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Group A β-hemolytic Streptococcus Detection Using Anti-Outer Membrane Protein (OMP) Immunoglobulin G (IgG)

Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences · Vol. 7 No. 1 (2017) · pp. 8–15

DOI
10.11594/jtls.07.01.02
ISSN
2527-4376, 2087-5517
Published
2017-01-31
Publisher
Journal of Tropical Life Science
Language
eng

Abstract

Streptococcal pharyngitis sequel such as Rheumatic Fever (RF) or Rheumatic Heart Disease (RHD) is an autoimmune response mediated by T cells and IgG. Since it is an autoimmune process, the result of bacterial culture as the gold standard of diagnosis often shows negative results. IgG against the 33 kDa OMP is considered as an important mediator in the process of these autoimmune diseases, so its presence in blood serum can be used as a diagnostic tool. The purpose of this study is to prove that 33 kDa OMP is one of the immunogenic parts of the Streptococcus Group A β-hemolytic, so it is expected that IgG anti-33 kDa OMP can recognize and respond the bacteria and to support the probability of the Streptococcus Group A β-hemolytic infection. This study was a laboratory experimental study with a control group design. Animal used was RattusNovergicus immunized with whole cell bacteria or 33 kDa OMP mixed with Complete Freund’s Adjuvant or Incomplete Freund’s Adjuvant. Polyclonal IgG was obtained by drawing blood serum from the animals after immunization with Streptococcus Group A β-hemolytic for 4 weeks (A; n = 5) and 8 weeks (B; n = 5) or immunization with OMP 33 kDa for 4 weeks (C; n = 5) and 8 weeks (D; n = 5) and also negative control group (E; n = 5). Immunological tests were done using Dot Blot assay, ELISA, and immunocytochemical examination. The data obtained was then evaluated with statistical tests Kruskal-Wallis, Mann-Whitney and Repeated ANOVA (p 0.05). Dot Blot and immunocytochemical tests indicated that IgG anti-33 kDa OMP were able to recognize and respond the Streptococcus Group A β-hemolytic antigen. This study concluded that 33 kDa OMP was the immunogenic part of the bacteria and that IgG anti-33 kDa OMP could recognize and respond the Streptococcus Group A β-hemolytic bacteria.Â

Keywords

Collaboration network of the authors

38 authors · 94 co-authorship links

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  • University of Brawijaya
  • State University of Semarang
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
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Co-authorOrganisationJoint articles
Sigit PriatmokoState University of Semarang1
Catur HerisonAffiliation unknown1
Rustikawati RustikawatiAffiliation unknown1
Merakati HandayaningsihAffiliation unknown1
IS HELIANTIAffiliation unknown1
Agustin Krisna WardaniAffiliation unknown1
Tri Yudani Mardining RarasAffiliation unknown1
Mauludy Jutta AjrullahAffiliation unknown1
Ellen Fenix GunawanAffiliation unknown1
Nadya Shafa PramestiAffiliation unknown1
Maretha HamidaAffiliation unknown1
Elfi Anis SaatiAffiliation unknown1
Brian Fo’era-era DaelyAffiliation unknown1
Susanto NugrohoAffiliation unknown1
Rinaldi LengganaAffiliation unknown1
Aulanni’am Aulanni’amAffiliation unknown1
Tinny Endang HernowatiAffiliation unknown1
Avin Ainur FitrianingsihAffiliation unknown1
Lailia Nur RachmaAffiliation unknown1
Alvi MillianaAffiliation unknown1
Sanarto SantosoAffiliation unknown1
Umi KalsumAffiliation unknown1
Bambang RahardjoAffiliation unknown1
Siti Candra Windu BaktiyaniAffiliation unknown1
Sultanah ZahariahAffiliation unknown1
Tatit NursetaAffiliation unknown1
Riny NatalinaAffiliation unknown1
Erni YudaningtyasUniversity of Brawijaya1
Edi WidjajantoAffiliation unknown1
Mas MansyurAffiliation unknown1
Nashi WidodoUniversity of Brawijaya1
Oktavia Rahayu AdianingsihAffiliation unknown1
Valentina YurinaAffiliation unknown1
Suryanata KesumaAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) http://jtrolis.ub.ac.id/index.php/jtrolis/oai, first harvested 9 Nov 2026, last seen 9 Nov 2026.
Original metadata (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Group A β-hemolytic Streptococcus Detection Using  Anti-Outer Membrane Protein (OMP) Immunoglobulin G (IgG)</dc:title>
	<dc:creator xml:lang="en">Alluza, Hamid Hunaif Dhofi</dc:creator>
	<dc:creator xml:lang="en">Mayasari, Ema Dianita</dc:creator>
	<dc:creator xml:lang="en">Prawiro, Sumarno Reto</dc:creator>
	<dc:creator xml:lang="en">Winarsih, Sri</dc:creator>
	<dc:subject xml:lang="en">Streptococcus Group A β-hemolytic</dc:subject>
	<dc:subject xml:lang="en">33 kDa OMP</dc:subject>
	<dc:subject xml:lang="en">IgG anti-33 kDa OMP</dc:subject>
	<dc:description xml:lang="en">Streptococcal pharyngitis sequel such as Rheumatic Fever (RF) or Rheumatic Heart Disease (RHD) is an autoimmune response mediated by T cells and IgG. Since it is an autoimmune process, the result of bacterial culture as the gold standard of diagnosis often shows negative results. IgG against the 33 kDa OMP is considered as an important mediator in the process of these autoimmune diseases, so its presence in blood serum can be used as a diagnostic tool. The purpose of this study is to prove that 33 kDa OMP is one of the immunogenic parts of the Streptococcus Group A β-hemolytic, so it is expected that IgG anti-33 kDa OMP can recognize and respond the bacteria and to support the probability of the Streptococcus Group A β-hemolytic infection. This study was a laboratory experimental study with a control group design. Animal used was RattusNovergicus immunized with whole cell bacteria or 33 kDa OMP mixed with Complete Freund’s Adjuvant or Incomplete Freund’s Adjuvant. Polyclonal IgG was obtained by drawing blood serum from the animals after immunization with Streptococcus Group A β-hemolytic for 4 weeks (A; n = 5) and 8 weeks (B; n = 5) or immunization with OMP 33 kDa for 4 weeks (C; n = 5) and 8 weeks (D; n = 5) and also negative control group (E; n = 5). Immunological tests were done using Dot Blot assay, ELISA, and immunocytochemical examination. The data obtained was then evaluated with statistical tests Kruskal-Wallis, Mann-Whitney and Repeated ANOVA (p &amp;lt; 0.05). The result showed that there was a difference in humoral immune response (IgG) between the groups albeit the difference was not significant (p &amp;gt; 0.05). Dot Blot and immunocytochemical tests indicated that IgG anti-33 kDa OMP were able to recognize and respond the Streptococcus Group A β-hemolytic antigen. This study concluded that 33 kDa OMP was the immunogenic part of the bacteria and that IgG anti-33 kDa OMP could recognize and respond the Streptococcus Group A β-hemolytic bacteria. </dc:description>
	<dc:publisher xml:lang="en">Journal of Tropical Life Science</dc:publisher>
	<dc:date>2017-01-31</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/522</dc:identifier>
	<dc:identifier>10.11594/jtls.07.01.02</dc:identifier>
	<dc:source xml:lang="en">Journal of Tropical Life Science; Vol. 7 No. 1 (2017); 8-15</dc:source>
	<dc:source>2527-4376</dc:source>
	<dc:source>2087-5517</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/522/pdf</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2017 Journal of Tropical Life Science</dc:rights>
</oai_dc:dc>