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Predominant Bacterial Diversity in Rheumatoid Arthritis Rat After Treated with Caprine CSN1S2 Protein

Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences · Vol. 11 No. 2 (2021)

DOI
10.11594/jtls.11.02.01
ISSN
2527-4376, 2087-5517
Published
2021-06-01
Publisher
Journal of Tropical Life Science
Language
eng

Abstract

Introduction: Rheumatoid arthritis (RA) is an autoimmune and systemic inflammatory disease influenced by microbial abnormalities (dysbiosis) in the intestinal that affect changes in metabolism and immune system disorders. This study aimed to investigate the predominant intestinal microbiota in complete Freund’s Adjuvant-induced rheumatoid arthritis rats after treated with caprine milk CSN1S2 protein through fecal analysis based on PCR-DGGE and to construct the phylogenetic tree of bacteria as the evolutionary relationship. Method: The experimental animals were divided into 6 groups with 2 types of rat model, namely control rat (C group, CM group, and CY group) and RA rat (RA group, RAM group, and RAY group). Predominant cultivable microbiota was obtained by direct culture and analyzed using PCR-DGGE with some specific primers. The DNA sequences were analyzed and aligned using bioinformatics software to construct the phylogenetic tree. Results: We found that Lactobacillus group significantly increased in the control rat model and the predominant intestinal bacteria in RA rats were Enterococcus group (Enterococcus faecium and Enterococcus faecalis). Conclusions: The caprine milk CSN1S2 protein influences the composition of microbiota in RA rats with the emergence of predominant bacteria that are considered species of the Bacillus group, closely related to Bacillus coagulans, which can be promoted the growth of B. coagulans to suppress pathogenic bacteria in the development of RA disease.

Keywords

Collaboration network of the authors

40 authors · 95 co-authorship links · 1 across different organisations · showing the 40 most connected

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  • Lampung University
  • Universitas Ivet
  • Institut Teknologi Sepuluh Nopember
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Agus SuyatnaLampung University2
Syafruddin IlyasAffiliation unknown1
Fauzi YusufAffiliation unknown1
Harun Al Rasyid DamanikAffiliation unknown1
Sri Rahayu LestariAffiliation unknown1
Muhammad Sasmito DjatiAffiliation unknown1
Ahmad RudijantoAffiliation unknown1
Wayan SuanaLampung University1
Witri Puspita SariAffiliation unknown1
Meta Dwi AyuningtiasAffiliation unknown1
Legborsi NwinehAffiliation unknown1
Hervin MaulinaAffiliation unknown1
Karina WijayaAffiliation unknown1
Doni AndraAffiliation unknown1
Afis PratamaUniversitas Ivet1
Annisya DestryatiAffiliation unknown1
I Wayan DistrikLampung University1
Chandra ErtikantoLampung University1
Novinta NurulsariAffiliation unknown1
Abdurrahman AbdurrahmanAffiliation unknown1
Bambang SetiawanInstitut Teknologi Sepuluh Nopember1
Suharjono SuharjonoAffiliation unknown1
Takeshi OhtaAffiliation unknown1
Vita AgustinaAffiliation unknown1
Titin Andri WihastutiAffiliation unknown1
Yoga Tribakti RachmadAffiliation unknown1
Katsuhiro MiyajimaAffiliation unknown1
wahyu nur laili fajriAffiliation unknown1
Ahmad Rofi'iAffiliation unknown1
Istiftakhun NikmahAffiliation unknown1
Mulyati MulyatiAffiliation unknown1
Rista Nikmatu RohmahAffiliation unknown1
Damai Aulia NurmasariAffiliation unknown1
Nuri MasruroAffiliation unknown1
Lidwina Faraline TriprisilaAffiliation unknown1
Takahisa YamadaAffiliation unknown1
Takashi OhtaAffiliation unknown1
Dewi Ratih Tirto SariAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) http://jtrolis.ub.ac.id/index.php/jtrolis/oai, first harvested 9 Nov 2026, last seen 9 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en">Predominant Bacterial Diversity in Rheumatoid Arthritis Rat After Treated with Caprine CSN1S2 Protein</dc:title>
	<dc:creator xml:lang="en">Suyanto, Eko</dc:creator>
	<dc:creator xml:lang="en">Fatchiyah, Fatchiyah</dc:creator>
	<dc:subject xml:lang="en">Dysbiosis</dc:subject>
	<dc:subject xml:lang="en">Caprine</dc:subject>
	<dc:subject xml:lang="en">CSN1S2</dc:subject>
	<dc:subject xml:lang="en">Enterococcus</dc:subject>
	<dc:subject xml:lang="en">Bacillus coagulans</dc:subject>
	<dc:subject xml:lang="en">Rheumatoid</dc:subject>
	<dc:description xml:lang="en">Introduction: Rheumatoid arthritis (RA) is an autoimmune and systemic inflammatory disease influenced by microbial abnormalities (dysbiosis) in the intestinal that affect changes in metabolism and immune system disorders. This study aimed to investigate the predominant intestinal microbiota in complete Freund’s Adjuvant-induced rheumatoid arthritis rats after treated with caprine milk CSN1S2 protein through fecal analysis based on PCR-DGGE and to construct the phylogenetic tree of bacteria as the evolutionary relationship. Method: The experimental animals were divided into 6 groups with 2 types of rat model, namely control rat (C group, CM group, and CY group) and RA rat (RA group, RAM group, and RAY group). Predominant cultivable microbiota was obtained by direct culture and analyzed using PCR-DGGE with some specific primers. The DNA sequences were analyzed and aligned using bioinformatics software to construct the phylogenetic tree. Results: We found that Lactobacillus group significantly increased in the control rat model and the predominant intestinal bacteria in RA rats were Enterococcus group (Enterococcus faecium and Enterococcus faecalis). Conclusions: The caprine milk CSN1S2 protein influences the composition of microbiota in RA rats with the emergence of predominant bacteria that are considered species of the Bacillus group, closely related to Bacillus coagulans, which can be promoted the growth of B. coagulans to suppress pathogenic bacteria in the development of RA disease.</dc:description>
	<dc:publisher xml:lang="en">Journal of Tropical Life Science</dc:publisher>
	<dc:date>2021-06-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/1523</dc:identifier>
	<dc:identifier>10.11594/jtls.11.02.01</dc:identifier>
	<dc:source xml:lang="en">Journal of Tropical Life Science; Vol. 11 No. 2 (2021)</dc:source>
	<dc:source>2527-4376</dc:source>
	<dc:source>2087-5517</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/1523/pdf</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2021 Journal of Tropical Life Science</dc:rights>
</oai_dc:dc>