonesian Institute of Sciences
  • National Research and Innovation Agency
  • University of Brawijaya
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Shofiyatul Mas’udahIndonesian Institute of Sciences3
Dewi Ayu LestariIndonesian Institute of Sciences3
Setyawan Agung DanartoIndonesian Institute of Sciences2
Janis DamaiyaniIndonesian Institute of Sciences2
Trimanto TrimantoAffiliation unknown2
Siti NurfadilahAffiliation unknown2
Suyono SuyonoAffiliation unknown2
Ruri Siti ResmisariAffiliation unknown2
Elok Rifqi FirdianaNational Research and Innovation Agency2
Rasyadan Taufiq ProbojatiAffiliation unknown2
TrimantoAffiliation unknown1
Sugeng BudihartaIndonesian Institute of Sciences1
TITUT YULISTYARINIIndonesian Institute of Sciences1
Rachmawan Adi LaksonoIndonesian Institute of Sciences1
Nurlaily LaviantiAffiliation unknown1
Bekti YunantoAffiliation unknown1
Fauziah FauziahAffiliation unknown1
Bagas PratesoAffiliation unknown1
Eriyanto YusnawanAffiliation unknown1
Ridesti RindyastutiAffiliation unknown1
Ilham Kurnia AbywijayaAffiliation unknown1
Ruth Feti RahayuniatiAffiliation unknown1
Siti SubandiyahAffiliation unknown1
Gebby Agnessya Esa OktaviaAffiliation unknown1
Kurniawan BudiartoAffiliation unknown1
Shofiyatul MasudahAffiliation unknown1
Ahmad Syahrian SiregarAffiliation unknown1
Syahran WaelAffiliation unknown1
Elga RenjanaIndonesian Institute of Sciences1
Linda Wige NingrumNational Research and Innovation Agency1
Apriyono RahadiantoroAffiliation unknown1
Fitriyah FitriyahAffiliation unknown1
Imey Tamara IndiviaAffiliation unknown1
Rodiyati AzrianingsihUniversity of Brawijaya1
Isna Arofatun NikmahAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/jtbb/oai, first harvested 9 Nov 2026, last seen 9 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en-US">RAPD Analysis for Genetic Variability Detection of Mutant Soybean (Glycine max (L.) Merr)</dc:title>
	<dc:creator>Wahyudi, Didik</dc:creator>
	<dc:creator>Hapsari, Lia</dc:creator>
	<dc:creator>Sundari, S.</dc:creator>
	<dc:subject xml:lang="en-US">ethyl methane sulphonate; genetic mutation; RAPD; soybean</dc:subject>
	<dc:description xml:lang="en-US">This study aimed to detect and evaluate the genetic mutation from mutagenized soybean by RAPD markers. Soybean seeds of “Grobogan” variety were treated with two different concentrations of EMS (0.5% and 1%) and three incubation times (4, 6 and 8 h). DNA whole-genome was isolated from young leaf seedling with the Qiagen DNeasy Plant Mini Kit. Twenty OPA primers (OPA-1 to OPA-20) were used for DNA amplification. The results showed that EMS treatments successfully generated genetic variation in soybean, which indicated by high values of PIC, EMR, and MI. RAPD primers that effective to detect the mutation were OPA-2, OPA-07, OPA-10, OPA-11, OPA-12, OPA-13, OPA-14, OPA-15, OPA-16, OPA-18 and OPA-20. The band expression of those primers was exhibited a stronger intensity along with increasing of EMS concentration and incubation time used in this study. Treatment of 0.5% EMS in 6 hours incubation was successfully generated soybean mutants with the lowest genetic similarity compared to the wild-type. Thus, this study provides a new approach to generate genetic variability in soybean and has the potential to improve for soybean breeding program.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2020-04-14</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
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	<dc:identifier>https://journal.ugm.ac.id/jtbb/article/view/53653</dc:identifier>
	<dc:identifier>10.22146/jtbb.53653</dc:identifier>
	<dc:source xml:lang="en-US">Journal of Tropical Biodiversity and Biotechnology; Vol 5, No 1 (2020): April; 68-77</dc:source>
	<dc:source>2540-9581</dc:source>
	<dc:source>2540-9573</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/view/53653/27415</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/downloadSuppFile/53653/10907</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/downloadSuppFile/53653/10908</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2020 Journal of Tropical Biodiversity and Biotechnology</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
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