Melola Discovery

← Melola Discovery

Open access Journal SINTA 1

Monitoring the Success of Hydroseeding Application Using Microbial Diversity in Some Post-Coal Mining Revegetation Areas, South Kalimantan: Monitoring the Success of Hydroseeding Application Using Microbial Diversity

Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences · Vol. 14 No. 3 (2024) · pp. 489–500

DOI
10.11594/jtls.14.03.06
ISSN
2527-4376, 2087-5517
Published
2024-09-19
Publisher
Journal of Tropical Life Science
Language
eng
License
creativecommons.org/licenses/by-nc/4.0

Abstract

Successful revegetation improves the whole ecosystem's integrity, including microbe habitat. The goal of this study is to monitor the success of hydroseeding applications in some post-coal mining revegetation areas using microbial diversity. This study was conducted by sampling soils at a reference site (HS) and three revegetation areas (Kuni, AR07, AH). Kuni represents a 12-year-old monoculture replantation area while AR07 and AH are representations of hydroseeded areas with different ages, 5.5 years and 3 months old. Soil samples were then analyzed to determine N, P, K, and CEC, and extracted using the CTAB/SDS method, resulting in the total genome DNA. The genes of 16S rRNA were amplified using a specific primer, followed by product quantification and purification. Sequencing libraries were generated using the NEBNext® UltraTM DNA Library Pre Kit for Illumina. The library was sequenced on Illumina platform, and 250 bp paired-end reads were generated. The data were then analyzed using Anova, biplot, and cluster analysis. Results showed that AR07 was the most similar site to HS based on soil chemical properties and had the highest value of all alpha diversity indices. The dominant presence of Burkholderiales in AH indicated improvement in soil chemical properties. The soil microbial diversity PCA analysis revealed that the similarity between HS and AH was high. It was indicating a better success of hydroseeding at AH with shorter revegetation time as compared to the monoculture replantation site in Kuni. Hydroseeding using local pioneer species is highly recommended for postcoal mining revegetation.

Keywords

Collaboration network of the authors

40 authors · 82 co-authorship links · 1 across different organisations · showing the 40 most connected

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • University of Brawijaya
  • Universitas Halu Oleo
  • Institut Agama Islam Negeri Kendari
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Catur RetnaningdyahAffiliation unknown7
Kurniatun HairiahAffiliation unknown2
Chatarina Gradict SemiunAffiliation unknown2
Amin Setyo LeksonoAffiliation unknown2
Yuni Kartika DewiAffiliation unknown2
Wayan Firdaus MahmudyUniversity of Brawijaya1
Solimun SolimunUniversity of Brawijaya1
Candra DewiAffiliation unknown1
Budiman BudimanAffiliation unknown1
Depi NataliaAffiliation unknown1
S SoemarnoAffiliation unknown1
Daru Setyo RiniAffiliation unknown1
Donny HarisusenoAffiliation unknown1
Muhammad YusufUniversitas Halu Oleo1
Syahrul KurniawanUniversity of Brawijaya1
Adji Ahmad Rinaldo FernandesAffiliation unknown1
Adji Achmad Rinaldo FernandesUniversity of Brawijaya1
Muhammad Fadhil AnshariAffiliation unknown1
Yulia NurainiAffiliation unknown1
Siska NurfitrianiAffiliation unknown1
Jehan Ramdani HaryatiAffiliation unknown1
Intan N. AzizahAffiliation unknown1
Nurhidayati NurhidayatiAffiliation unknown1
Didik SuprayogoAffiliation unknown1
Setijono SaminoAffiliation unknown1
Ayu Raisa Khairun Nisa'Affiliation unknown1
Shinta ShintaInstitut Agama Islam Negeri Kendari1
Serafinah IndriyaniAffiliation unknown1
Eko WidodoAffiliation unknown1
Eufrasia Reneilda Arianti LengurAffiliation unknown1
Yoga Dwi JatmikoAffiliation unknown1
Irfan MustafaAffiliation unknown1
Durrotul InayahAffiliation unknown1
Nia KurniawanAffiliation unknown1
Syifa SaputraAffiliation unknown1
Titik WijayantiAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) http://jtrolis.ub.ac.id/index.php/jtrolis/oai, first harvested 9 Nov 2026, last seen 9 Nov 2026.
Original metadata (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Monitoring the Success of Hydroseeding Application Using Microbial Diversity in Some Post-Coal Mining Revegetation Areas, South Kalimantan: Monitoring the Success of Hydroseeding Application Using Microbial Diversity</dc:title>
	<dc:creator xml:lang="en"> Fadhil Anshari, Muhammad</dc:creator>
	<dc:creator xml:lang="en">Achmad Rinaldo Fernandes , Adji</dc:creator>
	<dc:creator xml:lang="en">Setyo Leksono, Amin</dc:creator>
	<dc:creator xml:lang="en">Arisoesilaningsih, Endang</dc:creator>
	<dc:subject xml:lang="en">Microbe diversity</dc:subject>
	<dc:subject xml:lang="en">NGS</dc:subject>
	<dc:subject xml:lang="en">Post-coal mining</dc:subject>
	<dc:subject xml:lang="en">Revegetation</dc:subject>
	<dc:subject xml:lang="en">Soil</dc:subject>
	<dc:description xml:lang="en">Successful revegetation improves the whole ecosystem's integrity, including microbe habitat. The goal of this study is to monitor the success of hydroseeding applications in some post-coal mining revegetation areas using microbial diversity. This study was conducted by sampling soils at a reference site (HS) and three revegetation areas (Kuni, AR07, AH). Kuni represents a 12-year-old monoculture replantation area while AR07 and AH are representations of hydroseeded areas with different ages, 5.5 years and 3 months old. Soil samples were then analyzed to determine N, P, K, and CEC, and extracted using the CTAB/SDS method, resulting in the total genome DNA. The genes of 16S rRNA were amplified using a specific primer, followed by product quantification and purification. Sequencing libraries were generated using the NEBNext® UltraTM DNA Library Pre Kit for Illumina. The library was sequenced on Illumina platform, and 250 bp paired-end reads were generated. The data were then analyzed using Anova, biplot, and cluster analysis. Results showed that AR07 was the most similar site to HS based on soil chemical properties and had the highest value of all alpha diversity indices. The dominant presence of Burkholderiales in AH indicated improvement in soil chemical properties. The soil microbial diversity PCA analysis revealed that the similarity between HS and AH was high. It was indicating a better success of hydroseeding at AH with shorter revegetation time as compared to the monoculture replantation site in Kuni. Hydroseeding using local pioneer species is highly recommended for postcoal mining revegetation.</dc:description>
	<dc:publisher xml:lang="en">Journal of Tropical Life Science</dc:publisher>
	<dc:date>2024-09-19</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/3216</dc:identifier>
	<dc:identifier>10.11594/jtls.14.03.06</dc:identifier>
	<dc:source xml:lang="en">Journal of Tropical Life Science; Vol. 14 No. 3 (2024); 489 – 500</dc:source>
	<dc:source>2527-4376</dc:source>
	<dc:source>2087-5517</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/3216/753</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2024 Journal of Tropical Life Science</dc:rights>
	<dc:rights xml:lang="en">https://creativecommons.org/licenses/by-nc/4.0</dc:rights>
</oai_dc:dc>