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Assessing various administration strategies for dsRNA vaccine delivery: a concise review of VP15-WSSV research progress in tiger shrimp Penaeus monodon

Jurnal Ilmiah Perikanan dan Kelautan · Vol. 17 No. 3 (2025) · pp. 846–862

DOI
10.20473/jipk.v17i3.73580
ISSN
2528-0759, 2085-5842
Published
2025-07-17
Publisher
Faculty of Fisheries and Marine Universitas Airlangga
Language
eng
License
creativecommons.org/licenses/by-nc-sa/4.0

Abstract

Graphical Abstract Highlight Research 1. The dsRNA application should correspond to the developmental stages of shrimp2. Immersion method was commonly used for larvae for handling many individuals3. Injection technique effectively delivered dsRNA to cells but was unsuitable for large-scale4. Oral administration of pellet-enriched dsRNA was applicable to apply on shrimp larvae, juveniles, and broodstocks Abstract RNAi technology offers a novel powerful approach to silence gene expression by introducing double-stranded RNA (dsRNA) into the cell to degrade the mRNA at the post-transcriptional stage. An administration method of dsRNA delivery is one of the main considerations in applying the dsRNA vaccine for controlling pathogen infections. This mini-review was focused on the evaluation of three different methods (immersion, injection, and oral administration) of VP15-dsRNA vaccine delivery to the tiger shrimp post-WSSV challenge test. The immersion method was generally applied for the larval stage of tiger shrimp and seemed to be a simple technique for a large number of individuals in a small tank. The VP15-dsRNA application by immersion improved the survival of tiger shrimp larvae by 3.9% compared to the control groups. The injection technique was an effective way to deliver dsRNA to the cell, but it is difficult to apply in a large number of individuals or populations. The injection of VP15-dsRNA increased significantly the survival rate, proPO, and THC of tiger shrimp. A higher survival rate (75%) was exhibited in tiger shrimp injected with in vivo and in vitro VP15-dsRNA than in the control. Oral administration by pellet-enriched VP15-dsRNA was a useful way for larvae, juveniles, and broodstocks, but it has limitations since the pellet leaches into the water. The application of the VP15-dsRNA vaccine on the feed significantly enhanced the 26.7% higher survival rate compared to the control. The higher survival was also supported by a higher number of THC. The three VP15-dsRNA delivery methods provide potential approaches to increase tiger shrimp resistance to control pathogen infection

Keywords

Collaboration network of the authors

40 authors · 90 co-authorship links · 11 across different organisations · showing the 40 most connected

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • Universitas Halu Oleo
  • Universitas Handayani Makassar
  • Universitas Sulawesi Barat
  • Hasanuddin University
  • Universitas Airlangga
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Purbowatiningrum Ria SarjonoAffiliation unknown3
Abdul RokhmanUniversitas Halu Oleo2
Hazriani HazrianiAffiliation unknown2
Adnan NurUniversitas Handayani Makassar2
Baso HabibiUniversitas Handayani Makassar1
Abd RochmanUniversitas Handayani Makassar1
Ika NovitasariUniversitas Sulawesi Barat1
Andi Dewi PratiwiUniversitas Sulawesi Barat1
Nur Siti AisyahUniversitas Sulawesi Barat1
Yusnaini YusnainiUniversitas Halu Oleo1
La SaraUniversitas Halu Oleo1
Jopang JopangUniversitas Halu Oleo1
Tanzil TanzilUniversitas Halu Oleo1
Edy KarnoUniversitas Halu Oleo1
RAMLY RAMLYUniversitas Halu Oleo1
Ambo Wonua NusantaraAffiliation unknown1
Hasan AedyAffiliation unknown1
Wilma J.C. MokaAffiliation unknown1
Nita RukminasariHasanuddin University1
Andi Aliah HidayaniHasanuddin University1
Nur Indah Sari ArbitUniversitas Sulawesi Barat1
Sapto AndriyonoUniversitas Airlangga1
Noviatri IndahAffiliation unknown1
Frin EbenAffiliation unknown1
Sindi Mertisia BoutiAffiliation unknown1
M. Said ZainuddinAffiliation unknown1
Andi Mas Jaya AMAffiliation unknown1
Arifuddin UsmanAffiliation unknown1
Muhammad HarliawanAffiliation unknown1
Akbar SudirmanAffiliation unknown1
Intan Rosiana AriantiAffiliation unknown1
Pitriyani PitriyaniAffiliation unknown1
Firman FirmanHasanuddin University1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://e-journal.unair.ac.id/index.php/JIPK/oai, first harvested 9 Nov 2026, last seen 9 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en-US">Assessing various administration strategies for dsRNA vaccine delivery: a concise review of VP15-WSSV research progress in tiger shrimp Penaeus monodon</dc:title>
	<dc:creator>Parenrengi, Andi</dc:creator>
	<dc:creator>Lante, Samuel</dc:creator>
	<dc:creator>Suryati, Emma</dc:creator>
	<dc:creator>Rosmiati, Rosmiati</dc:creator>
	<dc:creator>Sulaeman, Sulaeman</dc:creator>
	<dc:creator>Herlinah, Herlinah</dc:creator>
	<dc:creator>Andriani, Yosie </dc:creator>
	<dc:subject xml:lang="en-US">delivery system</dc:subject>
	<dc:subject xml:lang="en-US">dsRNA vaccine</dc:subject>
	<dc:subject xml:lang="en-US">tiger shrimp</dc:subject>
	<dc:subject xml:lang="en-US">VP15-WSSV</dc:subject>
	<dc:description xml:lang="en-US">Graphical Abstract

