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Isolation, Screening, and Molecular Identification of Cellulolytic Bacteria From Supit Urang Municipal Landfill, Malang City: Identification of Cellulolytic Bacteria From Supit Urang Municipal Landfill

Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences · Vol. 14 No. 3 (2024) · pp. 585–592

DOI
10.11594/jtls.14.03.14
ISSN
2527-4376, 2087-5517
Published
2024-10-18
Publisher
Journal of Tropical Life Science
Language
eng
License
creativecommons.org/licenses/by-nc/4.0

Abstract

The municipal landfills contain substantial amounts of lignocellulosic waste that have not been adequately utilized. This waste can be processed using cellulolytic bacteria. Cellulolytic bacteria play an important role in the degradation of cellulose-based materials. This study aimed to isolate and identify cellulolytic bacteria based on 16S rDNA sequence obtained from the Supit Urang municipal landfill, Malang City. Bacteria were isolated from soil by cultivating on 1% carboxyl methyl cellulose (CMC) agar media. The cellulolytic activity was analyzed semi-quantitatively with flooded 1% Congo red and then washed with 1 M NaCl three times. Cellulase activity assay was measured using the 3.5-Dinitrosalicylic Acid (DNS) method. The most potential isolate in cellulose decomposition was identified. The most potential isolate in cellulose decomposition was identified. Twenty isolates of cellulolytic bacteria were obtained from the sample and sixteen isolates formed the clear zone. The cellulolytic index ranged between 0.26 and 1.52. Five isolates, SL1, SL2, SL10, SL16, and SL19 had the highest cellulolytic index. Isolate SL2 had the highest cellulase activity at 0.071 U/mL. Based on the 16S rDNA sequence, SL2 was identified as Bacillus amyloliquefaciens F98 with a similarity of 99.02%. This potential isolate has prospects for the biodegradation process from agricultural waste, which can be processed into valuable products.

Keywords

Collaboration network of the authors

40 authors · 103 co-authorship links · 1 across different organisations

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  • University of Brawijaya
  • Yogyakarta State University
  • Universitas Amikom Yogyakarta
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Yoga Dwi JatmikoAffiliation unknown2
Amin Setyo LeksonoAffiliation unknown1
Wildania Putri AgustinaAffiliation unknown1
Marsa SalsabilaAffiliation unknown1
Nabila Fitri RosyidaAffiliation unknown1
Riska Putri QurotaayuninaAffiliation unknown1
Nia KurniawanAffiliation unknown1
Anisa ZairinaAffiliation unknown1
Hakan BozdoğanAffiliation unknown1
Suharjono SuharjonoAffiliation unknown1
Evi OctavianyAffiliation unknown1
Elsa Mega SuryaniAffiliation unknown1
Hikmah MaulidiyahAffiliation unknown1
Salam A. IbrahimAffiliation unknown1
Edi IstiyonoYogyakarta State University1
Wipsar Brams DwandaruAffiliation unknown1
NurjanahAffiliation unknown1
Binawati GintingAffiliation unknown1
Ilham MaulanaAffiliation unknown1
Tasya UlfikaAffiliation unknown1
Dian SiswantoUniversity of Brawijaya1
Idham Halil ZakariaAffiliation unknown1
Siti Mariyah UlfaAffiliation unknown1
Anna SafitriAffiliation unknown1
Nishia Waya MerayAffiliation unknown1
Syukria I ZamAffiliation unknown1
Syamsuardi SyamsuardiAffiliation unknown1
Anthoni AgustienAffiliation unknown1
Syukria Ikhsan ZamAffiliation unknown1
Akmal DjamaanAffiliation unknown1
Afifah Nur AiniUniversitas Amikom Yogyakarta1
Rodiyati AzrianingsihUniversity of Brawijaya1
Endang ArisoesilaningsihAffiliation unknown1
Durrotul InayahAffiliation unknown1
Indah OktavianiAffiliation unknown1
Catur RetnaningdyahAffiliation unknown1
Viky VidayantiAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) http://jtrolis.ub.ac.id/index.php/jtrolis/oai, first harvested 9 Nov 2026, last seen 9 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en">Isolation, Screening, and Molecular Identification of Cellulolytic Bacteria From Supit Urang Municipal Landfill, Malang City: Identification of Cellulolytic Bacteria From Supit Urang Municipal Landfill</dc:title>
	<dc:creator xml:lang="en">Maghfira Selia</dc:creator>
	<dc:creator xml:lang="en">Irfan Mustafa</dc:creator>
	<dc:creator xml:lang="en">Farida Rahayu</dc:creator>
	<dc:subject xml:lang="en">Bacillus amyloliquefaciens F98</dc:subject>
	<dc:subject xml:lang="en">Cellulolytic bacteria</dc:subject>
	<dc:subject xml:lang="en">cellulase activity assay</dc:subject>
	<dc:description xml:lang="en">The municipal landfills contain substantial amounts of lignocellulosic waste that have not been adequately utilized. This waste can be processed using cellulolytic bacteria. Cellulolytic bacteria play an important role in the degradation of cellulose-based materials. This study aimed to isolate and identify cellulolytic bacteria based on 16S rDNA sequence obtained from the Supit Urang municipal landfill, Malang City. Bacteria were isolated from soil by cultivating on 1% carboxyl methyl cellulose (CMC) agar media. The cellulolytic activity was analyzed semi-quantitatively with flooded 1% Congo red and then washed with 1 M NaCl three times. Cellulase activity assay was measured using the 3.5-Dinitrosalicylic Acid (DNS) method. The most potential isolate in cellulose decomposition was identified. The most potential isolate in cellulose decomposition was identified. Twenty isolates of cellulolytic bacteria were obtained from the sample and sixteen isolates formed the clear zone. The cellulolytic index ranged between 0.26 and 1.52. Five isolates, SL1, SL2, SL10, SL16, and SL19 had the highest cellulolytic index. Isolate SL2 had the highest cellulase activity at 0.071 U/mL. Based on the 16S rDNA sequence, SL2 was identified as Bacillus amyloliquefaciens F98 with a similarity of 99.02%. This potential isolate has prospects for the biodegradation process from agricultural waste, which can be processed into valuable products.</dc:description>
	<dc:publisher xml:lang="en">Journal of Tropical Life Science</dc:publisher>
	<dc:date>2024-10-18</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
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	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/3314</dc:identifier>
	<dc:identifier>10.11594/jtls.14.03.14</dc:identifier>
	<dc:source xml:lang="en">Journal of Tropical Life Science; Vol. 14 No. 3 (2024); 585 – 592</dc:source>
	<dc:source>2527-4376</dc:source>
	<dc:source>2087-5517</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/3314/767</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2024 Journal of Tropical Life Science</dc:rights>
	<dc:rights xml:lang="en">https://creativecommons.org/licenses/by-nc/4.0</dc:rights>
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