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The Impact of Adding Egg Yolk In Various Concentrations into The Tyrode Extender on Muscovy Duck (Cairina Moschata) Sperm Quality

Jurnal Ilmu Ternak dan Veteriner · Vol. 30 No. 2 (2025) · pp. 107–113

DOI
10.14334/jitv.v30i2.3513
ISSN
2252-696X, 0853-7380
Published
2025-07-11
Publisher
APPERTANI
Language
eng

Abstract

):??-???. DOI:http://dx.doi.org/10.14334/jitv.v30i2.3513.This study investigated the impact of varying egg yolk concentrations in Tyrode extender on the quality of Muscovy duck semen after preservation. A sample of semen was collected from 1.5-year-old Muscovy ducks. The sample was evaluated for sperm motility, plasma membrane integrity, acrosome integrity, and sperm morphology before being separated into three equal parts and diluted in Tyrode extender containing increasing levels of egg yolk: T1 (5%), T2 (10%), and T3 (15%). After 21 hours of preservation at 4-5ºC, each sample was evaluated for the same parameters as fresh semen. The study found a significant decrease in sperm motility, membrane integrity, and acrosome integrity following preservation in T1, T2, and T3 compared to fresh ejaculate (53.34%; 63.5%; 60.04% vs 84.16%), (48.7%; 57.8%; 55.3% vs. 85.6%), and (50.6%; 57.6%; 56.1% vs 85.4%), respectively (P0.05). In contrast, abnormal sperm morphology increased considerably in T1, T2, and T3 compared to fresh semen (6.69%, 6.89%, 7.89% vs 2.2%) (P0.05). Furthermore, the addition of 10% egg yolk to the Tyrode extender resulted in the best percentages of sperm motility, sperm membrane integrity, and acrosome integrity following preservation. Surprisingly, the percentage of sperm with abnormal morphology did not differ significantly (P0.05) between T1, T2, and T3 after the preservation period. It can be concluded that adding 10% egg yolk to the extender substantially improved sperm motility, membrane integrity, and acrosome integrity in Muscovy duck sperm after 21 hours of preservation.

Keywords

Collaboration network of the authors

40 authors · 100 co-authorship links · 29 across different organisations · showing the 40 most connected

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • Padjadjaran University
  • Satya Wacana Christian University
  • Universitas Buana Perjuangan Karawang
  • Institut Agama Islam Negeri Kerinci
  • STMIK IKMI Cirebon
  • University of Nusa Cendana
  • Universitas Halu Oleo
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Dudang GojaliAffiliation unknown5
Doli WitroInstitut Agama Islam Negeri Kerinci2
Ade PonirahAffiliation unknown2
Rahayu KusumadewiAffiliation unknown2
Rani MulyaniAffiliation unknown2
Abdulah SafeiAffiliation unknown2
Lies SulistyowatiAffiliation unknown2
Dedy HamdaniAffiliation unknown2
Maria Teodora PingAffiliation unknown2
Effendi LimbongAffiliation unknown2
Aryasatya Abdie MaheswaraAffiliation unknown1
Hindriyanto Dwi PurnomoSatya Wacana Christian University1
Irwan SembiringSatya Wacana Christian University1
Danny ManonggaSatya Wacana Christian University1
Ahmad FauziSTMIK IKMI Cirebon1
Baenil HudaSatya Wacana Christian University1
Hendry HendrySatya Wacana Christian University1
April Lia HanantoUniversitas Buana Perjuangan Karawang1
Tukino TukinoUniversitas Buana Perjuangan Karawang1
Petrus KuneUniversity of Nusa Cendana1
Takdir SailiUniversitas Halu Oleo1
Dini widianingrumAffiliation unknown1
Tuti WidjastutiAffiliation unknown1
Asep AnangAffiliation unknown1
Abun AbunAffiliation unknown1
Abdul Zainal MuttaqinAffiliation unknown1
Nurcholidah SolihatiPadjadjaran University1
Soeparna SoeparnaPadjadjaran University1
Rangga SetiawanPadjadjaran University1
Annisaa YusrinaPadjadjaran University1
Tazkia KhaerunnisaPadjadjaran University1
Lucyana TrimoPadjadjaran University1
Gema Wibawa MuktiPadjadjaran University1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://medpub.appertani.org/index.php/jitv/oai, first harvested 9 Nov 2026, last seen 9 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en-US">The Impact of Adding Egg Yolk In Various Concentrations into The Tyrode Extender on Muscovy Duck (Cairina Moschata) Sperm Quality</dc:title>
	<dc:creator>Rasad, Siti Darodjah</dc:creator>
	<dc:creator>Widyastuti, Rini</dc:creator>
	<dc:creator>Setiawan, Iwan</dc:creator>
	<dc:creator>Sujana, Endang</dc:creator>
	<dc:creator>Setiawan, Rangga</dc:creator>
	<dc:creator>Solihati, Nurcholidah</dc:creator>
	<dc:creator>Mohd. Nadzir, Mohd. Noor Hisham</dc:creator>
	<dc:subject xml:lang="en-US">Egg Yolk; Preservation; Sperm Quality; Thyrode</dc:subject>
	<dc:description xml:lang="en-US">):??-???. DOI:http://dx.doi.org/10.14334/jitv.v30i2.3513.This study investigated the impact of varying egg yolk concentrations in Tyrode extender on the quality of Muscovy duck semen after preservation.  A sample of semen was collected from 1.5-year-old Muscovy ducks.  The sample was evaluated for sperm motility, plasma membrane integrity, acrosome integrity, and sperm morphology before being separated into three equal parts and diluted in Tyrode extender containing increasing levels of egg yolk: T1 (5%), T2 (10%), and T3 (15%).  After 21 hours of preservation at 4-5ºC, each sample was evaluated for the same parameters as fresh semen.  The study found a significant decrease in sperm motility, membrane integrity, and acrosome integrity following preservation in T1, T2, and T3 compared to fresh ejaculate (53.34%; 63.5%; 60.04% vs 84.16%), (48.7%; 57.8%; 55.3% vs. 85.6%), and (50.6%; 57.6%; 56.1% vs 85.4%), respectively (P0.05).  In contrast, abnormal sperm morphology increased considerably in T1, T2, and T3 compared to fresh semen (6.69%, 6.89%, 7.89% vs 2.2%) (P0.05).  Furthermore, the addition of 10% egg yolk to the Tyrode extender resulted in the best percentages of sperm motility, sperm membrane integrity, and acrosome integrity following preservation.  Surprisingly, the percentage of sperm with abnormal morphology did not differ significantly (P0.05) between T1, T2, and T3 after the preservation period.  It can be concluded that adding 10% egg yolk to the extender substantially improved sperm motility, membrane integrity, and acrosome integrity in Muscovy duck sperm after 21 hours of preservation.</dc:description>
	<dc:publisher xml:lang="en-US">APPERTANI</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2025-07-11</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
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	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://medpub.appertani.org/index.php/jitv/article/view/3513</dc:identifier>
	<dc:identifier>10.14334/jitv.v30i2.3513</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Ilmu Ternak dan Veteriner; Vol 30, No 2 (2025): June 2025; 107-113</dc:source>
	<dc:source>2252-696X</dc:source>
	<dc:source>0853-7380</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://medpub.appertani.org/index.php/jitv/article/view/3513/pdf</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2025 Jurnal Ilmu Ternak dan Veteriner</dc:rights>
</oai_dc:dc>