 
 
Highlight Research
1. The dsRNA application should correspond to the developmental stages of shrimp2. Immersion method was commonly used for larvae for handling many individuals3. Injection technique effectively delivered dsRNA to cells but was unsuitable for large-scale4. Oral administration of pellet-enriched dsRNA was applicable to apply on shrimp larvae, juveniles, and broodstocks
 
 
Abstract
RNAi technology offers a novel powerful approach to silence gene expression by introducing double-stranded RNA (dsRNA) into the cell to degrade the mRNA at the post-transcriptional stage. An administration method of dsRNA delivery is one of the main considerations in applying the dsRNA vaccine for controlling pathogen infections. This mini-review was focused on the evaluation of three different methods (immersion, injection, and oral administration) of VP15-dsRNA vaccine delivery to the tiger shrimp post-WSSV challenge test. The immersion method was generally applied for the larval stage of tiger shrimp and seemed to be a simple technique for a large number of individuals in a small tank. The VP15-dsRNA application by immersion improved the survival of tiger shrimp larvae by 3.9% compared to the control groups. The injection technique was an effective way to deliver dsRNA to the cell, but it is difficult to apply in a large number of individuals or populations. The injection of VP15-dsRNA increased significantly the survival rate, proPO, and THC of tiger shrimp. A higher survival rate (75%) was exhibited in tiger shrimp injected with in vivo and in vitro VP15-dsRNA than in the control. Oral administration by pellet-enriched VP15-dsRNA was a useful way for larvae, juveniles, and broodstocks, but it has limitations since the pellet leaches into the water. The application of the VP15-dsRNA vaccine on the feed significantly enhanced the 26.7% higher survival rate compared to the control. The higher survival was also supported by a higher number of THC. The three VP15-dsRNA delivery methods provide potential approaches to increase tiger shrimp resistance to control pathogen infection</dc:description>
	<dc:publisher xml:lang="en-US">Faculty of Fisheries and Marine Universitas Airlangga</dc:publisher>
	<dc:date>2025-07-17</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:format>text/xml</dc:format>
	<dc:identifier>https://e-journal.unair.ac.id/JIPK/article/view/73580</dc:identifier>
	<dc:identifier>10.20473/jipk.v17i3.73580</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Ilmiah Perikanan dan Kelautan; Vol. 17 No. 3 (2025): JURNAL ILMIAH PERIKANAN DAN KELAUTAN; 846-862</dc:source>
	<dc:source>2528-0759</dc:source>
	<dc:source>2085-5842</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://e-journal.unair.ac.id/JIPK/article/view/73580/34138</dc:relation>
	<dc:relation>https://e-journal.unair.ac.id/JIPK/article/view/73580/35323</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2025 Jurnal Ilmiah Perikanan dan Kelautan</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc-sa/4.0</dc:rights>
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