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Effect of Carbon Source Variations on Growth, Physiological Stress, and Saponin Levels of Talinum paniculatum Gaertn. Adventitious Roots

Journal of Tropical Biodiversity and Biotechnology · Vol. 7 No. 3 (2022)

DOI
10.22146/jtbb.69359
ISSN
2540-9581, 2540-9573
Published
2022-09-23
Publisher
Universitas Gadjah Mada
Language
eng
License
creativecommons.org/licenses/by-sa/4.0

Abstract

Monosaccharide and disaccharide as carbon sources can affect the production of secondary metabolites. The study aims to determine the effect of variations in carbon sources on growth, physiological stress, and saponin levels of the adventitious roots of Talinum paniculatum Gaerthn. Adventitious roots are subculture in liquid MS medium treated with various sugars: 3% sucrose, 3% glucose, 3% fructose, 3% lactose, 3% maltose, 3% dextrose, sucrose + fructose (1.5% + 1.5%), sucrose + glucose (1.5% + 1.5%), glucose + fructose (1.5% + 1.5%), sucrose + dextrose (1.5% + 1.5%) for 6 weeks. The results of this study show that the 3% fructose treatment produces the highest fresh and dry biomass, which are 1.30 g and 0.23 g compared to the control. The morphology of adventitious roots in the treatment of carbon source variation is not different from the control treatment. The highest MDA (malondialdehyde) levels are found in the sucrose + fructose treatment (1.5% + 1.5%). Meanwhile, the highest proline levels are found in the 3% maltose treatment. Saponin levels analyzed using thin layer chromatography show the data in the form of color intensity and stain area based on ImageJ software analysis. The 3% fructose treatment shows the highest color intensity and stain area compared to the control. Variations in carbon sources affect physiological stress, biomass, and saponin levels of adventitious roots of T. paniculatum, but do not effect on root morphology.

Keywords

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23 authors · 73 co-authorship links

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Anjar Tri WibowoAffiliation unknown4
Dannis Yuda KusumaAffiliation unknown4
Boon Chin TanAffiliation unknown3
Sugiharto -Affiliation unknown2
Dwi WinarniAffiliation unknown2
Ufairanisa IslamatasyaAffiliation unknown2
Gebby Agnessya Esa OktaviaAffiliation unknown1
Junairiah JunairiahAffiliation unknown1
Palupi Dasawulan LestariAffiliation unknown1
Syifa FajrisaniAffiliation unknown1
Putri GehastiAffiliation unknown1
Nur Afni JayantiAffiliation unknown1
Angelia Krisdanti Nasera PutriAffiliation unknown1
Nabilatun Nisa’Affiliation unknown1
Idqa Nurtri BhaktiAffiliation unknown1
Sugiharto SugihartoAffiliation unknown1
Abdul Hakim MuhsyiAffiliation unknown1
Ahimsa Buena MerpatiAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/jtbb/oai, first harvested 9 Nov 2026, last seen 9 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en-US">Effect of Carbon Source Variations on Growth, Physiological Stress, and Saponin Levels of Talinum paniculatum Gaertn. Adventitious Roots</dc:title>
	<dc:creator>Erin, Nindi Novia</dc:creator>
	<dc:creator>Yachya, Arif</dc:creator>
	<dc:creator>Kristanti, Alfinda Novi</dc:creator>
	<dc:creator>Sugiarso, Djarot</dc:creator>
	<dc:creator>Manuhara, Yosephine Sri Wulan</dc:creator>
	<dc:subject xml:lang="en-US">biology</dc:subject>
	<dc:subject xml:lang="en-US">adventitious root; carbon source; proline; saponin; Talinum paniculatum</dc:subject>
	<dc:description xml:lang="en-US">Monosaccharide and disaccharide as carbon sources can affect the production of secondary metabolites. The study aims to determine the effect of variations in carbon sources on growth, physiological stress, and saponin levels of the adventitious roots of Talinum paniculatum Gaerthn. Adventitious roots are subculture in liquid MS medium treated with various sugars: 3% sucrose, 3% glucose, 3% fructose, 3% lactose, 3% maltose, 3% dextrose, sucrose + fructose (1.5% + 1.5%), sucrose + glucose (1.5% + 1.5%), glucose + fructose (1.5% + 1.5%), sucrose + dextrose (1.5% + 1.5%) for 6 weeks. The results of this study show that the 3% fructose treatment produces the highest fresh and dry biomass, which are 1.30 g and 0.23 g compared to the control. The morphology of adventitious roots in the treatment of carbon source variation is not different from the control treatment. The highest MDA (malondialdehyde) levels are found in the sucrose + fructose treatment (1.5% + 1.5%). Meanwhile, the highest proline levels are found in the 3% maltose treatment. Saponin levels analyzed using thin layer chromatography show the data in the form of color intensity and stain area based on ImageJ software analysis. The 3% fructose treatment shows the highest color intensity and stain area compared to the control. Variations in carbon sources affect physiological stress, biomass, and saponin levels of adventitious roots of T. paniculatum, but do not effect on root morphology.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US">Airlangga University via Mandatory Research Grant, No. 1408/UN3/2019.</dc:contributor>
	<dc:date>2022-09-23</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
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	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/jtbb/article/view/69359</dc:identifier>
	<dc:identifier>10.22146/jtbb.69359</dc:identifier>
	<dc:source xml:lang="en-US">Journal of Tropical Biodiversity and Biotechnology; Vol 7, No 3 (2022): December; jtbb69359</dc:source>
	<dc:source>2540-9581</dc:source>
	<dc:source>2540-9573</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/view/69359/34452</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/downloadSuppFile/69359/18512</dc:relation>
	<dc:relation>https://journal.ugm.ac.id/jtbb/article/downloadSuppFile/69359/18513</dc:relation>
	<dc:coverage xml:lang="en-US">South East Asia</dc:coverage>
	<dc:coverage xml:lang="en-US"></dc:coverage>
	<dc:coverage xml:lang="en-US"></dc:coverage>
	<dc:rights xml:lang="en-US">Copyright (c) 2022 Journal of Tropical Biodiversity and Biotechnology</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>