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Effect of glutathione and bovine seminal plasma in lactose extender on viability of swamp buffalo frozen semen

Jurnal Ilmu Ternak dan Veteriner · Vol. 17 No. 3 (2013)

DOI
10.14334/jitv.v17i3.697
ISSN
2252-696X, 0853-7380
Published
2013-01-03
Publisher
APPERTANI
Language
eng
License
creativecommons.org/licenses/by/4.0

Abstract

The aim of the study was to investigate the effect on viability of frozen swamp buffalo semen of glutathione and bovine seminal plasma in lactose extender. Semen from two swamp buffalo bulls was collected twice weekly using an artificial vagina. Pooled, good-quality fresh semen was divided into three parts and centrifuged at 3,000 rpm for 15 minutes in preparation for three treatments-substitution of buffalo seminal plasma with zero, 50 or 100% bovine seminal plasma (BS0, BS50 and BS100, respectively). Each semen aliquot was then divided in two parts, on which was diluted with lactose extender containing 1 mM glutathione (GSH) and the other diluted with lactose extender without GSH (0 mM GSH). Extended semen from all six treatments was cooled to 5oC and then frozen in 0.25 ml straws. Mean motility percentages 0 and 30 minutes post thaw (PTM 0′ and 30′) with GSH were 38.33 and 34.29%, significantly higher (P 0.05) than treatments without GSH (31.67 and 25.95%). PTM 0′ and 30′ were also higher (P 0.05) with no substitution of bovine seminal plasma (BS0) than when buffalo seminal plasma was replaced with bovine seminal plasma at either 50 or 100%. Averages were 40.00 vs 34.46 and 30.54% (BS0 vs BS50 and BS100) at thawing and 36.96 vs 28.36 and 25.36% 30 minutes post-thaw. Mean percentages of live sperm (LD), intact plasma membrane (MPU) and intact acrosomal membrane (TAU) at thawing were not significantly different with or without addition of GSH. However,at 30′ post thawing, TAU and MPU were significantly higher in GSH treatments than inthose without GSH: 61.50 vs. 58.19% (MPU) and 59.81 vs. 57.38% (TAU). Mean percentages of LD, TAU and MPU 30′post thawing were higher with no substitution ofbuffalo seminal plasma (BS0) (P 0.05) than to BS50 and BS100 treatments. In conclusion, the addition of glutathione (GSH) improved the quality of frozen swamp buffalo semen, but the partial substitution of buffalo seminal plasma with bovine seminal plasmaprovided no beneficial effects. Key Words: Swamp buffalo, Semen, Antioxidant, Glutathione, Seminal plasma

