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Phenotypic and Genotypic Detection of Staphylococcus aureus and Escherichia coli in Subclinical Mastitis Goat Milk in Lumajang, Indonesia

Jurnal Medik Veteriner · Vol. 8 No. 1 (2025) · pp. 167–174

DOI
10.20473/jmv.vol8.iss1.2025.167-174
ISSN
2581-012X, 2615-7497
Published
2025-04-01
Publisher
Universitas Airlangga
Language
eng
License
creativecommons.org/licenses/by-nc-sa/4.0

Abstract

This study aimed to identify the Staphylococcus aureus and Escherichia coli bacteria that cause subclinical mastitis in dairy goats in the Senduro sub-district of Lumajang regency, Indonesia. A total of 116 milk samples from 58 dairy goats were tested for the California Mastitis Test (CMT). CMT-positive samples were used for bacterial identification through phenotypic and genotypic analyses. Phenotypic analysis was conducted by culturing bacteria on natrium agar media and followed in selective media. The genotypic analysis employed a specific primer of S. aureus using the 23SrRNA gene and E. coli using the 16SrRNA gene as confirmation of the gold standart. The results of this study showed positive subclinical mastitis in dairy goat samples, which consisted of 21 samples of CMT 1 and a sample of CMT 2. Bacterial identification with phenotypic assays documented 16 samples having S. aureus and one sample having E. coli. The validated genotypic assays showed from 16 samples, validated 5 samples of S. aureus infection and one E. coli infection. In conclusion, bacterial identification of S. aureus and E. coli predominantly infects the SCM dairy goats in the Senduro sub-district as evidenced by the phenotypic analysis and followed by genotypic using the PCR method.

Keywords

Collaboration network of the authors

40 authors · 132 co-authorship links · showing the 40 most connected

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  • University of Mataram
  • Universitas Jember
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Salnida Yuniarti LumbessyUniversity of Mataram1
Fariq AzharUniversity of Mataram1
Dina ErlianaAffiliation unknown1
Munifilia EkasariAffiliation unknown1
Sindi HaryaniAffiliation unknown1
Uni BarorohAffiliation unknown1
MeylandaAffiliation unknown1
Tio Ersa RelyansyahAffiliation unknown1
Muhammad Irfan Nur WidadAffiliation unknown1
Fernando Mersa PutraAffiliation unknown1
Corry SorminAffiliation unknown1
Khairul AlimAffiliation unknown1
Cut MultahadahAffiliation unknown1
Safni SafniAffiliation unknown1
Diana Vanda WelliaAffiliation unknown1
Dina NofebrianiAffiliation unknown1
Tri RatnasariAffiliation unknown1
Arif Mohammad SiddiqAffiliation unknown1
Agung Sih KurniantoAffiliation unknown1
Nilasari DewiAffiliation unknown1
Hari SulistyowatiAffiliation unknown1
Husnul KhowatiniAffiliation unknown1
Anggun Sausan FirdausAffiliation unknown1
Fariz Kustiawan AlfarisyAffiliation unknown1
Sigit SoeparjonoAffiliation unknown1
Tri Wahyu SaputraAffiliation unknown1
Laily Ilman WiduriAffiliation unknown1
Evita Soliha HaniUniversitas Jember1
Wildan MuhlisonAffiliation unknown1
Tuti HaryatiAffiliation unknown1
TRESNAWATI PURWADARIAAffiliation unknown1
Arnold Parlindungan SinuratAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://e-journal.unair.ac.id/JMV/oai, first harvested 9 Nov 2026, last seen 9 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en-US">Phenotypic and Genotypic Detection of Staphylococcus aureus and Escherichia coli in Subclinical Mastitis Goat Milk in Lumajang, Indonesia</dc:title>
	<dc:creator>Khasanah, Himmatul</dc:creator>
	<dc:creator>Putra, Nalendra Gigih Wibawanto</dc:creator>
	<dc:creator>Hariyadi, Nanang Tri</dc:creator>
	<dc:creator>Widodo, Nur</dc:creator>
	<dc:creator>Widianingrum, Desy</dc:creator>
	<dc:creator>Yulianto, Roni</dc:creator>
	<dc:creator>Cruz, Joseph F. Dela</dc:creator>
	<dc:creator>Pratiwi, Nurul</dc:creator>
	<dc:subject xml:lang="en-US">bacterial infections</dc:subject>
	<dc:subject xml:lang="en-US">gram-negative bacteria</dc:subject>
	<dc:subject xml:lang="en-US">gram-positive bacteria</dc:subject>
	<dc:subject xml:lang="en-US">mastitis</dc:subject>
	<dc:subject xml:lang="en-US">SCM goat</dc:subject>
	<dc:description xml:lang="en-US">This study aimed to identify the Staphylococcus aureus and Escherichia coli bacteria that cause subclinical mastitis in dairy goats in the Senduro sub-district of Lumajang regency, Indonesia. A total of 116 milk samples from 58 dairy goats were tested for the California Mastitis Test (CMT). CMT-positive samples were used for bacterial identification through phenotypic and genotypic analyses. Phenotypic analysis was conducted by culturing bacteria on natrium agar media and followed in selective media. The genotypic analysis employed a specific primer of S. aureus using the 23SrRNA gene and E. coli using the 16SrRNA gene as confirmation of the gold standart. The results of this study showed positive subclinical mastitis in dairy goat samples, which consisted of 21 samples of CMT 1 and a sample of CMT 2. Bacterial identification with phenotypic assays documented 16 samples having S. aureus and one sample having E. coli. The validated genotypic assays showed from 16 samples, validated 5 samples of S. aureus infection and one E. coli infection. In conclusion, bacterial identification of S. aureus and E. coli predominantly infects the SCM dairy goats in the Senduro sub-district as evidenced by the phenotypic analysis and followed by genotypic using the PCR method.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Airlangga</dc:publisher>
	<dc:date>2025-04-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://e-journal.unair.ac.id/JMV/article/view/57799</dc:identifier>
	<dc:identifier>10.20473/jmv.vol8.iss1.2025.167-174</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Medik Veteriner; Vol. 8 No. 1 (2025): April; 167-174</dc:source>
	<dc:source>2581-012X</dc:source>
	<dc:source>2615-7497</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://e-journal.unair.ac.id/JMV/article/view/57799/32278</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2025 Himmatul Khasanah, Nalendra Gigih Wibawanto Putra, Nanang Tri Hariyadi, Nur Widodo, Desy Widianingrum, Roni Yulianto, Joseph F. Dela Cruz, Nurul Pratiwi</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc-sa/4.0</dc:rights>
</oai_dc:dc>