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Single nucleotide polymorphism of prolactin gene exon two in ducks of Pekin, Mojosari and Pekin Mojosari crossbred

Jurnal Ilmu Ternak dan Veteriner · Vol. 19 No. 2 (2014)

DOI
10.14334/jitv.v19i2.1038
ISSN
2252-696X, 0853-7380
Published
2014-09-19
Publisher
APPERTANI
Language
eng
License
creativecommons.org/licenses/by/4.0

Abstract

Prolactin gene plays crucial role in the reproduction and egg production of birds. The objectives of this study were to characterize single nucleotide polymorphism in partial intron and coding region of duck prolactin gene. Blood samples were collected from 168 ducks consisted of 19 Pekin, 36 Mojosari, and 113 of their crossbreds collected from Indonesian Research Institute for Animal Production (IRIAP). Primer pairs for the coding regions in prolactin gene were self designed based on the duck genomic sequence database (GeneBank: AB158611.1). PCR products based on DNA of prolactin gene exon two was amplified approximately 400 bp. There is one base insertion of Adenin at the position of 2001 bp intron two region of duck prolactin. Homology test based on BLAST method indicated 99% identity with duck refference (Code Access GeneBank: AB158611.1). Adenin composition in all of duck samples was higher than refference. Triplet hydrogen bonds between Guanine and Cytosin pairs was higher than those at duplet hydrogen bonds between Adenine and Thymine. All duck samples were homozigous and monomorphyc.

Keywords

Collaboration network of the authors

40 authors · 94 co-authorship links · 11 across different organisations · showing the 40 most connected

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • IPB University
  • Universitas Halu Oleo
  • Universitas Tribhuwana Tunggadewi
  • Hasanuddin University
  • Diponegoro University
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Asep GunawanIPB University7
Anneke AnggraeniAffiliation unknown5
Ratna Sholatia HarahapIPB University4
Jakaria JakariaIPB University4
Kasita ListyariniIPB University3
Sri MurtiniIPB University3
Isyana KhaerunnisaAffiliation unknown3
L. Hardi PrasetyoAffiliation unknown3
Ronny Rachman NoorAffiliation unknown3
Niken UlupiIPB University2
Nena HilmiaAffiliation unknown2
Irma Isnafia AriefAffiliation unknown2
I KhaerunnisaAffiliation unknown2
E AndreasAffiliation unknown2
SubandriyoAffiliation unknown2
Eko HandiwirawanAffiliation unknown2
Harleli HarleliUniversitas Halu Oleo1
Swaidatul Masluhiya AfUniversitas Tribhuwana Tunggadewi1
Salman YusufAffiliation unknown1
Sri RahayuHasanuddin University1
Sutikno SutiknoDiponegoro University1
Rudy PriyantoIPB University1
Adinda Wanda AstiraAffiliation unknown1
Mutasem Ali M. AbuzahraAffiliation unknown1
Melly Pratiwi SetyawatiIPB University1
Dewi Apri AstutiIPB University1
Elmy MarianaIPB University1
Achmad Gitaraka ArchimilarIPB University1
Sri DarwatiAffiliation unknown1
Irma IrmaUniversitas Halu Oleo1
Siti Darodjah RasadAffiliation unknown1
Sari SuryanahAffiliation unknown1
Vista BudiariatiAffiliation unknown1
Noer Muhammad Dliyaul HaqAffiliation unknown1
Diah PristihadiAffiliation unknown1
B PurwantaraAffiliation unknown1
D D SolihinAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://medpub.appertani.org/index.php/jitv/oai, first harvested 9 Nov 2026, last seen 9 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en-US">Single nucleotide polymorphism of prolactin gene exon two in ducks of Pekin, Mojosari and Pekin Mojosari crossbred</dc:title>
	<dc:creator>., Irma</dc:creator>
	<dc:creator>Sumantri, Cece</dc:creator>
	<dc:creator>Susanti, Triana</dc:creator>
	<dc:subject xml:lang="en-US">Prolactin; Mojosari; Pekin; Crossbred</dc:subject>
	<dc:subject xml:lang="en-US"></dc:subject>
	<dc:description xml:lang="en-US">Prolactin gene plays crucial role in the reproduction and egg production of birds. The objectives of this study were to characterize single nucleotide polymorphism in partial intron and coding region of duck prolactin gene. Blood samples were collected from 168 ducks consisted of 19 Pekin, 36 Mojosari, and 113 of their crossbreds collected from Indonesian Research Institute for Animal Production (IRIAP). Primer pairs for the coding regions in prolactin gene were self designed based on the duck genomic sequence database (GeneBank: AB158611.1). PCR products based on DNA of prolactin gene exon two was amplified approximately 400 bp. There is one base insertion of Adenin at the position of 2001 bp intron two region of duck prolactin. Homology test based on BLAST method indicated 99% identity with duck refference (Code Access GeneBank: AB158611.1). Adenin composition in all of duck samples was higher than refference. Triplet hydrogen bonds between Guanine and Cytosin pairs was higher than those at duplet hydrogen bonds between Adenine and Thymine. All duck samples were homozigous and monomorphyc.</dc:description>
	<dc:publisher xml:lang="en-US">APPERTANI</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2014-09-19</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
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	<dc:format>text/html</dc:format>
	<dc:identifier>https://medpub.appertani.org/index.php/jitv/article/view/1038</dc:identifier>
	<dc:identifier>10.14334/jitv.v19i2.1038</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Ilmu Ternak dan Veteriner; Vol 19, No 2 (2014): JUNE 2014; p.104-111</dc:source>
	<dc:source>2252-696X</dc:source>
	<dc:source>0853-7380</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://medpub.appertani.org/index.php/jitv/article/view/1038/pdf</dc:relation>
	<dc:relation>https://medpub.appertani.org/index.php/jitv/article/view/1038/1067</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 1970 Indonesian Journal of Animal and Veterinary Sciences</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by/4.0</dc:rights>
</oai_dc:dc>