Collaboration network of the authors

40 authors · 130 co-authorship links · showing the 40 most connected

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  • Universitas Halu Oleo
  • IPB University
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
T.L YusufAffiliation unknown8
M.R. ToelihereAffiliation unknown6
T SugiartiAffiliation unknown5
M AgilAffiliation unknown5
R.G SianturiAffiliation unknown4
D.A KusumaningrumAffiliation unknown4
M.A SetiadiAffiliation unknown4
Muhammad RizalUniversitas Halu Oleo4
A BoedionoAffiliation unknown4
E TriwulanningsihAffiliation unknown3
C. SumantriAffiliation unknown3
R.R. NoorAffiliation unknown3
E.M KaiinAffiliation unknown3
R.I ArifiantiniAffiliation unknown3
I.G PutuAffiliation unknown3
Bagus Priyo PurwantoAffiliation unknown2
Wasmen ManaluAffiliation unknown2
Fitra Aji PamungkasAffiliation unknown2
A.R. SetiokoAffiliation unknown2
I-K SutamaAffiliation unknown2
D. SajuthiAffiliation unknown2
I ArifiantiniAffiliation unknown2
YudiAffiliation unknown2
E.D GunardiAffiliation unknown2
K DiwyantoAffiliation unknown2
J.J RutledgeAffiliation unknown2
N. W. K. KarjaAffiliation unknown2
F. A. SatrioAffiliation unknown2
M. GunawanAffiliation unknown2
Endang TriwulaninngsihAffiliation unknown2
Ahmad YaniAffiliation unknown1
Asep SudarmanAffiliation unknown1
Siti Nurma IndahAffiliation unknown1
Cece SumantriIPB University1
D D SolihinAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://medpub.appertani.org/index.php/jitv/oai, first harvested 9 Nov 2026, last seen 9 Nov 2026.
Original metadata (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Effect of glutathione and bovine seminal plasma in lactose extender on viability of swamp buffalo frozen semen</dc:title>
	<dc:creator>Sianturi, Riasari Gail</dc:creator>
	<dc:creator>Purwantara, B</dc:creator>
	<dc:creator>Supriatna, I</dc:creator>
	<dc:creator>., Amrozi</dc:creator>
	<dc:creator>Situmorang, P</dc:creator>
	<dc:description xml:lang="en-US">The aim of the study was to investigate the effect on viability of frozen swamp buffalo semen of glutathione and bovine seminal plasma in lactose extender. Semen from two swamp buffalo bulls was collected twice weekly using an artificial vagina. Pooled, good-quality fresh semen was divided into three parts and centrifuged at 3,000 rpm for 15 minutes in preparation for three treatments-substitution of buffalo seminal plasma with zero, 50 or 100% bovine seminal plasma (BS0, BS50 and BS100, respectively). Each semen aliquot was then divided in two parts, on which was diluted with lactose extender containing 1 mM glutathione (GSH) and the other diluted with lactose extender without GSH (0 mM GSH). Extended semen from all six treatments was cooled to 5oC and then frozen in 0.25 ml straws.  Mean motility percentages 0 and 30 minutes post thaw (PTM 0′ and 30′) with GSH were 38.33 and 34.29%, significantly higher (P  0.05) than treatments without GSH (31.67 and 25.95%). PTM 0′ and 30′ were also higher (P  0.05) with no substitution of bovine seminal plasma (BS0) than when buffalo seminal plasma was replaced with bovine seminal plasma at either 50 or 100%. Averages were 40.00 vs 34.46 and 30.54% (BS0 vs BS50 and BS100) at thawing and 36.96 vs 28.36 and 25.36% 30 minutes post-thaw. Mean percentages of live sperm (LD), intact plasma membrane (MPU) and intact acrosomal membrane (TAU) at thawing were not significantly different with or without addition of GSH. However,at 30′ post thawing, TAU and MPU were significantly higher in GSH treatments than inthose without GSH:  61.50 vs. 58.19% (MPU) and 59.81 vs. 57.38% (TAU). Mean percentages of LD, TAU and MPU 30′post thawing were higher with no substitution ofbuffalo seminal plasma (BS0) (P  0.05) than to BS50 and BS100 treatments. In conclusion, the addition of glutathione (GSH) improved the quality of frozen swamp buffalo semen, but the partial substitution of buffalo seminal plasma with bovine seminal plasmaprovided no beneficial effects. Key Words: Swamp buffalo, Semen, Antioxidant, Glutathione, Seminal plasma</dc:description>
	<dc:publisher xml:lang="en-US">APPERTANI</dc:publisher>
	<dc:date>2013-01-03</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://medpub.appertani.org/index.php/jitv/article/view/697</dc:identifier>
	<dc:identifier>10.14334/jitv.v17i3.697</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Ilmu Ternak dan Veteriner; Vol 17, No 3 (2012): SEPTEMBER 2012; p.169-178</dc:source>
	<dc:source>2252-696X</dc:source>
	<dc:source>0853-7380</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://medpub.appertani.org/index.php/jitv/article/view/697/706</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 1970 Indonesian Journal of Animal and Veterinary Sciences</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by/4.0</dc:rights>
</oai_dc:dc>