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Evaluation of Motility, Viability, and Integrity Plasma Membranes of Frozen Semen in Friesian Holstein with Storage Periods of 33, 30, 27, and 24 Years

Jurnal Medik Veteriner · Vol. 6 No. 2 (2023) · pp. 162–172

DOI
10.20473/jmv.vol6.iss2.2023.162-172
ISSN
2581-012X, 2615-7497
Published
2023-10-22
Publisher
Universitas Airlangga
Language
eng
License
creativecommons.org/licenses/by-nc-sa/4.0

Abstract

The quality of frozen semen is one of the components of the artificial insemination program. The freezing method, kind of diluent, handling of frozen semen, and thawing process all affect the quality of frozen semen. This study aimed to determine the motility, viability, and plasma membrane integrity of frozen semen of Friesian Holstein cattle at storage periods of 33, 30, 27, and 24 years. The samples used were stored for 33 years (production 1987), 30 years (production 1990), 27 (1993 production), and 24 years (1996 production) stored in Singosari Artificial Insemination Center, Malang. Data were statistically analyzed using One-Way ANOVA followed by Duncan's Multiple Range Test (p < 0,05). Based on the study's findings, it can be concluded that the motility, viability, and plasma membrane integrity of frozen semen that had been preserved for 33, 30, 27, and 24 years were still acceptable and in good condition in comparison to the SNI quality criteria for bull frozen semen in Indonesia.

Keywords

Collaboration network of the authors

40 authors · 209 co-authorship links · 5 across different organisations · showing the 40 most connected

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • Universitas Islam Negeri Antasari Banjarmasin
  • Diponegoro University
  • Universitas Halu Oleo
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Tri Wahyu SuprayogiAffiliation unknown9
Ragil Angga PrastiyaAffiliation unknown8
Herry Agoes HermadiAffiliation unknown8
Sri Pantja MadyawatiAffiliation unknown6
Pudji SriantoAffiliation unknown6
Tita Damayanti LestariAffiliation unknown5
Aditya YudhanaAffiliation unknown5
Wildani HefniAffiliation unknown4
Bodhi AgustonoAffiliation unknown4
Amung Logam SaputroAffiliation unknown4
Maya Nurwartanti YunitaAffiliation unknown4
Budi UtomoDiponegoro University4
Widjiati WidjiatiAffiliation unknown4
Umar ChamdanAffiliation unknown3
Mutasem AbuzahraAffiliation unknown3
Trilas SardjitoAffiliation unknown3
Mirni LamidAffiliation unknown3
Iwan Sahrial HamidAffiliation unknown3
Tjuk Imam RestiadiAffiliation unknown3
Ibnu Akbar MalikiAffiliation unknown2
Rizqa AhmadiAffiliation unknown2
Sri HidanahAffiliation unknown2
Seagames WaluyoAffiliation unknown2
Heni PuspitasariAffiliation unknown2
Epy Muhammad LuqmanAffiliation unknown2
Dicky Mohammad DikmanAffiliation unknown2
Wiyanto HaditanojoAffiliation unknown2
Prima Ayu WibawatiAffiliation unknown2
Widya Paramita LokapirnasariAffiliation unknown2
Ainul Hayatin NisaUniversitas Islam Negeri Antasari Banjarmasin1
Muhaimin MuhaiminUniversitas Islam Negeri Antasari Banjarmasin1
Fithriana SyarqawieUniversitas Islam Negeri Antasari Banjarmasin1
Ilham AkbarUniversitas Halu Oleo1
Evi SopiahAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://e-journal.unair.ac.id/JMV/oai, first harvested 9 Nov 2026, last seen 9 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en-US">Evaluation of Motility, Viability, and Integrity Plasma Membranes of Frozen Semen in Friesian Holstein with Storage Periods of 33, 30, 27, and 24 Years</dc:title>
	<dc:creator>Purnawan, Ahmad Budi</dc:creator>
	<dc:creator>Rimayanti, Rimayanti</dc:creator>
	<dc:creator>Susilowati, Suherni</dc:creator>
	<dc:creator>Mustofa, Imam</dc:creator>
	<dc:creator>Hernawati, Tatik</dc:creator>
	<dc:creator>Safitri, Erma</dc:creator>
	<dc:subject xml:lang="en-US">Friesian Holstein</dc:subject>
	<dc:subject xml:lang="en-US">motility</dc:subject>
	<dc:subject xml:lang="en-US">plasma membrane integrity</dc:subject>
	<dc:subject xml:lang="en-US">storage periods</dc:subject>
	<dc:subject xml:lang="en-US">viability</dc:subject>
	<dc:description xml:lang="en-US">The quality of frozen semen is one of the components of the artificial insemination program. The freezing method, kind of diluent, handling of frozen semen, and thawing process all affect the quality of frozen semen. This study aimed to determine the motility, viability, and plasma membrane integrity of frozen semen of Friesian Holstein cattle at storage periods of 33, 30, 27, and 24 years. The samples used were stored for 33 years (production 1987), 30 years (production 1990), 27 (1993 production), and 24 years (1996 production) stored in Singosari Artificial Insemination Center, Malang. Data were statistically analyzed using One-Way ANOVA followed by Duncan's Multiple Range Test (p &amp;lt; 0,05). Based on the study's findings, it can be concluded that the motility, viability, and plasma membrane integrity of frozen semen that had been preserved for 33, 30, 27, and 24 years were still acceptable and in good condition in comparison to the SNI quality criteria for bull frozen semen in Indonesia.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Airlangga</dc:publisher>
	<dc:date>2023-10-22</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://e-journal.unair.ac.id/JMV/article/view/34305</dc:identifier>
	<dc:identifier>10.20473/jmv.vol6.iss2.2023.162-172</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Medik Veteriner; Vol. 6 No. 2 (2023): October; 162-172</dc:source>
	<dc:source>2581-012X</dc:source>
	<dc:source>2615-7497</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://e-journal.unair.ac.id/JMV/article/view/34305/26429</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2023 Ahmad Budi Purnawan, Rimayanti Rimayanti, Suherni Susilowati, Imam Mustofa, Tatik Hernawati, Erma Safitri</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc-sa/4.0</dc:rights>
</oai_dc:dc>